| CRAWFORD ELECTRIC COOPERATIVE | Bourbon | — | $41.5M | 15 |
| INTERNATIONAL INSTITUTE OF METROPOLITAN ST LOUIS | St Louis | P84Z Human Services | $41.3M | 6 |
| PEMBROKE HILL SCHOOL | Kansas City | B200 Education | $41.2M | 7 |
| CULVER-STOCKTON COLLEGE | Canton | B420 Education | $40.3M | 8 |
| Ascension Borgess Allegan Hospital | St Louis | E220 Health Care | $40.2M | 11 |
| KAUFFMAN CENTER FOR THE PERFORMING ARTS | Kansas City | A61 Arts, Culture & Humanities | $40.2M | 6 |
| EMMAUS HOMES INC | St Charles | — | $40.1M | 9 |
| OATS INC | Columbia | P520 Human Services | $40M | 9 |
| SOUTHWEST BAPTIST UNIVERSITY | Bolivar | B430 Education | $39.8M | 8 |
| Saint Lukes Hospital of Trenton | Trenton | E21 Health Care | $39.4M | 14 |
| MARK TWAIN ASSOCIATION FOR MENTAL HEALTH MARK TWAIN BEHAVIORAL HEALTH | Hannibal | F32Z Mental Health & Crisis Intervention | $38.9M | 4 |
| LIFE UNLIMITED INC | North Kansas City | P83Z Human Services | $38.8M | 7 |
| OPERATION FOOD SEARCH INC | St Louis | K31Z Food, Agriculture & Nutrition | $38.8M | 4 |
| WILLIAM WOODS UNIVERSITY | Fulton | B430 Education | $38.7M | 3 |
| Kansas City Hospice Inc | Kansas City | P740 Human Services | $38.4M | 9 |
| West Central Electric Cooperative Inc | Higginsville | — | $38.2M | 16 |
| Kanakuk Ministries | Branson | X21 Religion-Related | $37.8M | 8 |
| AMERICAN SOYBEAN ASSOCIATION | St Louis | — | $37.3M | 7 |
| MUNICIPAL THEATRE ASSOCIATION OF ST LOUIS | St Louis | A650 Arts, Culture & Humanities | $37.2M | 9 |
| FREEMAN NEOSHO HOSPITAL INC | Neosho | E22 Health Care | $37M | 10 |
| Central Missouri Electric Cooperative Inc | Sedalia | — | $36.7M | 17 |
| ALEXIAN BROTHERS COMMUNITY SERVICES | St Louis | — | $36.7M | 12 |
| St John Providence | St Louis | E210 Health Care | $36.6M | 13 |
| st louis community credit union | St Louis | — | $36.5M | 5 |
| PLACES FOR PEOPLE INCORPORATED | St Louis | F34Z Mental Health & Crisis Intervention | $36.5M | 9 |
| NORTHWEST HEALTH SERVICES INC | Mound City | — | $36.3M | 8 |
| THE NELSON GALLERY FOUNDATION | Kansas City | A510 Arts, Culture & Humanities | $36.2M | 6 |
| MIDWAY USA FOUNDATION INC | Columbia | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $36.2M | 4 |
| INTERNATIONAL ASSOCIATION OF SHEET METAL AIR RAIL AND TRANSPORTION WORKER | Saint Louis | — | $36.1M | 5 |
| CENTER FOR DEVELOPMENTALLY DISABLED | Kansas City | L210 Housing & Shelter | $36M | 3 |
| VIA CHRISTI HEALTHCARE OUTREACH PROGRAM FOR ELDERS INC | St Louis | — | $36M | 12 |
| NEIGHBORS CREDIT UNION | St Louis | — | $36M | 12 |
| MERCY HOSPITAL AURORA | Aurora | E200 Health Care | $35.9M | 14 |
| SOUTH CENTRAL MISSOURI COMMUNITY HEALTH CENTER | Rolla | E30 Health Care | $35.9M | 11 |
| YOUTH IN NEED | St Charles | P320 Human Services | $35.8M | 6 |
| Ozarks Area Community Action Corporation | Springfield | P20 Human Services | $35.8M | 1 |
| United Way of Greater Kansas City Inc | Kansas City | — | $35.6M | 7 |
| WHEATON FRANCISCAN HEALTHCARE - SOUTHEAST WISCONSIN INC | St Louis | E21 Health Care | $35.6M | 9 |
| AMEREN MANAGEMENT HEALTH TRUST | Saint Louis | — | $35.5M | 1 |
| CARESTL HEALTH | St Louis | — | $35.5M | 17 |
| GUADALUPE EDUCATIONAL SYSTEM INC | Kansas City | B29 Education | $35.3M | 3 |
| PATHWAYS PSYCHIATRIC HOSPITAL INC | Windsor | F31 Mental Health & Crisis Intervention | $35.1M | 13 |
| MISSOURI HISTORICAL SOCIETY | St Louis | A540 Arts, Culture & Humanities | $35.1M | 8 |
| OZARK TRI-COUNTY HEALTH CARE CONSORTIUM | Neosho | L80Z Housing & Shelter | $34.7M | 12 |
| WEST COMMUNITY CREDIT UNION | Ofallon | — | $34.6M | 12 |
| FRONTIER SCHOOLS INC | Kansas City | B29 Education | $34.6M | 2 |
| OZARK BORDER ELECTRIC COOPERATIVE INC | Poplar Bluff | — | $34.4M | 21 |
| JACKSON COUNTY COMMUNITY CHILDREN'S SERVICES FUND | Kansas City | P30 Human Services | $34.4M | 3 |
| AMERICAN PUBLIC WORKS ASSOCIATION | Kansas City | W00C Public & Societal Benefit | $34.4M | 7 |
| WILLIAM T KEMPER FOUNDATION COMMERCE BANK TRUSTEE | Kansas City | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $34.3M | 2 |
