| AURORA MEDICAL CENTER BAY AREA INC | Milwaukee | E220 Health Care | $113.3M | 26 |
| ST MARY'S HOSPITAL OF SUPERIOR | Superior | — | $113.2M | 17 |
| UPLAND HILLS HEALTH INC | Dodgeville | E220 Health Care | $112.5M | 8 |
| REEDSBURG AREA MEDICAL CENTER | Reedsburg | E220 Health Care | $111.6M | 11 |
| GOODWILL INDUSTRIES OF SOUTHEASTERN WISCONSIN INC | Milwaukee | J320 Employment | $110.7M | 8 |
| INTERNATIONAL CHILDREN'S FUND INC | Neenah | — | $109.1M | 2 |
| Agrace HospiceCare Inc | Fitchburg | — | $107.5M | 12 |
| GOODWILL INDUSTRIES OF NORTH CENTRAL WISCONSIN INC | Menasha | P20Z Human Services | $106.7M | 8 |
| STATEWIDE ENERGY EFFICIENCY AND RENEWABLES ADMINISTRATION INC | LaCrosse | S41 Community Improvement & Capacity Building | $104.8M | 0 |
| WORKMONEY INC | Milwaukee | — | $103.1M | 12 |
| ASPIRUS MEDFORD HOSPITAL & CLINICS INC | Medford | E220 Health Care | $100.7M | 25 |
| BEAVER DAM COMMUNITY HOSPITALS INC | Beaver Dam | E220 Health Care | $99M | 4 |
| MEDICAL SERVICES INC | Hayward | E22 Health Care | $98.3M | 10 |
| UNITED MIGRANT OPPORTUNITY SERVICES UMOS INC | Milwaukee | P8XZ Human Services | $97.5M | 14 |
| NORTH CENTRAL STATES REGIONAL COUNCIL OF CARPENTERS' HEALTH FUND | Eau Claire | — | $96.7M | 11 |
| ABLELIGHT INC | Watertown | P70 Human Services | $96.3M | 8 |
| VERNON MEMORIAL HEALTHCARE INC | Viroqua | E22 Health Care | $95M | 9 |
| Ascendium Education Group Inc | Madison | B40 Education | $94.9M | 25 |
| OCONTO HOSPITAL AND MEDICAL CENTER | Oconto | E20 Health Care | $93.1M | 10 |
| COOPERATIVE EDUCATIONAL SERVICE AGENCY 6 | Oshkosh | B19 Education | $93.1M | 12 |
| Tomah Memorial Hospital | Tomah | E20 Health Care | $92.9M | 7 |
| MARINE CREDIT UNION | La Crosse | — | $92.3M | 21 |
| FEEDING AMERICA EASTERN WISCONSIN INC | Milwaukee | K310 Food, Agriculture & Nutrition | $90.4M | 7 |
| WISCONSIN ELECTRICAL EMPLOYEES HEALTH AND WELFARE PLAN | Madison | — | $90.1M | 1 |
| HOLY FAMILY MEMORIAL INC | Manitowoc | E220 Health Care | $88.3M | 9 |
| PESI INC | Eau Claire | P74 Human Services | $87.6M | 15 |
| MEDICAL COLLEGE OF WISCONSIN AFFILIATED HOSPITALS INC | Milwaukee | — | $87.4M | 2 |
| PRAIRIE RIDGE HEALTH INC | Columbus | E020 Health Care | $86M | 12 |
| GREATER MILWAUKEE FOUNDATION INC | Milwaukee | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $85.2M | 12 |
| ADAMS-COLUMBIA ELECTRIC COOPERATIVE | Friendship | — | $85M | 16 |
| THE MORGRIDGE INSTITUTE FOUNDATION INC | Madison | H11 Medical Research | $84.9M | 3 |
| VIVENT HEALTH INC | Milwaukee | G81Z Diseases, Disorders & Medical Disciplines | $83.6M | 10 |
| DIVINE SAVIOR HEALTHCARE INC | Portage | E22 Health Care | $82.6M | 23 |
| BELOIT COLLEGE | Beloit | B420 Education | $82.4M | 14 |
| BALDWIN AREA MEDICAL CENTER INC | Baldwin | E220 Health Care | $81.3M | 8 |
| THE RICHLAND HOSPITAL INC | Richland Center | E220 Health Care | $81.1M | 11 |
| BRADLEY IMPACT FUND INC | Milwaukee | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $79.5M | 4 |
| WISCONSIN HOUSING PRESERVATION CORP | Madison | L21 Housing & Shelter | $79.2M | 18 |
| HUDSON HOSPITAL INC | Hudson | E22Z Health Care | $77.6M | 15 |
| MILWAUKEE DRIVERS HEALTH AND WELFARE TRUST FUND | Milwaukee | — | $76.4M | 3 |
| Stoughton Hospital Association | Stoughton | E220 Health Care | $74.7M | 12 |
| WORKMONEY FOUNDATION INC | Milwaukee | P51 Human Services | $73.5M | 2 |
| WISCONSIN WORKERS COMPENSATION INSURANCE | Waukesha | — | $72.6M | 0 |
| VERVE CREDIT UNION | Oshkosh | — | $70.1M | 26 |
| BLACK RIVER HEALTH INC | Black River Falls | E22Z Health Care | $70M | 9 |
| MILWAUKEE WORLD FESTIVAL INC | Milwaukee | A680 Arts, Culture & Humanities | $70M | 12 |
| GIVE BACK FOUNDATION | Madison | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $69.7M | 1 |
| Prairie du Chien Memorial Hospital Association Inc | Prairie Du Chien | E22Z Health Care | $69M | 8 |
| WAUPUN MEMORIAL HOSPITAL | Waupun | E220 Health Care | $68.7M | 9 |
| LUTHERAN SOCIAL SERVICES OF WISCONSIN AND UPPER MICHIGAN INC | West Allis | P200 Human Services | $68.3M | 13 |
