| CHATTANOOGA CHRISTIAN COMMUNITY FOUNDATION | Chattanooga | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $53.6M | 2 |
| NORTH CENTRAL TELEPHONE COOPERATIVE INC | Lafayette | — | $53.4M | 15 |
| CUMBERLAND HEIGHTS FOUNDATION INC | Nashville | F220 Mental Health & Crisis Intervention | $52.5M | 10 |
| CENTERSTONE OF AMERICA INC | Nashville | F99 Mental Health & Crisis Intervention | $51.9M | 19 |
| PLATEAU ELECTRIC COOPERATIVE | Oneida | — | $51.3M | 19 |
| EAST TENNESSEE HUMAN RESOURCE AGENCY INC | Knoxville | — | $51.2M | 5 |
| MEMPHIS GOODWILL INC | Memphis | P29 Human Services | $51.1M | 2 |
| CHATTANOOGA CARES INC | Chattanooga | — | $51.1M | 10 |
| Smyth County Community Hospital | Johnson City | E220 Health Care | $50.5M | 18 |
| PICKWICK ELECTRIC COOPERATIVE | Selmer | — | $50.3M | 17 |
| Fully Alive Foundation Inc | Knoxville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $50M | 0 |
| Roane County Medical Center | Knoxville | E32 Health Care | $49.9M | 12 |
| RETIREE RESOURCES CORPORATION | Knoxville | M202 Public Safety, Disaster Preparedness & Relief | $49.3M | 7 |
| NASHVILLE CONVENTION & VISITORS BUREAU | Nashville | S41 Community Improvement & Capacity Building | $49.3M | 12 |
| ISHA FOUNDATION INC | McMinnville | B60 Education | $48.7M | 0 |
| CCA of Tennessee LLC Employee Benefit Trust | Nashville | Y43 Mutual & Membership Benefit | $48.6M | 1 |
| ALPHA OMICRON PI FRATERNITY INC GROUP RETURN | Brentwood | — | $48.4M | 1 |
| TENNESSEE VALLEY ELECTRIC COOP | Savannah | — | $48.3M | 8 |
| THE MIKE AND TERESA NACARATO FOUNDATION | Franklin | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $48.3M | 0 |
| Fort Loudoun Medical Center | Knoxville | E22Z Health Care | $47.9M | 11 |
| BAPTIST MEMORIAL HEALTH CARE FOUNDATION INC | Memphis | E110 Health Care | $47.5M | 5 |
| HOOSIER CARE INC | Nashville | — | $47.3M | 8 |
| KNOX AREA RESCUE MINISTRIES INC | Knoxville | L410 Housing & Shelter | $46.9M | 2 |
| SECOND HARVEST FOOD BANK OF EAST TENNESSEE | Maryville | — | $46.6M | 1 |
| UNITED WAY OF MIDDLE TENNESSEE INC | Nashville | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $46.2M | 11 |
| NATIONAL ASSOCIATION OF STATE BOARDS OF ACCOUNTANCY INC | Nashville | — | $46M | 13 |
| BETHEL UNIVERSITY | McKenzie | B43Z Education | $45.9M | 10 |
| TELLICO VILLAGE PROPERTY OWNERS ASSOCATION INC | Loudon | L50 Housing & Shelter | $45M | 6 |
| THE ORANGE GROVE CENTER INC | Chattanooga | P82Z Human Services | $44.8M | 7 |
| BLOOD ASSURANCE INC | Chattanooga | E61Z Health Care | $44.6M | 8 |
| JOHNSON UNIVERSITY | Knoxville | B500 Education | $43.7M | 7 |
| GESTALT COMMUNITY SCHOOLS | Memphis | B29 Education | $43.4M | 8 |
| FRANKLIN ROAD ACADEMY INC | Nashville | B24Z Education | $43M | 8 |
| CHATTANOOGA AREA FOOD BANK INC | Chattanooga | K31Z Food, Agriculture & Nutrition | $42.5M | 2 |
| SIGNAL CENTERS INC | Chattanooga | — | $41.9M | 5 |
| IOTA Community Schools | Memphis | B25 Education | $41.9M | 7 |
| CHRISTIAN COMMUNITY DEVELOPMENT CORPORATION | Memphis | — | $41.5M | 2 |
| MAURY REGIONAL MEDICAL GROUP | Columbia | E320 Health Care | $41.4M | 13 |
| FIRST SOUTH FINANCIAL CREDIT UNION | Bartlett | — | $41.2M | 11 |
| UNIVERSITY OF MEMPHIS FOUNDATION | Memphis | B110 Education | $40.9M | 4 |
| HILLCREST HEALTHCARE COMMUNITIES INC | Knoxville | E910 Health Care | $40.8M | 7 |
| RURAL HEALTH SERVICES CONSORTIUM INC | Rogersville | — | $40.2M | 8 |
| THE COMMUNITY FOUNDATION OF GREATER CHATTANOOGA INC | Chattanooga | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $40M | 4 |
| KING UNIVERSITY | Bristol | B420 Education | $39.8M | 7 |
| MONTGOMERY BELL ACADEMY OF THE UNIVERSITY OF NASHVILLE | Nashville | B250 Education | $39.2M | 8 |
| Memphis Brooks Museum of Art Inc | Memphis | A110 Arts, Culture & Humanities | $39.2M | 6 |
| TUSCULUM UNIVERSITY | Greeneville | B420 Education | $39.2M | 6 |
| ALIVE HOSPICE INC | Nashville | P740 Human Services | $39.2M | 9 |
| SOUTH CENTRAL HUMAN RESOURCE AGENCY INC | Fayetteville | — | $39.1M | 4 |
| TENNESSEE WESLEYAN UNIVERSITY | Athens | B42Z Education | $38.2M | 10 |
