| HEALTHNET AEROMEDICAL SERVICES INC | Charleston | E62Z Health Care | $48M | 13 |
| BETHANY COLLEGE | Bethany | B420 Education | $45.6M | 5 |
| MOUNTAINEER FOOD BANK INC | Gassaway | K31Z Food, Agriculture & Nutrition | $45.3M | 3 |
| Charleston Hospital Inc | Charleston | — | $43.7M | 10 |
| West Virginia University Medical Corp Charleston Division DBA WVUPC | Charleston | B99 Education | $42M | 13 |
| WVUHS Home Care LLC | Morgantown | — | $40.4M | 11 |
| International Union of Operating Engineers Local 132 Health & Welfare Fd | Huntington | — | $39.9M | 0 |
| Wetzel County Hospital Inc | New Martinsville | E22 Health Care | $39.2M | 9 |
| DAVIS & ELKINS COLLEGE | Elkins | B420 Education | $37.9M | 7 |
| WEST VIRGINIA HEALTH RIGHT INC | Charleston | — | $37.8M | 6 |
| Northwood Health System | Wheeling | F320 Mental Health & Crisis Intervention | $37.4M | 3 |
| MARYLAND ELECTRICAL INDUSTRY HEALTH FUND | Ona | — | $36.9M | 0 |
| LINCOLN COUNTY PRIMARY CARE CENTER INC | Hamlin | E320 Health Care | $36.6M | 7 |
| GOODWILL INDUSTRIES OF KANAWHA VALLEY INC | Charleston | — | $36.5M | 4 |
| WEST VIRGINIA LABORERS' TRUST FUND | Charleston | — | $36.5M | 1 |
| SHENANDOAH VALLEY MEDICAL SYSTEM I | Martinsburg | E39Z Health Care | $36.5M | 8 |
| WOMENCARE INC | Scott Depot | E32Z Health Care | $36.3M | 8 |
| MONTGOMERY GENERAL HOSPITAL INC | Montgomery | E22Z Health Care | $35.1M | 14 |
| WESTBROOK HEALTH SERVICES INC | Parkersburg | F20Z Mental Health & Crisis Intervention | $35M | 8 |
| RIVER VALLEY CHILD DEVELOPMENT SERVICES INC | Huntington | P33Z Human Services | $34.7M | 3 |
| E A HAWSE HEALTH CENTER | Baker | E300 Health Care | $33.8M | 7 |
| New River Health Association Inc | Oak Hill | E320 Health Care | $33.6M | 9 |
| RAINELLE MEDICAL CENTER INC | Rainelle | E32Z Health Care | $32.2M | 7 |
| Braxton County Memorial Hospital Inc | Gassaway | E22Z Health Care | $31.7M | 14 |
| THE CHILDREN'S HOME SOCIETY OF WEST VIRGINIA INC | Charleston | P30Z Human Services | $31.7M | 2 |
| MINNIE HAMILTON HEALTH CARE CENTER INC | Grantsville | E22 Health Care | $31.3M | 7 |
| WELTY HOME FOR THE AGED INC | Wheeling | P75I Human Services | $31.2M | 7 |
| QUALITY INSIGHTS INC | Charleston | — | $29.4M | 19 |
| MARIETTA MEMORIAL HOSPITAL OF TYLER COUNTY INC | Sistersville | E20 Health Care | $29.1M | 10 |
| CORTLAND ACRES ASSOCIATION INC | Thomas | P750 Human Services | $28M | 3 |
| Employer-Teamsters Local Nos 175 & 505 Health & Welfare Fund | South Charleston | — | $27.7M | 3 |
| CABIN CREEK HEALTH CENTER INC | Dawes | X00Z Religion-Related | $27.4M | 8 |
| UNITED FOOD & COMMERICIAL WORKERS LOCAL 400 AND EMPLOYERS HEALTH & WELFARE FUND | South Charleston | — | $27.1M | 0 |
| HIGHLAND-CLARKSBURG HOSPITAL INC | Clarksburg | E22 Health Care | $27M | 11 |
| KANAWHA HOSPICE CARE INC | Charleston | P74Z Human Services | $26.8M | 7 |
| SOUTHERN HIGHLANDS COMMUNITY MENTAL HEALTH CENTER INC | Princeton | — | $26.2M | 7 |
| Wheeling Jesuit University Inc | Wheeling | B430 Education | $25.6M | 6 |
| MYLAN PARK FOUNDATION INC | Morgantown | B112 Education | $25.5M | 2 |
| TRI-COUNTY BUILDING TRADES HEALTH FUND | Ona | — | $25.4M | 0 |
| SOUTHWEST LMC | Ona | N62 Recreation & Sports | $25.2M | 0 |
| COALFIELD DEVELOPMENT CORPORATION | Wayne | L99 Housing & Shelter | $23.4M | 5 |
| CHANGE INC | Weirton | J20Z Employment | $23.3M | 9 |
| WIRT COUNTY HEALTH SERVICES ASSOCIATION INC | Elizabeth | E39Z Health Care | $23.1M | 6 |
| THE GREATER KANAWHA VALLEY FOUNDATION | Charleston | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $23.1M | 2 |
| VALLEY HEALTH CARE INC | Mill Creek | Z99Z Unknown | $22.6M | 8 |
| FACING HUNGER FOODBANK INC | Huntington | — | $22.3M | 0 |
| MARSHALL UNIVERSITY FOUNDATION INCORPORATED | Huntington | B110 Education | $22.2M | 3 |
| BROADDUS HOSPITAL ASSOCIATION INC | Philippi | E220 Health Care | $22.2M | 8 |
| WEST VIRGINIA STATE UNIVERSITY RESEARCH & DEVELOPMENT CORPORATION | Institute | — | $21.9M | 2 |
| PARTNER COMMUNITY CAPITAL INC | Charles Town | P20 Human Services | $21.7M | 8 |
