| NATIONAL REGENCY OF NEW BERLIN INC | Waukesha | P750 Human Services | $26.1M | 8 |
| THE ARGOSY FOUNDATION | Milwaukee | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.1M | 1 |
| 1923 FUND | Milwaukee | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $25.8M | 2 |
| MILWAUKEE CATHOLIC HOME INC | Milwaukee | P750 Human Services | $25.6M | 4 |
| THE HILLS AT CORTLAND RIDGE | Watertown | L220 Housing & Shelter | $25.6M | 0 |
| ST COLETTA OF WISCONSIN INC | Jefferson | B60 Education | $25.4M | 4 |
| NEXT DOOR FOUNDATION INC | Milwaukee | N122 Recreation & Sports | $25.3M | 3 |
| HOME HEALTH UNITED INC | Madison | E92Z Health Care | $25.2M | 7 |
| MILWAUKEE HEALTH SERVICES INC | Milwaukee | E320 Health Care | $25M | 9 |
| AURORA HEALTH CARE FOUNDATION INC | Milwaukee | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $24.4M | 24 |
| JACKSON ELECTRIC COOPERATIVE | Black River Falls | — | $24.2M | 16 |
| LA CASA DE ESPERANZA INC | Waukesha | — | $24.2M | 7 |
| OUTREACH COMMUNITY HEALTH CENTERS INC | Milwaukee | E30Z Health Care | $24.1M | 8 |
| VERNON ELECTRIC COOPERATIVE | Westby | — | $24.1M | 17 |
| FAMILY SERVICES OF NORTHEAST WI INC | Green Bay | P40Z Human Services | $24M | 8 |
| JEWISH HOME AND CARE CENTER INC | Milwaukee | E91Z Health Care | $24M | 7 |
| University Research Park Incorporated | Madison | B110 Education | $24M | 5 |
| INDEPENDENCE FIRST INC | Milwaukee | P80Z Human Services | $24M | 8 |
| ASPIRUS STANLEY HOSPITAL & CLINICS INC | Stanley | E22 Health Care | $24M | 25 |
| SAN CAMILLO INC | Wauwatosa | P750 Human Services | $23.6M | 6 |
| ASPIRUS VNA HOME HEALTH INC | Wausau | — | $23.5M | 24 |
| WISCONSIN MASONS' HEALTH CARE FUND | Madison | — | $23.5M | 7 |
| LAC COURTE OREILLES OJIBWE SCHOOL | Hayward | B99Z Education | $23.5M | 2 |
| FIRST COMMUNITY CREDIT UNION OF BELOIT | Beloit | — | $23.3M | 7 |
| Joseph and Vera Zilber Family Foundation Inc | Milwaukee | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $23.3M | 8 |
| PCM CREDIT UNION | Green Bay | — | $23.3M | 8 |
| CHIPPEWA VALLEY HOSPITAL AND OAKVIEW CARE CENTER INC | Durand | E22 Health Care | $23M | 21 |
| WAICU BENEFITS CONSORTIUM INC | Madison | Y43 Mutual & Membership Benefit | $22.9M | 0 |
| ENERGY SERVICES INC | Fitchburg | L820 Housing & Shelter | $22.9M | 7 |
| CAPITOL LAKES INC | Madison | P750 Human Services | $22.8M | 6 |
| PIERCE-PEPIN COOPERATIVE SERVICES | Ellsworth | — | $22.6M | 18 |
| DWIGHT AND LINDA DAVIS FOUNDATION | Wausau | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $22.5M | 2 |
| WISCONSIN BANKERS ASSOCIATION INSURANCE TRUST FUND | Sun Prairie | — | $22.4M | 1 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF GREATER WAUKESHA COUNTY INC | New Berlin | N30 Recreation & Sports | $22.3M | 4 |
| CINTI BELL ACTIVE MEDICAL CARE | Milwaukee | — | $22.3M | 1 |
| CHILEDA INSTITUTE INC | La Crosse | — | $22.2M | 2 |
| COMMUNITY BLOOD CENTER INC | Appleton | E61Z Health Care | $21.9M | 11 |
| SEEDS OF HEALTH INC | Milwaukee | — | $21.9M | 8 |
| JOURNEY MENTAL HEALTH CENTER INC | Madison | F30Z Mental Health & Crisis Intervention | $21.8M | 10 |
| TRI-COUNTY COMMUNICATIONS COOPERATIVE INC | Strum | — | $21.8M | 12 |
| INNOVATIVE SERVICES INC | Green Bay | P20 Human Services | $21.8M | 7 |
| PROSPERA CREDIT UNION | Appleton | — | $21.7M | 11 |
| MILWAUKEE ART MUSEUM INC | Milwaukee | A510 Arts, Culture & Humanities | $21.6M | 10 |
| GRACE LUTHERAN FOUNDATION INC | Eau Claire | E910 Health Care | $21.5M | 5 |
| LA CLINICA DE LOS CAMPESINOS INC | Wautoma | — | $21.4M | 7 |
| ACADEMY OF BASIC EDUCATION INC D/B/A BROOKFIELD ACADEMY | Brookfield | B24Z Education | $21.4M | 8 |
| POHLAD COMPANIES VEBA | Milwaukee | Y20 Mutual & Membership Benefit | $21.4M | 1 |
| OAKWOOD VILLAGE PRAIRIE RIDGE HOMES INC | Madison | L22 Housing & Shelter | $21.3M | 8 |
| EVIDENT CHANGE | Madison | I050 Crime & Legal-Related | $21.3M | 14 |
| Peoples Energy Corporation Life & Health Benefit Trust | Milwaukee | — | $21.3M | 0 |
