| ENDOCRINE SOCIETY | Washington | G900 Diseases, Disorders & Medical Disciplines | $27.3M | 20 |
| THIRD WAY | Washington | R99 Civil Rights, Social Action & Advocacy | $27.2M | 10 |
| NATIONAL ASSOCIATION OF STUDENT PERSONNEL ADMINISTRATORS INC | Washington | B030 Education | $27.1M | 11 |
| AMERICAN FOREST & PAPER ASSOCIATION INC | Washington | — | $27M | 11 |
| American Society for Engineering Education | Washington | — | $27M | 5 |
| UNITED STATES LETTER CARRIERS MUTUAL BENEFIT ASSOCIATION | Washington | — | $27M | 7 |
| COUNCIL FOR PROFESSIONAL RECOGNITION INC | Washington | B600 Education | $26.9M | 16 |
| National Association of Counties | Washington | — | $26.8M | 11 |
| CENTER FOR NONPROFIT ADVANCEMENT BENEFITS TRUST | Washington | — | $26.6M | 4 |
| PAUL PUBLIC CHARTER SCHOOL INC | Washington | B25 Education | $26.6M | 7 |
| THE LAB SCHOOL OF WASHINGTON | Washington | — | $26.5M | 7 |
| INGENUITY PREP | Washington | B29 Education | $26.5M | 7 |
| FORD'S THEATRE SOCIETY | Washington | A650 Arts, Culture & Humanities | $26.4M | 8 |
| GREENPEACE FUND INC | Washington | C123 Environment | $26.3M | 8 |
| American Forests | Washington | C360 Environment | $26.3M | 11 |
| AMERICAN INVESTMENT COUNCIL | Washington | J03 Employment | $26.3M | 8 |
| AMERICAN PUBLIC HEALTH ASSOCIATION | Washington | E70Z Health Care | $25.9M | 11 |
| CHILDREN'S SCHOOL SERVICES | Washington | E11 Health Care | $25.9M | 11 |
| INTERNATIONAL FRANCHISE ASSOCIATION | Washington | — | $25.8M | 10 |
| STACY MARK REED FOUNDATION INC | Washington | E11 Health Care | $25.7M | 0 |
| TEACHING LAB | Washington | B19 Education | $25.6M | 9 |
| AMERICAN ASSOCIATION OF COMMUNITY COLLEGES | Washington | B034 Education | $25.6M | 16 |
| NATIONAL ASSOCIATION OF INDEPENDENT SCHOOLS INC | Washington | — | $25.6M | 12 |
| NATIONAL LEAGUE OF CITIES | Washington | — | $25.5M | 7 |
| FORMER PLAYER BENEFITS TRUST | Washington | Y43 Mutual & Membership Benefit | $25.5M | 6 |
| DEFENDING DEMOCRACY TOGETHER INSTITUTE | Washington | B90 Education | $25.3M | 5 |
| ISRAEL ON CAMPUS COALITION | Washington | B80 Education | $25.2M | 10 |
| COMMON CAUSE EDUCATION FUND | Washington | W20 Public & Societal Benefit | $25.1M | 8 |
| NATIONAL ASSOCIATION OF CONSERVATION DISTRICTS | Washington | C030 Environment | $25.1M | 6 |
| NATIONAL ALLIANCE OF STATE AND TERRITORIAL AIDS DIRECTORSDBA NASTAD | Washington | E30Z Health Care | $25.1M | 8 |
| EXECUTIVE LEADERSHIP COUNCIL | Washington | Z99 Unknown | $24.9M | 18 |
| Association of Corporate Counsel | Washington | — | $24.9M | 12 |
| ASSOCIATION FOR DIAGNOSTICS & LABORATORY MEDICINE | Washington | B99Z Education | $24.8M | 12 |
| Stoddard Baptist Global Care | Washington | L22 Housing & Shelter | $24.7M | 8 |
| No Labels | Washington | — | $24.6M | 8 |
| FOCUS FOR DEMOCRACY FUND | Washington | R20 Civil Rights, Social Action & Advocacy | $24.6M | 0 |
| Family Research Council | Washington | P01 Human Services | $24.5M | 10 |
| UFCW HEALTH INSURANCE PLAN FOR RETIREES | Washington | Y43 Mutual & Membership Benefit | $24.5M | 7 |
| THE ARMY DISTAFF FOUNDATION INC | Washington | P750 Human Services | $24.4M | 11 |
| AMERICAN SOCIETY OF ASSOCIATION EXECUTIVES | Washington | — | $24.3M | 11 |
| COALITION FOR GREEN CAPITAL D/B/A AMERICAN GREEN BANK CONSORTIUM | Washington | C35 Environment | $24.3M | 12 |
| NATIONAL ASSOCIATION FOR THE EDUCATION OF YOUNG CHILDREN | Washington | B030 Education | $24.3M | 10 |
| Rape Abuse & Incest National Network (RAINN) | Washington | F40 Mental Health & Crisis Intervention | $24.2M | 8 |
| EQUAL JUSTICE WORKS | Washington | I110 Crime & Legal-Related | $24M | 6 |
| Friendship Place | Washington | L41 Housing & Shelter | $23.9M | 7 |
| National Main Street Center Inc | Washington | S20 Community Improvement & Capacity Building | $23.9M | 7 |
| LEADINGAGE INC | Washington | L030 Housing & Shelter | $23.9M | 16 |
| SMART GROUP VSTD PLAN AND TRUST | Washington | Y43 Mutual & Membership Benefit | $23.9M | 4 |
| WASHINGTON HUMANE SOCIETY | Washington | D200 Animal-Related | $23.8M | 6 |
| THE ALLIANCE FOR CLIMATE PROTECTION | Washington | C30 Environment | $23.8M | 11 |
