| Gundersen Clinic Ltd | La Crosse | E220 Health Care | $244.3M | 42 |
| ThedaCare Regional Medical Center - Appleton Inc | Appleton | E220 Health Care | $237.8M | 0 |
| THE MONROE CLINIC INC | Monroe | E220 Health Care | $237.1M | 14 |
| COVANTAGE CREDIT UNION | Antigo | — | $234.4M | 21 |
| EDUCATORS CREDIT UNION | Mt Pleasant | — | $231.7M | 29 |
| GROUP HEALTH COOPERATIVE OF EAU CLAIRE | Eau Claire | E81Z Health Care | $230.1M | 4 |
| Thedacare Inc | Appleton | E20 Health Care | $210.8M | 0 |
| PROHEALTH MEDICAL GROUP INC | Waukesha | E20 Health Care | $204.3M | 8 |
| GOODWILL RETAIL SERVICES INC | Milwaukee | J32 Employment | $203.5M | 9 |
| HERZING UNIVERSITY LTD | Milwaukee | B40 Education | $200.5M | 23 |
| MILWAUKEE SCHOOL OF ENGINEERING | Milwaukee | B500 Education | $192.1M | 14 |
| AURORA HEALTH CARE INC | Milwaukee | E21 Health Care | $184.6M | 30 |
| PROHEALTH CARE INC | Waukesha | — | $181.9M | 16 |
| FOX COMMUNITIES CREDIT UNION | Appleton | — | $180.6M | 22 |
| ASPIRUS RIVERVIEW HOSPITAL & CLINICS INC | Wisconsin Rapids | E220 Health Care | $178.2M | 24 |
| OPERATING ENGINEERS LOCAL 139 HEALTH BENEFIT FUND | Pewaukee | — | $177.2M | 4 |
| CONCORDIA UNIVERSITY INC | Mequon | B420 Education | $174.2M | 9 |
| THE ROBERT D AND PATRICIA E KERN FAMILY FOUNDATION INC | Waukesha | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $170.2M | 9 |
| ASPIRUS STEVENS POINT HOSPITAL & CLINICS INC | Stevens Point | E220 Health Care | $166.4M | 24 |
| US BANK FOUNDATION | Milwaukee | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $165.6M | 1 |
| MEMORIAL MEDICAL CENTER INC | Ashland | E220 Health Care | $164.7M | 11 |
| CAPITAL CREDIT UNION | Green Bay | — | $162.8M | 17 |
| CATHOLIC FINANCIAL LIFE | Milwaukee | — | $161.8M | 23 |
| CNH WELFARE BENEFIT TRUST | Racine | Y43 Mutual & Membership Benefit | $161.5M | 1 |
| FORT HEALTHCARE INC | Fort Atkinson | E220 Health Care | $160.7M | 13 |
| LAWRENCE UNIVERSITY OF WISCONSIN | Appleton | B420 Education | $154.4M | 11 |
| LAKEVIEW MEDICAL CENTER INC OF RICE LAKE | Rice Lake | E220 Health Care | $152.6M | 7 |
| ThedaCare Regional Medical Center - Neenah Inc | Appleton | E220 Health Care | $150.9M | 0 |
| St Joseph's Community Hospital of West Bend Inc | West Bend | E220 Health Care | $149.2M | 6 |
| ST CROIX REGIONAL MEDICAL CENTER | St Croix Falls | E20 Health Care | $148.1M | 13 |
| St Mary's Hospital Medical Center of Green Bay Inc - Hospital Sisters | Green Bay | E220 Health Care | $141.4M | 10 |
| WISCONSIN ALUMNI RESEARCH FOUNDATION | Madison | B840 Education | $140.8M | 23 |
| CARTHAGE COLLEGE | Kenosha | B430 Education | $139.7M | 11 |
| PROHEALTH OCONOMOWOC MEMORIAL HOSPITAL INC | Oconomowoc | E220 Health Care | $138.7M | 8 |
| CARROLL UNIVERSITY INC | Waukesha | B420 Education | $137.5M | 15 |
| SAUK PRAIRIE HEALTHCARE INC | Prairie Du Sac | E220 Health Care | $136.8M | 20 |
| CHILDREN'S HOSPITAL OF WISCONSIN FOUNDATION INC | Milwaukee | E112 Health Care | $134.2M | 13 |
| Sacred Heart Hospital of Hospital Sisters of Third Order of St Francis | Eau Claire | E220 Health Care | $133.9M | 9 |
| LANGLADE HOSPITAL - HOTEL DIEU OF ST JOSEPH OF ANTIGO WISCONSIN | Antigo | E22Z Health Care | $128.6M | 24 |
| ASPIRUS MEDICAL GROUP INC | Wausau | E32Z Health Care | $128.4M | 23 |
| TELEPHONE AND DATA SYSTEMS INC EMPLOYEE BENEFIT TRUST | Madison | — | $125.5M | 0 |
| COMMUNITY CARE HEALTH PLAN INC | Brookfield | E31 Health Care | $125.5M | 7 |
| ST NORBERT COLLEGE INC | De Pere | B420 Education | $124.8M | 14 |
| WISCONSIN LABORERS' HEALTH FUND | Deforest | — | $124.2M | 9 |
| DOOR COUNTY MEMORIAL HOSPITAL | Sturgeon Bay | E220 Health Care | $120.1M | 11 |
| MILE BLUFF MEDICAL CENTER | Mauston | E220 Health Care | $119.4M | 12 |
| SOUTHWEST HEALTH CENTER INC | Platteville | E22Z Health Care | $119M | 17 |
| St Nicholas Hospital of the Hospital Sisters of the Third Order of St Francis | Sheboygan | E220 Health Care | $118.1M | 10 |
| WESTCONSIN CREDIT UNION | Menomonie | — | $115.6M | 21 |
| CHILDREN'S MEDICAL GROUP INC | Milwaukee | E20Z Health Care | $113.5M | 26 |
