| EAST TENNESSEE HUMAN RESOURCE | Knoxville | — | $50M | 3 |
| Roane County Medical Center | Knoxville | E32 Health Care | $49.9M | 12 |
| NORTH CENTRAL TELEPHONE | Lafayette | — | $49.7M | 11 |
| RETIREE RESOURCES CORPORATION | Knoxville | M202 Public Safety, Disaster Preparedness & Relief | $49.3M | 7 |
| CENTERSTONE OF AMERICA INC | Nashville | F99 Mental Health & Crisis Intervention | $49.1M | 17 |
| LEAD PUBLIC SCHOOLS INC | Nashville | B29 Education | $48.9M | 9 |
| ISHA FOUNDATION INC | McMinnville | B60 Education | $48.7M | 0 |
| CCA of Tennessee LLC Employee Benefit Trust | Nashville | Y43 Mutual & Membership Benefit | $48.6M | 1 |
| Fort Loudoun Medical Center | Knoxville | E22Z Health Care | $47.9M | 11 |
| UNIVERSITY OF MEMPHIS FOUNDATION | Memphis | B110 Education | $47.6M | 2 |
| BAPTIST MEMORIAL HEALTH CARE | Memphis | E110 Health Care | $47.5M | 5 |
| HOOSIER CARE INC | Nashville | — | $47.3M | 8 |
| KNOX AREA RESCUE MINISTRIES INC | Knoxville | L410 Housing & Shelter | $46.9M | 2 |
| NATIONAL ASSOCIATION OF STATE BOARDS OF ACCOUNTANCY INC | Nashville | — | $46.7M | 11 |
| SECOND HARVEST FOOD BANK OF EAST | Maryville | — | $46.6M | 1 |
| Smyth County Community Hospital | Johnson City | E220 Health Care | $46.3M | 15 |
| UNITED WAY OF MIDDLE TENNESSEE INC | Nashville | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $46.2M | 11 |
| BETHEL UNIVERSITY | McKenzie | B43Z Education | $45.9M | 10 |
| TELLICO VILLAGE PROPERTY OWNERS | Loudon | L50 Housing & Shelter | $45M | 6 |
| THE ORANGE GROVE CENTER INC | Chattanooga | P82Z Human Services | $44.8M | 7 |
| BLOOD ASSURANCE INC | Chattanooga | E61Z Health Care | $44.6M | 8 |
| MEMPHIS BROOKS MUSEUM OF ART INC | Memphis | A110 Arts, Culture & Humanities | $44.3M | 2 |
| NASHVILLE CONVENTION & VISITORS BUREAU | Nashville | S41 Community Improvement & Capacity Building | $44M | 12 |
| Alpha Omicron Pi Fraternity Inc Group Return | Brentwood | — | $43.9M | 0 |
| JOHNSON UNIVERSITY | Knoxville | B500 Education | $43.7M | 7 |
| GESTALT COMMUNITY SCHOOLS | Memphis | B29 Education | $43.1M | 9 |
| CHATTANOOGA AREA FOOD BANK INC | Chattanooga | K31Z Food, Agriculture & Nutrition | $42.5M | 2 |
| CHRISTIAN COMMUNITY DEVELOPMENT | Memphis | — | $41.5M | 2 |
| MAURY REGIONAL MEDICAL GROUP | Columbia | E320 Health Care | $41.4M | 12 |
| FIRST SOUTH FINANCIAL CREDIT UNION | Bartlett | — | $41.2M | 11 |
| FRANKLIN ROAD ACADEMY INC | Nashville | B24Z Education | $41M | 7 |
| HILLCREST HEALTHCARE COMMUNITIES INC | Knoxville | E910 Health Care | $40.8M | 7 |
| RURAL HEALTH SERVICES CONSORTIUM INC | Rogersville | — | $40.2M | 8 |
| The Works Inc | Memphis | L20 Housing & Shelter | $40.2M | 2 |
| NASHVILLE CARES | Nashville | G81Z Diseases, Disorders & Medical Disciplines | $40.1M | 3 |
| THE COMMUNITY FOUNDATION OF GREATER | Chattanooga | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $40M | 4 |
| KING UNIVERSITY | Bristol | B420 Education | $39.8M | 7 |
| TUSCULUM UNIVERSITY | Greeneville | B420 Education | $39.6M | 6 |
| MONTGOMERY BELL ACADEMY | Nashville | B250 Education | $39.2M | 8 |
| ALIVE HOSPICE INC | Nashville | P740 Human Services | $39.2M | 9 |
| SOUTH CENTRAL HUMAN RESOURCE AGENCY INC | Fayetteville | — | $39.1M | 4 |
| CHATTANOOGA CARES INC DBA | Chattanooga | — | $38.9M | 7 |
| TENNESSEE WESLEYAN UNIVERSITY | Athens | B42Z Education | $38.2M | 10 |
| TREZEVANT EPISCOPAL HOME | Memphis | P750 Human Services | $38M | 7 |
| UNIVERSITY SCHOOL OF NASHVILLE | Nashville | B430 Education | $37.2M | 7 |
| THE HARPETH HALL SCHOOL | Nashville | B200 Education | $37.2M | 8 |
| FEDERAL EXPRESS CORPORATION | Collierville | — | $37.1M | 1 |
| SIGNAL CENTERS INC | Chattanooga | — | $36.8M | 4 |
| VALOR COLLEGIATE ACADEMIES | Nashville | B29 Education | $36.6M | 7 |
| Claiborne Medical Center | Knoxville | E20 Health Care | $36.6M | 10 |
