| ROCKY MOUNTAIN INSTITUTE | Boulder | — | $139.4M | 14 |
| BOHEMIAN FOUNDATION | Fort Collins | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $139.2M | 2 |
| ROCKY MOUNTAIN UFCW UNIONS & EMPLOYERS | Arvada | — | $138.1M | 4 |
| ARC THRIFT STORES INC | Lakewood | P820 Human Services | $137.6M | 12 |
| YAMPA VALLEY MEDICAL CENTER | Steamboat Springs | E220 Health Care | $136.4M | 12 |
| MOUNTAIN VIEW ELECTRIC ASSOCIATION | Limon | — | $131.4M | 18 |
| LUTHERAN HOSPITAL ASSOC OF THE SAN LUIS | Alamosa | E22 Health Care | $124.7M | 24 |
| DANIELS FUND | Denver | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $123M | 18 |
| CATENA FOUNDATION | Carbondale | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $121.9M | 1 |
| Children's Hospital Colorado Health System | Aurora | E19 Health Care | $121.8M | 3 |
| CREDIT UNIONS CHARTERED IN COLORADO | Denver | — | $121.3M | 19 |
| LA PLATA ELECTRIC ASSOCIATION INC | Durango | — | $118.2M | 25 |
| Colorado Coalition for the Homeless | Denver | P850 Human Services | $116.9M | 16 |
| INTERNATIONAL MEDICAL RELIEF | Loveland | E99 Health Care | $111.6M | 2 |
| SALUD FAMILY HEALTH | Fort Lupton | E20Z Health Care | $111.4M | 11 |
| PEAK VISTA COMMUNITY HEALTH CENTERS | Colorado Springs | E32Z Health Care | $111.4M | 9 |
| THE DENVER FOUNDATION | Denver | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $108.7M | 10 |
| DENVER SCHOOL OF SCIENCE AND TECHNOLOGY INC | Denver | B40 Education | $108.1M | 12 |
| Rocky Mountain Health Care Services | Colorado Springs | — | $106.4M | 8 |
| THE COLORADO HEALTH FOUNDATION | Denver | E21 Health Care | $103.6M | 5 |
| MERCY HOUSING INC | Denver | L200 Housing & Shelter | $100.5M | 26 |
| SIGNAL BEHAVIORAL HEALTH NETWORK | Greenwood Village | E99Z Health Care | $100.3M | 7 |
| Longmont United Hospital | Longmont | E220 Health Care | $98.6M | 12 |
| COLORADO STATE UNIVERSITY FOUNDATION | Fort Collins | B110 Education | $96.9M | 7 |
| WIND CREST INC | Highlands Ranch | P75 Human Services | $96.3M | 7 |
| Hexagon Foundation | Colorado Springs | X20 Religion-Related | $95.9M | 3 |
| CABLE TELEVISION LABORATORIES INC | Louisville | — | $95.3M | 15 |
| Strive Preparatory Schools | Denver | B29 Education | $92.7M | 12 |
| COLORADO GIVES FOUNDATION | Arvada | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $92.6M | 7 |
| BENEVOLENT HEALTHCARE FOUNDATION | Centennial | G19 Diseases, Disorders & Medical Disciplines | $90.6M | 5 |
| LOWER VALLEY HOSPITAL ASSOCIATION | Fruita | E21Z Health Care | $87.9M | 11 |
| PREMIER MEMBERS CREDIT UNION | Broomfield | — | $87.7M | 36 |
| ROCKY MOUNTAIN HUMAN SERVICES | Denver | P700 Human Services | $84.5M | 9 |
| WATERSTONE SUPPORTING ORGANIZATION | Colorado Springs | — | $82.6M | 2 |
| JEFFERSON CENTER FOR MENTAL HEALTH | Wheat Ridge | F320 Mental Health & Crisis Intervention | $80.9M | 9 |
| SOUTHWEST HEALTH SYSTEM INC | Cortez | E21Z Health Care | $79.6M | 8 |
| Delta Montrose Electric Association | Montrose | — | $79M | 22 |
| YAMPA VALLEY ELECTRIC ASSOCIATION | Steamboat Springs | — | $78.6M | 23 |
| NORTHEAST HEALTH PARTNERS LLC | Greeley | E70 Health Care | $78.5M | 3 |
| MOUNTAIN WEST CONFERENCE | Colorado Springs | N70 Recreation & Sports | $78.2M | 19 |
| JOINT RESTORATION FOUNDATION INC | Centennial | E65 Health Care | $77.1M | 8 |
| Children's Hospital Colorado Foundation | Aurora | E110 Health Care | $76.1M | 17 |
| COLORADO EARLY COLLEGES | Fort Collins | B25 Education | $75.8M | 6 |
| Summitstone Health Partners | Fort Collins | F30 Mental Health & Crisis Intervention | $75.8M | 10 |
| ENERGY OUTREACH COLORADO | Denver | N20 Recreation & Sports | $74.8M | 10 |
| CHRISTIAN LIVING NEIGHBORHOODS | Englewood | X20 Religion-Related | $73M | 8 |
| ARAPAHOE MENTAL HEALTH CENTER | Englewood | F30Z Mental Health & Crisis Intervention | $72.8M | 11 |
| DEVELOPMENTAL PATHWAYS INC | Aurora | P700 Human Services | $70.1M | 11 |
| THE COMMUNITY FIRM | Denver | I80 Crime & Legal-Related | $69.4M | 5 |
| YMCA OF THE ROCKIES | Estes Park | P270 Human Services | $69.2M | 15 |
