| ASSOCIATED GENERAL CONTRACTORS OF AMERICA INC | Arlington | — | $24.7M | 9 |
| VIRGINIA HISTORICAL SOCIETY | Richmond | A800 Arts, Culture & Humanities | $24.6M | 6 |
| EPWORTH SENIOR COMMUNITY FOUNDATION C/O STERLING FOUNDATION MANAGEMENT LLC | Reston | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $24.6M | 0 |
| National Society of Black Engineers | Alexandria | U40C Science & Technology | $24.6M | 10 |
| THE STEWARD SCHOOL | Richmond | — | $24.4M | 6 |
| THE HARVEST FOUNDATION OF THE PIEDMONT | Martinsville | X00Z Religion-Related | $24.3M | 1 |
| AMERICAN ASSOCIATION FOR THE STUDY OF LIVER DISEASES FOUNDATION | Alexandria | E11 Health Care | $24.3M | 5 |
| ARLINGTON PARTNERSHIP FOR AFFORDABLE HOUSING | Arlington | — | $24.3M | 9 |
| Atlas Economic Research Foundation | Arlington | V22Z Social Science | $24.3M | 10 |
| DEPAUL COMMUNITY RESOURCES | Roanoke | — | $24.3M | 2 |
| VIRGINIA MENNONITE HOME INC | Harrisonburg | E910 Health Care | $24.2M | 7 |
| BEACON CREDIT UNION INC | Lynchburg | — | $24.2M | 15 |
| AMERICAN EDGE | Vienna | — | $24.1M | 4 |
| HOPELINK BEHAVIORAL HEALTH | Oakton | — | $24.1M | 8 |
| National Association of State Mental Health Program Directors | Alexandria | F32Z Mental Health & Crisis Intervention | $24M | 8 |
| WASHINGTON GOLF & COUNTRY CLUB INC | Arlington | — | $24M | 10 |
| PATHWAY HOMES INC | Fairfax | F33Z Mental Health & Crisis Intervention | $23.9M | 7 |
| Carilion Tazewell Community Hospital | Roanoke | E22Z Health Care | $23.8M | 15 |
| ASSOCIATION OF SOCIAL WORK BOARDS | Culpeper | W70 Public & Societal Benefit | $23.8M | 7 |
| THE GENAN FOUNDATION | Charlottesville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $23.7M | 4 |
| SKILLSUSA INC | Leesburg | J200 Employment | $23.7M | 4 |
| ROBINS FOUNDATION | Richmond | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $23.7M | 1 |
| BLUE INVESTMENTS FOR NATURE INC | Arlington | C30 Environment | $23.7M | 5 |
| ST JOSEPH'S VILLA | Richmond | P700 Human Services | $23.6M | 8 |
| CHOICE BOOKS OF NORTHERN VIRGINIA INC | Bristow | X99Z Religion-Related | $23.6M | 3 |
| ALL RISE FOR JUSTICE | Alexandria | F220 Mental Health & Crisis Intervention | $23.4M | 13 |
| ST CHARLES HEALTH COUNCIL INC | Jonesville | — | $23.3M | 9 |
| THE CLAUDE MOORE CHARITABLE FOUNDATION | Fairfax | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $23.1M | 7 |
| LEXINGTON RETIREMENT COMMUNITY INC | Lexington | P750 Human Services | $23.1M | 5 |
| RAPPAHANNOCK WESTMINSTER-CANTERBURY INC | Irvington | P750 Human Services | $23M | 8 |
| COPTIC ORPHANS SUPPORT ASSOCIATION | Merrifield | Q330 International, Foreign Affairs & National Security | $23M | 6 |
| VIRGINIA AFFILIATE OF THE HORSEMEN'S BENEVOLENT & PROTECTIVE ASSOCIATION INC | Warrenton | S41 Community Improvement & Capacity Building | $22.9M | 1 |
| COMMUNITY HEALTH ACCREDITATION PROGRAM INC | Arlington | E03 Health Care | $22.7M | 8 |
| NATIONAL CAPITAL TREATMENT AND RECOVERY | Arlington | F200 Mental Health & Crisis Intervention | $22.6M | 10 |
| COMMUNITY ACCESS NETWORK INC | Lynchburg | E21 Health Care | $22.5M | 7 |
| VPM MEDIA CORPORATION | Richmond | A300 Arts, Culture & Humanities | $22.5M | 10 |
| MILITARY OFFICERS ASSOCIATION OF AMERICA | Alexandria | W30 Public & Societal Benefit | $22.5M | 9 |
| BOULDER CREST FOUNDATION | Bluemont | P80 Human Services | $22.4M | 10 |
| CASUALTY ACTUARIAL SOCIETY | Arlington | — | $22.3M | 10 |
| PALESTINIAN AMERICAN MEDICAL ASSOCIATION | Fairfax | Q30 International, Foreign Affairs & National Security | $22.3M | 1 |
| Reston Association | Reston | — | $22.2M | 8 |
| BBB NATIONAL PROGRAMS INC | McLean | — | $22.2M | 8 |
| DENTISTRY VCU | Richmond | — | $22.1M | 13 |
| Protect Women Ohio Action Inc | Arlington | — | $22.1M | 2 |
| GREATER CHARLOTTESVILLE HABITAT FOR HUMANITY INC | Charlottesville | — | $21.9M | 5 |
| ASSOCIATION FOR PROFESSIONAL IN INFECTION CONTROL & EPIDEMOLOGY INC | Arlington | E72C Health Care | $21.9M | 10 |
| THE CHRYSLER MUSEUM INCORPORATED | Norfolk | A510 Arts, Culture & Humanities | $21.9M | 4 |
| PIPELINE RESEARCH COUNCIL INTERNATIONAL INC | Chantilly | S03 Community Improvement & Capacity Building | $21.9M | 11 |
| SOUTHWEST VIRGINIA COMMUNITY HEALTH SYSTEMS INC | Meadowview | E32 Health Care | $21.9M | 6 |
| VIRGINIA INSTITUTE OF AUTISM INC | Charlottesville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $21.9M | 1 |
