| THE HOWARD YOUNG MEDICAL CENTER INC | Woodruff | E22 Health Care | $31.1M | 24 |
| GREATER WATERTOWN COMMUNITY HEALTH | Watertown | E220 Health Care | $31.1M | 4 |
| The Lynde and Harry Bradley Foundation Inc | Milwaukee | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $31M | 12 |
| WEA INSURANCE CORPORATION | Madison | — | $30.8M | 13 |
| VERSITI INC | Milwaukee | E11 Health Care | $30.8M | 12 |
| FORWARD SERVICE CORPORATION | Madison | J200 Employment | $30.5M | 7 |
| BOYS AND GIRLS CLUB OF GREATER | Milwaukee | O230 Youth Development | $30.3M | 10 |
| THE LUTHERAN HOME INC | Wauwatosa | E910 Health Care | $30.2M | 8 |
| ASSOCIATION OF EQUIPMENT MANUFACTURERS | Milwaukee | S41 Community Improvement & Capacity Building | $30.1M | 7 |
| HEARTLAND CREDIT UNION | Madison | — | $30M | 13 |
| Edgerton Hospital and Health | Edgerton | E22 Health Care | $29.9M | 7 |
| EAU CLAIRE ENERGY COOPERATIVE | Fall Creek | — | $29.8M | 17 |
| GREATER GREEN BAY COMMUNITY | Green Bay | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $29.8M | 4 |
| ORC Industries Inc | La Crosse | J330 Employment | $29.7M | 3 |
| WPSC Master Welfare Benefit Trust | Milwaukee | — | $29.7M | 0 |
| UNITY LIMITED PARTNERSHIP | De Pere | P74 Human Services | $29.6M | 13 |
| INTEGRATED COMMUNITY SOLUTIONS INC | Green Bay | L200 Housing & Shelter | $29.2M | 4 |
| BURNETT MEDICAL CENTER INC | Grantsburg | E24Z Health Care | $29.2M | 7 |
| ST PAUL ELDER SERVICES INC | Kaukauna | — | $29.1M | 9 |
| EDUCATION ANALYTICS INC | Madison | U19 Science & Technology | $29M | 14 |
| HOPE CHRISTIAN SCHOOLS INC | Waukesha | — | $29M | 6 |
| OVERTURE CENTER FOUNDATION INC | Madison | A11 Arts, Culture & Humanities | $28.9M | 8 |
| OSHKOSH AREA COMMUNITY FOUNDATION | Oshkosh | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $28.8M | 3 |
| DUNN ENERGY COOPERATIVE | Menomonie | — | $28.7M | 17 |
| ACADEMY OF EXCELLENCE | Milwaukee | B24 Education | $28.3M | 2 |
| COMMUNITY ADVOCATES INC | Milwaukee | — | $28.2M | 3 |
| CLARITY CARE INC | Oshkosh | P73 Human Services | $28.2M | 3 |
| FUTURE WISCONSIN HOUSING FUND INC | Middleton | L25 Housing & Shelter | $28M | 6 |
| ASPIRUS MERRILL HOSPITAL & CLINICS INC | Merrill | E20 Health Care | $27.4M | 24 |
| ST CROIX ELECTRIC COOPERATIVE | Hammond | — | $27.2M | 18 |
| NEXT DOOR FOUNDATION INC | Milwaukee | N122 Recreation & Sports | $27M | 4 |
| KOHLER CREDIT UNION | Sheboygan | — | $27M | 10 |
| BOYS & GIRLS CLUB FOX VALLEY INC | Appleton | P300 Human Services | $27M | 1 |
| LUTHERAN HOMES OF OCONOMOWOC INC | Oconomowoc | P750 Human Services | $26.9M | 8 |
| AMERICAN FAMILY INSURANCE DREAMS | Madison | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.9M | 0 |
| INDIAN COMMUNITY SCHOOL INC | Franklin | B200 Education | $26.8M | 11 |
| BETHANY-ST JOSEPH CORPORATION | La Crosse | — | $26.8M | 9 |
| THE UWM FOUNDATION INC | Milwaukee | B430 Education | $26.7M | 5 |
| HUNGER TASK FORCE INC | West Milwaukee | — | $26.7M | 6 |
| Historic Haymarket Milwaukee Inc | Milwaukee | A50 Arts, Culture & Humanities | $26.6M | 0 |
| AMERICAN SOCIETY FOR QUALITY INC | Milwaukee | S030 Community Improvement & Capacity Building | $26.6M | 8 |
| NORTHLAND COLLEGE | Ashland | B420 Education | $26.6M | 7 |
| THE YABUKI FAMILY FOUNDATION | Milwaukee | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.3M | 0 |
| SIMPLICITY CREDIT UNION | Marshfield | — | $26.3M | 15 |
| AMES FAMILY LEGACY FOUNDATION | Milwaukee | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.2M | 0 |
| NATIONAL REGENCY OF NEW BERLIN INC | Waukesha | P750 Human Services | $26.1M | 8 |
| CLARK ELECTRIC COOPERATIVE | Greenwood | — | $25.9M | 13 |
| ST COLETTA OF WISCONSIN INC | Jefferson | B60 Education | $25.4M | 4 |
| HOME HEALTH UNITED INC | Madison | E92Z Health Care | $25.2M | 7 |
| Jackson Electric Cooperative | Black River Falls | — | $25.2M | 15 |
