| BAYSTATE MEDICAL PRACTICES INC | Springfield | E190 Health Care | $444.9M | 30 |
| BERKLEE COLLEGE OF MUSIC INC | Boston | B420 Education | $441.4M | 18 |
| Williams College | Williamstown | B420 Education | $424.1M | 20 |
| WINCHESTER HOSPITAL | Charlestown | E20 Health Care | $412.2M | 23 |
| SEVEN HILLS FOUNDATION INC & AFFILIATES | Worcester | P820 Human Services | $404.2M | 14 |
| BETH ISRAEL DEACONESS HOSPITAL-PLYMOUTH | Charlestown | E220 Health Care | $389.6M | 27 |
| EMERSON HOSPITAL | Concord | E220 Health Care | $387.3M | 11 |
| WELLESLEY COLLEGE | Wellesley | B420 Education | $385.6M | 15 |
| ELDERHOSTEL INC | Boston | N500 Recreation & Sports | $383.7M | 12 |
| Brockton Hospital Inc | Brockton | E220 Health Care | $381.8M | 13 |
| AMHERST COLLEGE TRUSTEES | Amherst | B420 Education | $379.3M | 19 |
| ACTION FOR BOSTON COMMUNITY DEVELOPMENT | Boston | P200 Human Services | $377.8M | 10 |
| LAHEY CLINIC INC | Charlestown | E300 Health Care | $376.7M | 36 |
| CHA GENERAL SERVICES INC | Malden | E21 Health Care | $369.1M | 13 |
| JSI RESEARCH & TRAINING INSTITUTE INC | Boston | — | $364.9M | 9 |
| MOUNT AUBURN HOSPITAL | Charlestown | E220 Health Care | $359.7M | 26 |
| METROPOLITAN BOSTON HOUSING PARTNERSHIP | Boston | L20 Housing & Shelter | $359.4M | 6 |
| TUFTS MEDICAL CENTER PHYSICIANS | Boston | — | $349.2M | 36 |
| BENTLEY UNIVERSITY | Waltham | B500 Education | $347.7M | 19 |
| WOODS HOLE OCEANOGRAPHIC INSTITUTION | Woods Hole | U210 Science & Technology | $345.9M | 11 |
| BABSON COLLEGE | Babson Park | B420 Education | $344M | 10 |
| MCPHS UNIVERSITY | Boston | B500 Education | $342.3M | 15 |
| NORTH ATLANTIC STATES CARPENTERS HEALTH | Wilmington | — | $339.1M | 6 |
| SUFFOLK UNIVERSITY | Boston | B430 Education | $339M | 19 |
| Children's Medical Center Corporation | Boston | E240 Health Care | $338.4M | 25 |
| EXETER HOSPITAL INC | Charlestown | E220 Health Care | $336M | 23 |
| Lawrence General Hospital | Lawrence | E220 Health Care | $331.9M | 10 |
| EMERSON COLLEGE | Boston | B42 Education | $331.8M | 10 |
| Dental Service of Massachusetts Inc | Boston | — | $329.4M | 10 |
| HARVARD BUSINESS SCHOOL PUBLISHING | Brighton | A330 Arts, Culture & Humanities | $319M | 22 |
| THE US CHARITABLE GIFT TRUST | Boston | T122 Philanthropy, Voluntarism & Grantmaking Foundations | $311.9M | 5 |
| HANSJORG WYSS INSTITUTE FOR BIOLOGICALLY | Boston | B11 Education | $310.9M | 16 |
| SOUTHCOAST PHYSICIANS GROUP INC | Fairhaven | E22 Health Care | $308.5M | 17 |
| TRUSTEES OF THE COLLEGE OF | Worcester | B420 Education | $308.2M | 15 |
| CareQuest Institute for Oral Health Inc | Boston | E60 Health Care | $297.9M | 18 |
| Cummings Foundation Inc | Woburn | T23 Philanthropy, Voluntarism & Grantmaking Foundations | $297.5M | 4 |
| STURDY MEMORIAL HOSPITAL INC | Attleboro | E220 Health Care | $296.2M | 15 |
| UMASS MEMORIAL HEALTH - MILFORD REGIONAL MEDICAL CENTER INC | Milford | E220 Health Care | $292.8M | 10 |
| BETH ISRAEL LAHEY HEALTH PHARMACY INC | Charlestown | E60 Health Care | $292.5M | 20 |
| MERRIMACK COLLEGE | North Andover | B420 Education | $291.2M | 10 |
| BNY MELLON CHARITABLE GIFT FUND | Boston | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $284.9M | 0 |
| THE TRUSTEES OF MOUNT HOLYOKE COLLEGE | South Hadley | B420 Education | $283.9M | 20 |
| SIGHTSAVERS INTERNATIONAL INC | Boston | G41 Diseases, Disorders & Medical Disciplines | $281.3M | 0 |
| FIDELITY NON-PROFIT MANAGEMENT | Boston | S500 Community Improvement & Capacity Building | $279.6M | 12 |
| BMC HEALTH SYSTEM INC | Boston | E11 Health Care | $275.2M | 5 |
| MELROSEWAKEFIELD HEALTHCARE INC | Medford | E22 Health Care | $267.6M | 18 |
| JUSTICE RESOURCE INSTITUTE INC | Needham | I40 Crime & Legal-Related | $266.5M | 11 |
| Holyoke Medical Center Inc | Holyoke | E220 Health Care | $266.1M | 9 |
| ISO NEW ENGLAND INC | Holyoke | C35 Environment | $264.6M | 37 |
| ASSOCIATED PHYSICIANS OF HARVARD MEDICAL | Boston | E12 Health Care | $258M | 12 |
