| YOUNG WOMEN'S CHRISTIAN ASSOCIATION OF METROPOLITAN ST LOUIS | Saint Louis | — | $33.9M | 8 |
| COMMUNITY TREATMENT INC | Festus | — | $33.7M | 6 |
| ASCENSION CALUMET HOSPITAL INC | St Louis | — | $33.6M | 12 |
| CHRIST IN YOUTH INC | Joplin | — | $33.6M | 0 |
| PERRY COUNTY HEALTH SYSTEM | Perryville | E220 Health Care | $33.6M | 10 |
| SAMUEL U RODGERS HEALTH CENTER INC | Kansas City | E32Z Health Care | $33.6M | 7 |
| Farmers Electric Cooperative Inc | Chillicothe | — | $33.4M | 15 |
| The Whole Person Inc | Kansas City | — | $33.3M | 2 |
| Supported Living and Employment Services Inc | Springfield | P20 Human Services | $33.2M | 8 |
| UMKC FOUNDATION | Kansas City | B11 Education | $33.1M | 5 |
| Cox Barton County Hospital | Lamar | E22 Health Care | $33M | 11 |
| St Vincent Randolph Hospital Inc | St Louis | E220 Health Care | $32.8M | 15 |
| M & A ELECTRIC POWER COOP | Poplar Bluff | — | $32.7M | 17 |
| ST LOUIS PAINTERS WELFARE PLAN | Maryland Heights | — | $32.5M | 0 |
| JOHNNY MORRIS' WONDERS OF WILDLIFE FOUNDATION | Springfield | A50 Arts, Culture & Humanities | $32.4M | 5 |
| WILLOW HEALTH CARE INC | Willow Springs | E910 Health Care | $32.3M | 7 |
| THE GLOBAL ORPHAN PROJECT INC | Kansas City | T90 Philanthropy, Voluntarism & Grantmaking Foundations | $32.3M | 10 |
| THE AMERICAN THEATRE GUILD | Kansas City | A65 Arts, Culture & Humanities | $32.3M | 6 |
| NORTHEAST MISSOURI HEALTH COUNCIL INC | Kirksville | — | $32M | 11 |
| ASCENSION VIA CHRISTI HEALTH INC | St Louis | E210 Health Care | $32M | 13 |
| AREA RESOURCES FOR COMMUNITY & HUMAN SERVICES | St Louis | P80 Human Services | $31.9M | 7 |
| Children International | Kansas City | Q330 International, Foreign Affairs & National Security | $31.7M | 14 |
| Macon Electric Cooperative | Macon | — | $31.3M | 18 |
| CHESS CLUB AND SCHOLASTIC CENTER OF ST LOUIS | St Louis | N31 Recreation & Sports | $31.2M | 8 |
| VOLUNTARY INTERDISTRICT CHOICE CORPORATION | Saint Louis | B120 Education | $31.1M | 1 |
| Alexian Brothers Hospital Network | St Louis | E210 Health Care | $30.9M | 7 |
| INFUZE CREDIT UNION | Waynesville | — | $30.9M | 9 |
| DELTA AREA ECONOMIC OPPORTUNITY CORPORATION | Sikeston | — | $30.5M | 3 |
| CONCORDIA DISABILITY AND SURVIVOR PLAN | St Louis | — | $30.5M | 0 |
| HEROES CARE INC | High Ridge | W30 Public & Societal Benefit | $30.4M | 2 |
| WESTMINSTER COLLEGE | Fulton | B420 Education | $30.2M | 7 |
| Marion and Henry Bloch Family Foundation | Kansas City | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $30.2M | 1 |
| ST VINCENT WILLIAMSPORT HOSPITAL INC | St Louis | — | $30M | 15 |
| WATERORG | Kansas City | Q300 International, Foreign Affairs & National Security | $29.8M | 10 |
| ST LOUIS ART MUSEUM FOUNDATION | St Louis | A110 Arts, Culture & Humanities | $29.7M | 7 |
| The American Optometric Association | St Louis | — | $29.6M | 23 |
| BOARD OF TRUSTEES OF IBEW VACATION FUND | St Louis | — | $29.5M | 0 |
| RIVER REGION CREDIT UNION | Jefferson City | — | $29.5M | 12 |
| Consolidated Electric Cooperative | Mexico | — | $29.4M | 17 |
| SUNNYHILL INC | Fenton | P73Z Human Services | $29.4M | 7 |
| ASCENSION HEALTH SENIOR CARE | St Louis | E210 Health Care | $29.1M | 17 |
| CLARK COMMUNITY MENTAL HEALTH CENTER | Pierce City | E20Z Health Care | $29.1M | 9 |
| PI BETA PHI FRATERNITY HOUSING CORPORATION | Town Country | N50 Recreation & Sports | $28.8M | 2 |
| Sac Osage Electric Cooperative Inc | El Dorado Springs | — | $28.8M | 15 |
| JASPER COUNTY SHELTERED FACILITIES ASSOCIATION INC | Joplin | — | $28.7M | 2 |
| BOARD OF TRUSTEES IBEW-NECA HOLIDAY TRUST FUND - LOCAL 1 | St Louis | — | $28.6M | 0 |
| Family Care Health Centers | St Louis | E320 Health Care | $28.6M | 14 |
| BARRY ELECTRIC COOPERATIVE INC | Cassville | — | $28.6M | 16 |
| PEMISCOT-DUNKLIN ELECTRIC COOPERATIVE INC | Kennett | — | $28.4M | 16 |
| Northwest Medical Center Association Inc | St Joseph | — | $28.4M | 13 |
