| Saint Lukes Hospital of Trenton | Trenton | E21 Health Care | $39.4M | 14 |
| PEMBROKE HILL SCHOOL | Kansas City | B200 Education | $39.2M | 8 |
| Ozarks Area Community Action Corporation | Springfield | P20 Human Services | $39.1M | 1 |
| MARK TWAIN ASSOCIATION FOR MENTAL HEALTH | Hannibal | F32Z Mental Health & Crisis Intervention | $38.9M | 4 |
| LIFE UNLIMITED INC | North Kansas City | P83Z Human Services | $38.8M | 7 |
| OPERATION FOOD SEARCH INC | St Louis | K31Z Food, Agriculture & Nutrition | $38.8M | 4 |
| WILLIAM WOODS UNIVERSITY | Fulton | B430 Education | $38.7M | 3 |
| Owasso Medical Facility Inc | St Louis | E22 Health Care | $38.7M | 6 |
| KANSAS CITY ART INSTITUTE | Kansas City | B400 Education | $38.4M | 7 |
| Kansas City Hospice Inc | Kansas City | P740 Human Services | $38.4M | 9 |
| WILLOW HEALTH CARE INC | Willow Springs | E910 Health Care | $37.9M | 6 |
| Kanakuk Ministries | Branson | X21 Religion-Related | $37.8M | 8 |
| AMERICAN SOYBEAN ASSOCIATION | St Louis | — | $37.3M | 7 |
| st louis community credit union | St Louis | — | $37.3M | 5 |
| MUNICIPAL THEATRE ASSOCIATION OF ST | St Louis | A650 Arts, Culture & Humanities | $37.2M | 9 |
| FREEMAN NEOSHO HOSPITAL INC | Neosho | E22 Health Care | $37M | 10 |
| Central Missouri Electric | Sedalia | — | $36.7M | 17 |
| OATS INC | Columbia | P520 Human Services | $36.4M | 9 |
| THE NELSON GALLERY FOUNDATION | Kansas City | A510 Arts, Culture & Humanities | $36.2M | 6 |
| INTERNATIONAL ASSOCIATION OF SHEET METAL | Saint Louis | — | $36.1M | 5 |
| DELL CHILDREN'S FOUNDATION FKA CMC FOUNDATION OF CENTRAL TEXAS | St Louis | — | $36M | 4 |
| YOUTH IN NEED | St Charles | P320 Human Services | $35.8M | 6 |
| United Way of Greater Kansas City Inc | Kansas City | — | $35.6M | 7 |
| AMEREN MANAGEMENT HEALTH TRUST | Saint Louis | — | $35.5M | 1 |
| CARESTL HEALTH | St Louis | — | $35.5M | 17 |
| MISSOURI STATE UNIVERSITY FOUNDATION | Springfield | B110 Education | $35.5M | 3 |
| GUADALUPE EDUCATIONAL SYSTEM INC | Kansas City | B29 Education | $35.3M | 3 |
| MISSOURI HISTORICAL SOCIETY | St Louis | A540 Arts, Culture & Humanities | $35.1M | 8 |
| OZARK TRI-COUNTY HEALTH CARE CONSORTIUM | Neosho | L80Z Housing & Shelter | $34.7M | 12 |
| PATHWAYS PSYCHIATRIC HOSPITAL INC | Windsor | F31 Mental Health & Crisis Intervention | $34.7M | 11 |
| WEST COMMUNITY CREDIT UNION | Ofallon | — | $34.6M | 12 |
| FRONTIER SCHOOLS INC | Kansas City | B29 Education | $34.6M | 2 |
| West Central Electric Cooperative Inc | Higginsville | — | $34.5M | 16 |
| OZARK BORDER ELECTRIC COOPERATIVE INC | Poplar Bluff | — | $34.4M | 21 |
| JACKSON COUNTY COMMUNITY CHILDREN'S | Kansas City | P30 Human Services | $34.4M | 3 |
| WILLIAM T KEMPER FOUNDATION | Kansas City | — | $34.3M | 2 |
| WHEATON FRANCISCAN HEALTHCARE - SOUTHEAST WISCONSIN INC | St Louis | E21 Health Care | $34.3M | 9 |
| COMMUNITY TREATMENT INC | Festus | — | $33.7M | 6 |
| CHRIST IN YOUTH INC | Joplin | — | $33.6M | 0 |
| PERRY COUNTY HEALTH SYSTEM | Perryville | E220 Health Care | $33.6M | 10 |
| SAMUEL U RODGERS HEALTH CENTER INC | Kansas City | E32Z Health Care | $33.6M | 7 |
| VIA CHRISTI HEALTHCARE OUTREACH PROGRAM FOR ELDERS INC | St Louis | — | $33.5M | 8 |
| Farmers Electric Cooperative Inc | Chillicothe | — | $33.4M | 15 |
| Missouri Credit Union | Columbia | — | $33.4M | 9 |
| NEIGHBORS CREDIT UNION | St Louis | — | $33.3M | 14 |
| The Whole Person Inc | Kansas City | — | $33.3M | 2 |
| Mosaic Health System | St Joseph | E12 Health Care | $33.2M | 26 |
| ASCENSION CALUMET HOSPITAL INC | St Louis | — | $33.1M | 12 |
| UMKC FOUNDATION | Kansas City | B11 Education | $33.1M | 5 |
| Cox Barton County Hospital | Lamar | E22 Health Care | $33M | 11 |
