| DEPAUL COMMUNITY RESOURCES | Roanoke | — | $24.3M | 2 |
| THE LANGLEY SCHOOL INC | McLean | B21Z Education | $24.2M | 7 |
| BEACON CREDIT UNION INC | Lynchburg | — | $24.2M | 15 |
| AMERICAN EDGE | Vienna | — | $24.1M | 4 |
| National Association of State Mental | Alexandria | F32Z Mental Health & Crisis Intervention | $24M | 8 |
| WASHINGTON GOLF & COUNTRY CLUB INC | Arlington | — | $24M | 10 |
| AMERICAN ASSOCIATION OF AIRPORT | Alexandria | — | $24M | 12 |
| Carilion Tazewell Community Hospital | Roanoke | E22Z Health Care | $23.8M | 15 |
| THE GENAN FOUNDATION | Charlottesville | — | $23.7M | 4 |
| SKILLSUSA INC | Leesburg | J200 Employment | $23.7M | 4 |
| CHOICE BOOKS OF NORTHERN VIRGINIA INC | Bristow | X99Z Religion-Related | $23.6M | 3 |
| ALL RISE FOR JUSTICE | Alexandria | F220 Mental Health & Crisis Intervention | $23.4M | 13 |
| SOAR 365 | Richmond | — | $23.4M | 13 |
| FEDERATION OF STATE BOARDS OF PHYSICAL | Alexandria | — | $23.4M | 19 |
| ST CHARLES HEALTH COUNCIL INC | Jonesville | — | $23.3M | 9 |
| ASIS INTERNATIONAL INC | Alexandria | — | $23.1M | 10 |
| LEXINGTON RETIREMENT COMMUNITY INC | Lexington | P750 Human Services | $23.1M | 5 |
| RAPPAHANNOCK WESTMINSTER-CANTERBURY INC | Irvington | P750 Human Services | $23M | 8 |
| AMERICAN SOCIETY FOR RADIATION ONCOLOGY | Arlington | — | $22.7M | 18 |
| ASSOCIATION OF SOCIAL WORK BOARDS | Culpeper | W70 Public & Societal Benefit | $22.6M | 7 |
| COMMUNITY ACCESS NETWORK INC | Lynchburg | E21 Health Care | $22.5M | 7 |
| MILITARY OFFICERS ASSOCIATION OF AMERICA | Alexandria | W30 Public & Societal Benefit | $22.5M | 9 |
| Global Impact Social Welfare Fund | Alexandria | W01 Public & Societal Benefit | $22.5M | 0 |
| COMMONWEALTH CATHOLIC CHARITIES | Richmond | P20Z Human Services | $22.4M | 3 |
| MARINE CORPS SCHOLARSHIP FOUNDATION INC | Alexandria | B82Z Education | $22.4M | 8 |
| CASUALTY ACTUARIAL SOCIETY | Arlington | — | $22.3M | 10 |
| Reston Association | Reston | — | $22.2M | 8 |
| BBB NATIONAL PROGRAMS INC | McLean | — | $22.2M | 8 |
| CORE KNOWLEDGE FOUNDATION | Charlottesville | B190 Education | $22.1M | 6 |
| DENTISTRY VCU | Richmond | — | $22.1M | 13 |
| Protect Women Ohio Action Inc | Arlington | — | $22.1M | 2 |
| ASSOCIATION FOR PROFESSIONAL IN | Arlington | E72C Health Care | $21.9M | 10 |
| THE CHRYSLER MUSEUM INCORPORATED | Norfolk | A510 Arts, Culture & Humanities | $21.9M | 4 |
| SOUTHWEST VIRGINIA COMMUNITY HEALTH | Meadowview | E32 Health Care | $21.9M | 6 |
| VIRGINIA MENNONITE HOME INC | Harrisonburg | E910 Health Care | $21.9M | 7 |
| CAPITAL ONE FOUNDATION INC | McLean | B82Z Education | $21.8M | 0 |
| VIRGINIA NONPROFIT HOUSING COALITION | Richmond | — | $21.7M | 0 |
| FREDERICKSBURG REGIONAL FOOD BANK | Fredericksburg | K310 Food, Agriculture & Nutrition | $21.7M | 2 |
| CHC CREATING HEALTHIER COMMUNITIES | Alexandria | — | $21.7M | 10 |
| SOKOL FAMILY FOUNDATION | Virginia Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $21.7M | 1 |
| JAMES MADISON UNIVERSITY FOUNDATION INC | Harrisonburg | B820 Education | $21.6M | 3 |
| WEISSBERG FOUNDATION | McLean | — | $21.5M | 5 |
| THE GLEBE INC | Glen Allen | L22 Housing & Shelter | $21.5M | 11 |
| MOUNTAIN EMPIRE OLDER CITIZENS INC | Big Stone Gap | P810 Human Services | $21.5M | 2 |
| World Hope International Inc | Alexandria | Q30 International, Foreign Affairs & National Security | $21.4M | 9 |
| UNITED WAY OF THE NATIONAL CAPITAL AREA | Vienna | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $21.2M | 9 |
| THE SOCIETY OF ST ANDREW INC | Big Island | K300 Food, Agriculture & Nutrition | $21.2M | 1 |
| Congressional School Inc | Falls Church | — | $21.1M | 7 |
| VCU Health Childrens Services at Brook Road | Richmond | E24 Health Care | $21.1M | 13 |
| THE COMMUNITY FOUNDATION FOR NORTHERN | Fairfax | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $21M | 3 |
