| BELLE HAVEN COUNTRY CLUB INC | Alexandria | — | $28.2M | 7 |
| NATL ASSN OF SECONDARY SCHOOL PRINCIPALS | Reston | B25C Education | $28.1M | 11 |
| American Bird Conservancy | Marshall | D013 Animal-Related | $27.9M | 9 |
| OAK SPRING GARDEN FOUNDATION | Upperville | X83 Religion-Related | $27.8M | 2 |
| FRIENDSHIP FOUNDATION | Roanoke | T22Z Philanthropy, Voluntarism & Grantmaking Foundations | $27.8M | 8 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION | Newport News | P27Z Human Services | $27.7M | 7 |
| CAPE HENRY COLLEGIATE SCHOOL INC | Virginia Beach | B200 Education | $27.2M | 7 |
| THE VOLGENAU FOUNDATION | McLean | C34Z Environment | $27.2M | 6 |
| WELLSPRING FOUNDATION OF SOUTHWEST | Abingdon | E112 Health Care | $27.1M | 4 |
| AMERICAN INSTITUTE OF AERONAUTICS AND | Reston | — | $27.1M | 14 |
| Roman Eagle Rehabilitation and | Danville | — | $27M | 6 |
| APPALACHIAN AGENCY FOR SR CITIZENS | Cedar Bluff | P810 Human Services | $27M | 6 |
| ARMY EMERGENCY RELIEF | Arlington | W300 Public & Societal Benefit | $26.9M | 9 |
| THE JAMES FAMILY CHARITABLE | Charlottesville | T30J Philanthropy, Voluntarism & Grantmaking Foundations | $26.8M | 0 |
| AEROSPACE INDUSTRIES ASSOCIATION | Arlington | — | $26.8M | 11 |
| NATIONAL AUTOMATED CLEARING HOUSE | Reston | — | $26.8M | 10 |
| WATER ENVIRONMENT FEDERATION | Alexandria | C022 Environment | $26.8M | 11 |
| ENGINEERING CONSULTING SERVICES LTD | Chantilly | — | $26.5M | 2 |
| HOSPICE OF THE PIEDMONT INC | Charlottesville | — | $26.4M | 11 |
| RANDOLPH COLLEGE INC | Lynchburg | B420 Education | $26.4M | 9 |
| VMI FOUNDATION | Lexington | B110 Education | $26.3M | 7 |
| National Electrical Manufacturers Association | Arlington | — | $26.2M | 12 |
| MUSLIM AID AMERICA | McLean | M20 Public Safety, Disaster Preparedness & Relief | $26.2M | 1 |
| AFCEA INTERNATIONAL | Fairfax | — | $26.1M | 8 |
| BLUEFIELD UNIVERSITY | Bluefield | B420 Education | $25.9M | 6 |
| Spirit of America Worldwide | Arlington | Q30 International, Foreign Affairs & National Security | $25.9M | 12 |
| ELDERHOMES CORPORATION | Richmond | L810 Housing & Shelter | $25.9M | 6 |
| PHILLIPS PROGRAMS | Annandale | B28Z Education | $25.8M | 8 |
| MEALS ON WHEELS AMERICA | Arlington | K30C Food, Agriculture & Nutrition | $25.5M | 13 |
| STATE POLICY NETWORK | Arlington | R02 Civil Rights, Social Action & Advocacy | $25.2M | 10 |
| SHERWOOD FUND | Middleburg | T23 Philanthropy, Voluntarism & Grantmaking Foundations | $25.2M | 0 |
| AMERICAN INTERNATIONAL SCHOOL OF LAGOS | Alexandria | B20 Education | $25.1M | 0 |
| NATIONAL CAPITAL TREATMENT AND RECOVERY | Arlington | F200 Mental Health & Crisis Intervention | $25M | 10 |
| CHATHAM HALL | Chatham | B250 Education | $25M | 5 |
| VIRGINIA BAPTIST CHILDREN'S HOME | Salem | — | $25M | 2 |
| NATIONAL BOARD FOR PROFESSIONAL TEACHING | Arlington | B030 Education | $24.9M | 7 |
| Susan B Anthony List Inc | Arlington | B99 Education | $24.9M | 10 |
| VIRGINIA UNITED METHODIST HOMES | Glen Allen | P75 Human Services | $24.9M | 7 |
| NATIONAL ASSOCIATION OF FEDERALLY- | Arlington | — | $24.8M | 8 |
| VPM MEDIA CORPORATION | Richmond | A300 Arts, Culture & Humanities | $24.8M | 9 |
| PRESBYTERIAN HOMES & FAMILY SVCS | Lynchburg | P700 Human Services | $24.8M | 5 |
| NATIONAL RECREATION AND PARK ASSOCIATION | Ashburn | C300 Environment | $24.7M | 10 |
| ASSOCIATED GENERAL CONTRACTORS | Arlington | — | $24.7M | 9 |
| National Society of Black Engineers | Alexandria | U40C Science & Technology | $24.6M | 10 |
| THE STEWARD SCHOOL | Richmond | — | $24.4M | 6 |
| VIRGINIA AFFILIATE OF THE HORSEMEN'S | Warrenton | S41 Community Improvement & Capacity Building | $24.4M | 1 |
| THE HARVEST FOUNDATION OF THE PIEDMONT | Martinsville | X00Z Religion-Related | $24.3M | 1 |
| AMERICAN ASSOCIATION FOR THE STUDY OF | Alexandria | E11 Health Care | $24.3M | 5 |
| ARLINGTON PARTNERSHIP FOR AFFORDABLE | Arlington | — | $24.3M | 9 |
| Railroad Employees National Health | Arlington | Y43 Mutual & Membership Benefit | $24.3M | 1 |
