| RAYIM OF HUDSON VALLEY INC | Monroe | P82 Human Services | $162.8M | 7 |
| WSNCHS NORTH INC | Bethpage | E220 Health Care | $162.5M | 10 |
| NIAGARA UNIVERSITY | Niagara University | B430 Education | $161.7M | 8 |
| THE QUEENS BOROUGH PUBLIC LIBRARY | Jamaica | B700 Education | $161.5M | 11 |
| ROME MEMORIAL HOSPITAL | Rome | E22Z Health Care | $160.9M | 10 |
| THE ROXANNE AND HENRY BRANDT FOUNDATION | New York | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $160.6M | 0 |
| CHRISTO AND JEANNE-CLAUDE FOUNDATION | New York | A51 Arts, Culture & Humanities | $160.4M | 2 |
| THE CIVIL SERVICE EMPLOYEES ASSOCIATION INC | Albany | — | $160.3M | 72 |
| ST JOHN FISHER UNIVERSITY | Rochester | B430 Education | $160.2M | 18 |
| DEVELOPMENTAL DISABILITIES INSTITUTE INC | Smithtown | P820 Human Services | $159.8M | 9 |
| WASHINGTON-SARATOGA-WARREN-HAMILTON ESSEX COUNTIES BOCES HEALTH INS CON | Hudson Falls | Y44 Mutual & Membership Benefit | $157.2M | 0 |
| OPEN SOCIETY ACTION FUND INC | New York | — | $157.2M | 5 |
| HUDSON EAST RIVER SYSTEMS LLC | New York | E12 Health Care | $156.9M | 4 |
| NORTHWELL HEALTH FOUNDATION | Westbury | E122 Health Care | $155.8M | 20 |
| THE CHILDREN'S VILLAGE INC | Dobbs Ferry | P730 Human Services | $155.7M | 7 |
| AMERICU CREDIT UNION | Rome | — | $153.3M | 10 |
| The Royal National Theatre | New York | A65 Arts, Culture & Humanities | $152.4M | 8 |
| Hadassah The Women's Zionist Org of America Inc | New York | S810 Community Improvement & Capacity Building | $152.3M | 9 |
| NY44 HEALTH BENEFITS PLAN TRUST | West Seneca | Y44 Mutual & Membership Benefit | $152.3M | 0 |
| ADIRONDACK MEDICAL CENTER | Saranac Lake | E220 Health Care | $152M | 13 |
| AMERICAN ARBITRATION ASSOCIATION INC | New York | I510 Crime & Legal-Related | $151.3M | 14 |
| Westhab Inc | Yonkers | L410 Housing & Shelter | $151.3M | 17 |
| THE CHASE AND STEPHANIE COLEMAN FOUNDATION | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $150.8M | 0 |
| NATIONAL AUDUBON SOCIETY INC | New York | C300 Environment | $149.9M | 11 |
| Mount St Mary's Hospital of Niagara Falls | Buffalo | E220 Health Care | $149.6M | 21 |
| ACTION AGAINST HUNGER - USA | New York | Q300 International, Foreign Affairs & National Security | $149.2M | 11 |
| The Winifred Masterson Burke Rehabilitation Hospital | White Plains | E20 Health Care | $149.2M | 11 |
| MONDELEZ GLOBAL LLC GROUP BENEFIT TRUST I | New York | Y43 Mutual & Membership Benefit | $149.2M | 1 |
| The New York Community Hospital of Brooklyn Inc | Brooklyn | E220 Health Care | $148.2M | 19 |
| PAINTING INDUSTRY INSURANCE FUND | New York | — | $147.9M | 11 |
| HERITAGE CHRISTIAN SERVICES INC | Rochester | P820 Human Services | $147.9M | 10 |
| ONEIDA HEALTH SYSTEMS | Oneida | E220 Health Care | $147M | 13 |
| NIAGARA FALLS MEMORIAL MEDICAL CENTER | Niagara Falls | E220 Health Care | $146.7M | 13 |
| DIVISION 1181 ATU - NEW YORK WELFARE FUND | Valley Stream | — | $146.1M | 6 |
| LVMH MOET HENNESSY LOUIS VUITTON INC AND AFFILIATES VEBA TRUST | New York | — | $145.2M | 0 |
| NEW YORK STATE HEALTH FDN D/B/A NEW YORK HEALTH FOUNDATION | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $145.1M | 1 |
| FRIENDS OF UNITED HATZALAH INC | New York | A70 Arts, Culture & Humanities | $144.9M | 7 |
| Horace Mann School | Bronx | B200 Education | $144.9M | 11 |
| WELFARE FUND OF THE IUOE LOCAL UNIONS 15 15A 15C 15D AFL-CIO | Long Island City | — | $144.9M | 6 |
| THE COOPER UNION FOR THE ADVANCEMENT OF SCIENCE & ART | New York | B420 Education | $144.8M | 15 |
| CAYUGA HOME FOR CHILDREN | Auburn | P730 Human Services | $144.8M | 23 |
| FOUNDERS PLEDGE INC | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $144.6M | 6 |
| UNITED MEDICAL ASSOCIATES PC | Vestal | E32Z Health Care | $143.3M | 23 |
| GUTHRIE CORTLAND MEDICAL CENTER FKA CORTLAND REGIONAL MEDICAL CENTER | Cortland | E220 Health Care | $143.3M | 13 |
| ST JOSEPH'S MEDICAL PC | Syracuse | E300 Health Care | $143.3M | 14 |
| DRUCKENMILLER FOUNDATION | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $143.2M | 0 |
| CLAXTON-HEPBURN MEDICAL CENTER | Ogdensburg | E220 Health Care | $143.1M | 15 |
| COMMUNITY HEALTH AIDE SERVICES INC | Montebello | E92Z Health Care | $142.9M | 10 |
| HILLSIDE CHILDREN'S CENTER | Rochester | P300 Human Services | $142.6M | 7 |
| VERA INSTITUTE OF JUSTICE INC | Brooklyn | I20Z Crime & Legal-Related | $142.3M | 15 |
