| CHINESE-AMERICAN PLANNING COUNCIL HOME ATTENDANT PROGRAM INC | New York | E920 Health Care | $198.8M | 12 |
| Catholic Health System | Buffalo | — | $198.1M | 20 |
| True North Medical Group PC D/B/A Orlin & Cohen Medical Specialists Group | Westbury | E21 Health Care | $196.2M | 10 |
| FOOD BANK FOR NEW YORK CITY | New York | S50 Community Improvement & Capacity Building | $195.8M | 7 |
| Iona University | New Rochelle | B43 Education | $194.2M | 14 |
| DONORSCHOOSEORG | New York | B122 Education | $194M | 10 |
| URBAN HEALTH PLAN INC | Bronx | E32Z Health Care | $193.6M | 8 |
| HOBART AND WILLIAM SMITH COLLEGES | Geneva | B420 Education | $193.4M | 10 |
| ST PETER'S HEALTH PARTNERS | Albany | E21 Health Care | $192.9M | 16 |
| INSTITUTE FOR COMMUNITY LIVING INC | New York | F33 Mental Health & Crisis Intervention | $191.8M | 8 |
| HealthAlliance Hospital | Kingston | E20 Health Care | $189.4M | 18 |
| FRIENDS OF THE ISRAEL DEFENSE FORCES | New York | Q123 International, Foreign Affairs & National Security | $187.5M | 12 |
| KIPP NYC PUBLIC CHARTER SCHOOLS | New York | B29 Education | $186.1M | 11 |
| MOTHER CABRINI HEALTH FOUNDATION INC | New York | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $185.2M | 7 |
| BOWERY RESIDENTS COMMITTEE INC | New York | P200 Human Services | $184.8M | 8 |
| ST MARY'S HEALTHCARE | Amsterdam | E220 Health Care | $184.8M | 17 |
| OSWEGO HOSPITAL | Oswego | E220 Health Care | $184.8M | 10 |
| MAJOR LEAGUE BASEBALL PLAYERS ASSOCIATION | New York | — | $184.7M | 9 |
| DOMINION ENERGY INC COLLECTIVELY BARGAINED VEBA TRUST | New York | — | $184.6M | 1 |
| NEW WATER STREET CORP | New York | — | $180.5M | 8 |
| ROBIN HOOD FOUNDATION | New York | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $180.5M | 13 |
| UNITED MEMORIAL MEDICAL CENTER | Batavia | E22Z Health Care | $180.1M | 14 |
| MOLLOY UNIVERSITY | Rockville Centre | B420 Education | $178.1M | 14 |
| NYC SCHOOL UMBRELLA SERVICES INC | Brooklyn | W40 Public & Societal Benefit | $177.5M | 7 |
| NEW YORK STATE UNITED TEACHERS | Latham | — | $176.8M | 105 |
| 1199SEIU NBF FOR HOME CARE EMPLOYEES | New York | Y43 Mutual & Membership Benefit | $176.4M | 4 |
| HAMASPIK OF KINGS COUNTY INC | Brooklyn | P70I Human Services | $175.3M | 6 |
| ICIRCLE SERVICES OF THE FINGER LAKES INC | Webster | P82 Human Services | $172M | 11 |
| NATIONAL MULTIPLE SCLEROSIS SOCIETY | New York | G500 Diseases, Disorders & Medical Disciplines | $171.8M | 16 |
| LINCOLN CENTER FOR THE PERFORMING ARTS INC | New York | A610 Arts, Culture & Humanities | $171.7M | 12 |
| THE SIMONS FOUNDATION INC | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $171.4M | 11 |
| UPMC CHAUTAUQUA AT WCA | Jamestown | E220 Health Care | $171.3M | 12 |
| THE CENTER FOR DISCOVERY INC | Harris | G200 Diseases, Disorders & Medical Disciplines | $171M | 9 |
| SESAME WORKSHOP | New York | A320 Arts, Culture & Humanities | $170.3M | 13 |
| FAMILY RESIDENCES AND ESSENTIAL ENTERPRISES INC | Old Bethpage | F330 Mental Health & Crisis Intervention | $169.2M | 14 |
| NEO Philanthropy Inc | New York | W99Z Public & Societal Benefit | $168.9M | 12 |
| BROOKLYN PUBLIC LIBRARY | Brooklyn | B71Z Education | $168M | 9 |
| Montefiore New Rochelle Hospital | New Rochelle | E22 Health Care | $167.9M | 10 |
| RF CATALYTIC CAPITAL INC | New York | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $167.6M | 14 |
| AMERICAN FRIENDS OF HEBREW UNIVERSITY INC | New York | Q113 International, Foreign Affairs & National Security | $167.5M | 11 |
| NYSARC INC Nassau County Chapter | Brookville | P820 Human Services | $167.3M | 11 |
| THE INSTITUTE FOR FAMILY HEALTH | New York | E320 Health Care | $167.1M | 17 |
| Le Moyne College | Syracuse | B420 Education | $166.5M | 10 |
| CONCEPTS OF INDEPENDENCE INC | New York | — | $166.4M | 7 |
| THE CHILDREN'S AID SOCIETY | New York | P300 Human Services | $165.5M | 10 |
| UNCOMMON NEW YORK CITY CHARTER SCHOOLS | New York | B29 Education | $165.2M | 15 |
| WOMEN IN NEED INC | New York | P83Z Human Services | $164M | 12 |
| THE FEINSTEIN INSTITUTES FOR MEDICAL RESEARCH | Westbury | H900 Medical Research | $163.3M | 10 |
| CSEA EMPLOYEE BENEFIT FUND | Latham | — | $163.3M | 3 |
| ANTI-DEFAMATION LEAGUE | New York | R600 Civil Rights, Social Action & Advocacy | $162.9M | 12 |
