| CHILDREN'S SCHOOL SERVICES | Washington | E11 Health Care | $25.8M | 10 |
| INTERNATIONAL FRANCHISE ASSOCIATION | Washington | — | $25.8M | 10 |
| GENERATION YOU EMPLOYED INC | Washington | O50 Youth Development | $25.8M | 10 |
| SAINT COLETTA OF GREATER WASHINGTON INC | Washington | Z99 Unknown | $25.6M | 6 |
| NATIONAL LEAGUE OF CITIES | Washington | — | $25.5M | 7 |
| FORMER PLAYER BENEFITS TRUST | Washington | Y43 Mutual & Membership Benefit | $25.5M | 6 |
| Emerald Cities Collaborative Inc | Washington | W99 Public & Societal Benefit | $25.4M | 5 |
| CORPORATION OF THE WASHINGTON LATIN | Washington | B29 Education | $25.3M | 6 |
| DEFENDING DEMOCRACY TOGETHER INSTITUTE | Washington | B90 Education | $25.3M | 5 |
| The Army Distaff Foundation Inc | Washington | P750 Human Services | $25.2M | 7 |
| COOPERATIVE LEAGUE OF THE UNITED STATES | Washington | — | $25.2M | 10 |
| ISRAEL ON CAMPUS COALITION | Washington | B80 Education | $25.2M | 10 |
| INGENUITY PREP | Washington | B29 Education | $25.1M | 7 |
| AMERICAN HOTEL & LODGING ASSOCIATION | Washington | — | $25.1M | 7 |
| NATIONAL ASSOCIATION OF | Washington | C030 Environment | $25.1M | 6 |
| EXECUTIVE LEADERSHIP COUNCIL | Washington | Z99 Unknown | $24.9M | 18 |
| Association of Corporate Counsel | Washington | — | $24.9M | 12 |
| NATIONAL ASSOCIATION OF STUDENT | Washington | B030 Education | $24.8M | 11 |
| PARTNERSHIP FOR PUBLIC SERVICE INC | Washington | J05 Employment | $24.7M | 9 |
| Stoddard Baptist Global Care | Washington | L22 Housing & Shelter | $24.7M | 8 |
| No Labels | Washington | — | $24.6M | 8 |
| AMERICA'S AGENDA HEALTHCARE FOR ALL | Washington | E018 Health Care | $24.5M | 2 |
| WASHINGTON INSTITUTE FOR NEAR EAST | Washington | Q055 International, Foreign Affairs & National Security | $24.4M | 10 |
| AMERICAN INVESTMENT COUNCIL | Washington | J03 Employment | $24.4M | 8 |
| JUBILEE HOUSING INC | Washington | — | $24.4M | 5 |
| REBUILDING TOGETHER INC | Washington | L81 Housing & Shelter | $24.3M | 7 |
| AMERICAN SOCIETY OF ASSOCIATION | Washington | — | $24.3M | 11 |
| AMERICAN ASSOCIATION OF STATE COLLEGES | Washington | B40C Education | $24.2M | 12 |
| Rape Abuse & Incest National Network | Washington | F40 Mental Health & Crisis Intervention | $24.2M | 8 |
| EQUAL JUSTICE WORKS | Washington | I110 Crime & Legal-Related | $24M | 6 |
| National Main Street Center Inc | Washington | S20 Community Improvement & Capacity Building | $23.9M | 7 |
| LEADINGAGE INC | Washington | L030 Housing & Shelter | $23.9M | 16 |
| WASHINGTON HUMANE SOCIETY | Washington | D200 Animal-Related | $23.8M | 6 |
| ASSOCIATION FOR DIAGNOSTICS & LABORATORY | Washington | B99Z Education | $23.8M | 15 |
| THE ALLIANCE FOR CLIMATE PROTECTION | Washington | C30 Environment | $23.8M | 11 |
| ACCION INTERNATIONAL | Washington | Q320 International, Foreign Affairs & National Security | $23.8M | 15 |
| INTERNATIONAL CRISIS GROUP | Washington | Q70Z International, Foreign Affairs & National Security | $23.5M | 9 |
| NATIONAL INSTITUTE OF BUILDING SCIENCES | Washington | M20Z Public Safety, Disaster Preparedness & Relief | $23.4M | 8 |
| FOOD & FRIENDS INC | Washington | G81Z Diseases, Disorders & Medical Disciplines | $23.4M | 9 |
| CERTIFIED AUTOMOTIVE PARTS ASSOCIATION | Washington | — | $23.3M | 1 |
| VITAL VOICES GLOBAL PARTNERSHIP INC | Washington | Q300 International, Foreign Affairs & National Security | $23.2M | 9 |
| CENTER FOR NONPROFIT ADVANCEMENT | Washington | — | $23.2M | 3 |
| NORTH AMERICAN SECURITIES ADMINISTRATORS | Washington | Z99Z Unknown | $23.1M | 7 |
| NATIONAL LOW INCOME HOUSING COALITION | Washington | L99Z Housing & Shelter | $23.1M | 6 |
| WAY TO RISE | Washington | B82 Education | $23M | 0 |
| NPR FOUNDATION | Washington | — | $23M | 5 |
| INSTITUTE OF SCRAP RECYCLING INDUSTRIES | Washington | — | $22.9M | 14 |
| BLACK ECONOMIC ALLIANCE FOUNDATION | Washington | T11 Philanthropy, Voluntarism & Grantmaking Foundations | $22.9M | 2 |
| CONSUMER HEALTHCARE PRODUCTS ASSOCIATION | Washington | — | $22.9M | 13 |
| THE MAYA ANGELOU PUBLIC CHARTER | Washington | B25 Education | $22.8M | 9 |
