| HMONG AMERICAN PEACE ACADEMY LTD | Milwaukee | B29 Education | $32.5M | 9 |
| MILWAUKEE REGIONAL MEDICAL CENTER THERMAL SERVICE INC | Milwaukee | E112 Health Care | $32.5M | 1 |
| MILWAUKEE JEWISH FEDERATION INC | Milwaukee | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $32.3M | 10 |
| MMAC COMMUNITY SUPPORT FOUNDATION INC | Milwaukee | S80Z Community Improvement & Capacity Building | $32M | 1 |
| SC JOHNSON & SON INC RETIREE MEDICAL BENEFITS TRUST | Racine | Y43 Mutual & Membership Benefit | $31.7M | 0 |
| ST CAMILLUS HEALTH CENTER INC | Wauwatosa | P750 Human Services | $31.6M | 7 |
| WISCONSIN HEALTH FUND | Milwaukee | — | $31.3M | 10 |
| GREATER WATERTOWN COMMUNITY HEALTH FOUNDATION INC | Watertown | E220 Health Care | $31.1M | 4 |
| WEA INSURANCE CORPORATION | Madison | — | $30.8M | 13 |
| NORTHLAND COLLEGE | Ashland | B420 Education | $30.7M | 6 |
| FORWARD SERVICE CORPORATION | Madison | J200 Employment | $30.5M | 7 |
| THE MILWAUKEE SCIENCE EDUCATION CONSORTIUM INC | Milwaukee | B20 Education | $30.3M | 2 |
| BOYS AND GIRLS CLUB OF GREATER MILWAUKEE INC | Milwaukee | O230 Youth Development | $30.3M | 10 |
| THE LUTHERAN HOME INC | Wauwatosa | E910 Health Care | $30.2M | 8 |
| ASSOCIATION OF EQUIPMENT MANUFACTURERS | Milwaukee | S41 Community Improvement & Capacity Building | $30.1M | 7 |
| ASPIRUS INC | Wausau | E120 Health Care | $30.1M | 28 |
| BAY AREA LUTHERAN HOMES INC | Green Bay | P750 Human Services | $29.9M | 6 |
| Edgerton Hospital and Health Services Inc | Edgerton | E22 Health Care | $29.9M | 7 |
| HOPE CHRISTIAN SCHOOLS INC | Waukesha | — | $29.9M | 6 |
| EAU CLAIRE ENERGY COOPERATIVE | Fall Creek | — | $29.8M | 17 |
| Reiman Foundation Inc | Pewaukee | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $29.8M | 0 |
| FUTURE WISCONSIN HOUSING FUND INC | Middleton | L25 Housing & Shelter | $29.8M | 8 |
| WPSC Master Welfare Benefit Trust | Milwaukee | — | $29.7M | 0 |
| INTEGRATED COMMUNITY SOLUTIONS INC | Green Bay | L200 Housing & Shelter | $29.2M | 4 |
| BURNETT MEDICAL CENTER INC | Grantsburg | E24Z Health Care | $29.2M | 7 |
| CO-OP CREDIT UNION | Black River Falls | — | $29.2M | 15 |
| ST PAUL ELDER SERVICES INC | Kaukauna | — | $29.1M | 9 |
| EDUCATION ANALYTICS INC | Madison | U19 Science & Technology | $29M | 14 |
| THEDACARE MEDICAL CENTER - BERLIN INC | Appleton | E220 Health Care | $29M | 0 |
| ThedaCare Medical Center - Waupaca Inc | Appleton | E22 Health Care | $28.4M | 0 |
| ACADEMY OF EXCELLENCE | Milwaukee | B24 Education | $28.3M | 2 |
| BALL STATE UNIV VEBA VANGUARD INDEX | Milwaukee | — | $28.3M | 1 |
| COMMUNITY ADVOCATES INC | Milwaukee | — | $28.2M | 3 |
| CLARITY CARE INC | Oshkosh | P73 Human Services | $28.2M | 3 |
| DUNN ENERGY COOPERATIVE | Menomonie | — | $28.2M | 19 |
| IMPACT SEVEN INC | Rice Lake | S200 Community Improvement & Capacity Building | $27.7M | 8 |
| ST CROIX ELECTRIC COOPERATIVE | Hammond | — | $27.5M | 17 |
| St Joseph's Hospital of the Hospital Sisters of the Third Order of St Francis | Chippewa Falls | E220 Health Care | $27.3M | 9 |
| CLARK ELECTRIC COOPERATIVE | Greenwood | — | $27M | 13 |
| BOYS & GIRLS CLUB FOX VALLEY INC | Appleton | P300 Human Services | $27M | 1 |
| LUTHERAN HOMES OF OCONOMOWOC INC | Oconomowoc | P750 Human Services | $26.9M | 8 |
| EVERGREEN RETIREMENT COMMUNITY INC | Oshkosh | P750 Human Services | $26.9M | 5 |
| AMERICAN FAMILY INSURANCE DREAMS FOUNDATION INC | Madison | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.9M | 0 |
| BETHANY-ST JOSEPH CORPORATION | La Crosse | — | $26.8M | 9 |
| INDIAN COMMUNITY SCHOOL INC | Franklin | B200 Education | $26.7M | 12 |
| HUNGER TASK FORCE INC | West Milwaukee | — | $26.7M | 6 |
| AMERICAN SOCIETY FOR QUALITY INC | Milwaukee | S030 Community Improvement & Capacity Building | $26.6M | 8 |
| THE YABUKI FAMILY FOUNDATION | Milwaukee | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.3M | 0 |
| SIMPLICITY CREDIT UNION | Marshfield | — | $26.3M | 15 |
| AMES FAMILY LEGACY FOUNDATION | Milwaukee | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.2M | 0 |
