| AFRICAN PARKS FOUNDATION OF AMERICA | Washington | N113 Recreation & Sports | $51.6M | 6 |
| TRINITY COLLEGE | Washington | B420 Education | $51.4M | 8 |
| AMERICAN SOCIETY FOR MICROBIOLOGY | Washington | U030 Science & Technology | $51.3M | 14 |
| AMERICAN HEALTH CARE ASSOCIATION | Washington | — | $51.2M | 18 |
| ZERO TO THREE NATIONAL CENTER FOR INFANTS TODDLERS AND FAMILIES | Washington | P050 Human Services | $50.8M | 13 |
| THINK OF US | Washington | P20 Human Services | $50.7M | 7 |
| OPEN TECHNOLOGY FUND | Washington | A05 Arts, Culture & Humanities | $50.6M | 9 |
| AMIDEAST | Washington | Q200 International, Foreign Affairs & National Security | $50.6M | 14 |
| HOME BUILDERS INSTITUTE | Washington | J220 Employment | $50.4M | 15 |
| US GREEN BUILDING COUNCIL INC | Washington | C03 Environment | $50.3M | 9 |
| WASHINGTON INTERNATIONAL SCHOOL | Washington | B24Z Education | $50.2M | 9 |
| HUMAN RIGHTS CAMPAIGN INC | Washington | R26 Civil Rights, Social Action & Advocacy | $50.1M | 18 |
| WHITMAN-WALKER CLINIC INC | Washington | G810 Diseases, Disorders & Medical Disciplines | $49.9M | 9 |
| AMERICAN CANCER SOCIETY CANCER ACTION NETWORK INC | Washington | H01 Medical Research | $49.8M | 12 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF METROPOLITAN WASHINGTON | Washington | P270 Human Services | $49.2M | 13 |
| OCEAN CONSERVANCY | Washington | C320 Environment | $49.2M | 12 |
| RESULTS FOR DEVELOPMENT INSTITUTE INC | Washington | Q33 International, Foreign Affairs & National Security | $49.1M | 11 |
| FOUNDATION FOR FOOD AND AGRICULTURE RESEARCH | Washington | K99 Food, Agriculture & Nutrition | $49.1M | 9 |
| UNITED PLANNING ORGANIZATION | Washington | P200 Human Services | $48.7M | 7 |
| ADVANCED MEDICAL TECHNOLOGY ASSOCIATION | Washington | — | $48.6M | 11 |
| AFL-CIO HEALTH & WELFARE TRUST | Washington | Y43 Mutual & Membership Benefit | $48.5M | 6 |
| DISTRICT OF COLUMBIA INTERNATIONAL SCHOOL | Washington | B29 Education | $48.4M | 7 |
| NATIONAL MINING ASSOCIATION | Washington | — | $48.1M | 8 |
| AMERICAN TRUCKING ASSOCIATIONS INC | Washington | — | $48.1M | 9 |
| Great Barrier Reef Foundation USA Inc | Washington | C30 Environment | $47.8M | 0 |
| NATIONAL PARKS CONSERVATION ASSOCIATION | Washington | C600 Environment | $47.7M | 12 |
| MERIDIAN INTERNATIONAL CENTER | Washington | Q210 International, Foreign Affairs & National Security | $47.6M | 11 |
| National Postal Mail Handlers Union | Washington | — | $47.6M | 22 |
| AMERICAN EMBASSY SCHOOL | Washington Dc | B24Z Education | $47.6M | 6 |
| TOBACCO-FREE KIDS ACTION FUND | Washington | — | $47.4M | 14 |
| Pew Research Center | Washington | V05 Social Science | $46.7M | 12 |
| NATIONAL CABLE SATELLITE CORPORATION | Washington | A300 Arts, Culture & Humanities | $46.3M | 9 |
| NATIONAL ASSOCIATION OF CHILDREN'S HOSPITALS AND RELATED INSTITUTIONS INC | Washington | — | $46.2M | 12 |
| WORKING AMERICA EDUCATION FUND | Washington | B01 Education | $46M | 1 |
| THE FREEDOM FORUM INC | Washington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $46M | 16 |
| WORKING AMERICA | Washington | — | $45.8M | 10 |
| THE CENTER FOR EMPLOYMENT SECURITY EDUCATION AND RESEARCH INC | Washington | V37 Social Science | $45.8M | 10 |
| AIR TRANSPORT ASSOCIATION OF AMERICA INC | Washington | — | $45.4M | 11 |
| B'NAI B'RITH YOUTH ORGANIZATION | Washington | O55 Youth Development | $44.8M | 9 |
| NATIONAL ELECTRICAL CONTRACTORS ASSOCIATION INC | Washington | — | $44.1M | 12 |
| CENTER CITY PUBLIC CHARTER SCHOOLS | Washington | B29 Education | $43.8M | 6 |
| EHEALTH AFRICA | Washington | E60 Health Care | $43.8M | 8 |
| Global Impact Social Welfare Fund | Washington | W01 Public & Societal Benefit | $43.6M | 0 |
| CENTER FOR COMMUNITY CHANGE | Washington | S99Z Community Improvement & Capacity Building | $43.6M | 10 |
| SOME INC | Washington | — | $43.4M | 11 |
| EARTHSCOPE CONSORTIUM | Washington | M05 Public Safety, Disaster Preparedness & Relief | $43.1M | 12 |
| CENTER FOR DISASTER PHILANTHROPY INC | Washington | M05 Public Safety, Disaster Preparedness & Relief | $42.9M | 7 |
| American Society of Nephrology | Washington | — | $42.9M | 11 |
| REPRODUCTIVE FREEDOM FOR ALL FOUNDATION | Washington | — | $42.5M | 11 |
| FINCA INTERNATIONAL INC | Washington | Q123 International, Foreign Affairs & National Security | $42.5M | 11 |
