| Sweetwater Hospital Association | Sweetwater | E22Z Health Care | $75.4M | 19 |
| LEADERS CREDIT UNION | Jackson | W61 Public & Societal Benefit | $75.4M | 8 |
| Wellmont Cardiology Services | Johnson City | E30 Health Care | $74.4M | 25 |
| POWELL VALLEY ELECTRIC COOPERATIVE | New Tazewell | — | $74.4M | 16 |
| SHEET METAL WORKERS NATIONAL HEALTH FUND | Goodletsville | — | $72.5M | 1 |
| Ballad Health | Johnson City | E19 Health Care | $72.1M | 14 |
| OPERATION COMPASSION | Cleveland | X20 Religion-Related | $72M | 0 |
| MID-SOUTH FOOD BANK | Memphis | K31Z Food, Agriculture & Nutrition | $71.8M | 4 |
| MOUNTAIN ELECTRIC COOPERATIVE INC | Mountain City | — | $71.6M | 10 |
| ALLIANCE HEALTHCARE SERVICES | Memphis | — | $71.6M | 9 |
| TENNESSEE LIFE & HEALTH INSURANCE | Nashville | — | $71.1M | 0 |
| THE ENSWORTH SCHOOL | Nashville | B240 Education | $71M | 7 |
| KIPP NASHVILLE | Whites Creek | B29 Education | $70.8M | 10 |
| TWIN LAKES TELEPHONE COOPERATIVE CORP | Gainesboro | — | $70.5M | 18 |
| FREED-HARDEMAN UNIVERSITY | Henderson | B420 Education | $69.2M | 10 |
| UT LE BONHEUR PEDIATRIC SPECIALISTS INC | Memphis | E300 Health Care | $69.1M | 18 |
| CHRISTIAN BROTHERS UNIVERSITY | Memphis | B430 Education | $68.8M | 7 |
| FIRST HORIZON CORPORATION EMPLOYEES' | Memphis | — | $68.4M | 0 |
| COUNTRY MUSIC ASSOCIATION INC | Nashville | A68Z Arts, Culture & Humanities | $68.4M | 14 |
| CREWS FAMILY FOUNDATION | Memphis | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $68M | 0 |
| MEDICAL EDUCATION ASSISTANCE CORP | Mountain Home | — | $68M | 30 |
| SOUTHEAST COMMUNITY CAPITAL CORPORATION | Nashville | J20 Employment | $65.1M | 14 |
| THE TROUSDALE FOUNDATION INC | Cleveland | — | $65.1M | 2 |
| MARYVILLE COLLEGE | Maryville | B420 Education | $64.3M | 11 |
| THE MCCALLIE SCHOOL | Chattanooga | B200 Education | $64.2M | 11 |
| CHRISTIAN ADVOCATES SERVING | Brentwood | R600 Civil Rights, Social Action & Advocacy | $62.7M | 11 |
| OPEN ARMS CARE CORPORATION | Brentwood | P730 Human Services | $62.5M | 6 |
| YMCA OF MEMPHIS & THE MID-SOUTH | Cordova | P27Z Human Services | $62.2M | 6 |
| First Horizon Foundation | Memphis | — | $62.2M | 1 |
| CHRIST COMMUNITY HEALTH SERVICES INC | Memphis | E39Z Health Care | $61.6M | 11 |
| THE STERN CARDIOVASCULAR FOUNDATION | Germantown | E32 Health Care | $61.3M | 20 |
| UNITED FURNITURE WORKERS INSURANCE FUND | Nashville | — | $60.2M | 1 |
| MUSIC CITY PREP CLINIC | Nashville | E32 Health Care | $59.5M | 2 |
| DUCKS UNLIMITED INC GROUP RETURN | Memphis | C300 Environment | $59.3M | 14 |
| CARSON-NEWMAN UNIVERSITY | Jefferson City | B420 Education | $58.7M | 13 |
| MILLIGAN UNIVERSITY | Milligan | B42Z Education | $58.3M | 11 |
| Vine International Inc | Knoxville | E60Z Health Care | $58.2M | 3 |
| COUNTRY MUSIC FOUNDATION INC | Nashville | A500 Arts, Culture & Humanities | $57.1M | 9 |
| FISK UNIVERSITY | Nashville | B43 Education | $56.2M | 15 |
| UNIVERSITY OF CHATTANOOGA | Chattanooga | B11 Education | $56M | 0 |
| BAPTIST MEMORIAL HOSPITAL-UNION CITY | Union City | E220 Health Care | $54.8M | 13 |
| SISKIN HOSPITAL FOR PHYSICAL | Chattanooga | E220 Health Care | $54.8M | 13 |
| SOUTHERN OPERATORS HEALTH FUND | Goodlettsville | Y43 Mutual & Membership Benefit | $54.6M | 0 |
| CUMBERLAND HEIGHTS FOUNDATION INC | Nashville | F220 Mental Health & Crisis Intervention | $52.5M | 10 |
| UNITY MEDICAL CENTER INC | Manchester | E22 Health Care | $51.7M | 13 |
| BAYLOR SCHOOL | Chattanooga | B250 Education | $51.5M | 12 |
| PLATEAU ELECTRIC COOPERATIVE | Oneida | — | $51.3M | 19 |
| MEMPHIS GOODWILL INC | Memphis | P29 Human Services | $51.1M | 2 |
| PICKWICK ELECTRIC COOPERATIVE | Selmer | — | $50.3M | 17 |
| Fully Alive Foundation Inc | Knoxville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $50M | 0 |
