| GRHS FOUNDATION INC | Rochester | S50Z Community Improvement & Capacity Building | $46M | 10 |
| McAuley Seton Home Care | Buffalo | E99I Health Care | $45.9M | 22 |
| The Sundheim Family Foundation | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $45.8M | 0 |
| PSC-CUNY WELFARE FUND | New York | — | $45.8M | 8 |
| HORIZON HEALTH SERVICES INC | Getzville | F20Z Mental Health & Crisis Intervention | $45.8M | 7 |
| PATHSTONE CORPORATION | Rochester | S32Z Community Improvement & Capacity Building | $45.7M | 7 |
| THE YALE CLUB OF NEW YORK CITY | New York | — | $45.6M | 6 |
| CENTER FOR DEVELOPMENTAL DISABILITIES | Woodbury | P820 Human Services | $45.6M | 10 |
| FAMILY LIFE ACADEMY CHARTER SCHOOLS | Bronx | B29 Education | $45.5M | 15 |
| HAMASPIK OF ORANGE COUNTY INC | Monroe | — | $45.4M | 7 |
| FALLON HEALTH WEINBERG INC | Getzville | P75 Human Services | $45.4M | 11 |
| LIGHTHOUSE GUILD INTERNATIONAL INC | New York | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $45.4M | 17 |
| Stony Brook Radiology University Faculty Practice Corporation | Stony Brook | E20 Health Care | $45.4M | 8 |
| NEW YORK FOUNDATION FOR SENIOR CITIZENS | New York | — | $45.4M | 5 |
| LOCAL 338 RWDSUUFCW HEALTH & WELFARE | Mineola | — | $45.4M | 10 |
| SENIOR CARE CONNECTION INC | Schenectady | — | $45.4M | 15 |
| CHILDREN'S HOME OF WYOMING CONFERENCE | Binghamton | P700 Human Services | $45.3M | 7 |
| ZEARN | New York | B19 Education | $45.3M | 7 |
| WOMEN'S LEAGUE COMMUNITY RESIDENCES | Brooklyn | — | $45.3M | 8 |
| JEWISH HOME LIFECARE SARAH NEUMAN CENTER | New York | E910 Health Care | $45.2M | 8 |
| LOCAL 295 IBT EMPLOYER GROUP | New York | — | $45.2M | 3 |
| CROUSE MEDICAL PRACTICE PLLC | Syracuse | E30 Health Care | $45.1M | 9 |
| FOUNDATION FOR THE ELDERLY | Inwood | P81Z Human Services | $45.1M | 5 |
| NORTH SHORE ANIMAL LEAGUE AMERICA INC | Port Washington | D200 Animal-Related | $45.1M | 11 |
| NEW YORK CITY TOURISM CONVENTIONS | New York | — | $45M | 9 |
| LAKEVILLE SURGERY PC | Westbury | E60 Health Care | $45M | 5 |
| HOTEL AND GAMING TRADES COUNCIL AFL-CIO | New York | J40 Employment | $45M | 25 |
| National Health Care Inc and Affiliate | Valley Stream | — | $44.9M | 0 |
| CHAPEL & YORK US FOUNDATION INC | New York | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $44.8M | 0 |
| THE POSSE FOUNDATION INC | New York | B82 Education | $44.8M | 9 |
| THE MARK FOUNDATION FOR CANCER RESEARCH | New York | G30 Diseases, Disorders & Medical Disciplines | $44.8M | 10 |
| THE WINSTON PREPARATORY SCHOOL | New York | B28Z Education | $44.7M | 15 |
| PETER G PETERSON FOUNDATION | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $44.6M | 6 |
| CENTRAL NASSAU GUIDANCE AND COUNSELING | Hicksville | F300 Mental Health & Crisis Intervention | $44.6M | 11 |
| CARSON FAMILY CHARITABLE TRUST | New York | — | $44.6M | 3 |
| ST JOHNLAND NURSING CENTER INC | Kings Park | E91Z Health Care | $44.5M | 7 |
| UNITED NATIONS DEVELOPMENT CORPORATION | New York | Q420 International, Foreign Affairs & National Security | $44.3M | 7 |
| Little Red School House INC | New York | — | $44.2M | 8 |
| CLASSICAL CHARTER SCHOOLS | Bronx | B29 Education | $44.2M | 8 |
| NATIONAL CENTER FOR CIVIC INNOVATION IN | New York | S43 Community Improvement & Capacity Building | $44.2M | 4 |
| QUEENS COMMUNITY HOUSE | Forest Hills | P280 Human Services | $44.2M | 8 |
| HAWTHORNDEN FOUNDATION | New York | B82 Education | $44.1M | 3 |
| AABR INC | College Point | P73 Human Services | $44.1M | 5 |
| ANDERSON CENTER SERVICES | Staatsburg | — | $44.1M | 8 |
| JAZZ AT LINCOLN CENTER INC | New York | A680 Arts, Culture & Humanities | $44M | 15 |
| NEW YORK STATE PUBLIC EMPLOYEES | Albany | — | $44M | 11 |
| NATIONAL HOCKEY LEAGUE HEALTH FUND | New York | Y43 Mutual & Membership Benefit | $44M | 0 |
| TRANSITIONAL SERVICES OF NEW YORK INC | Whitestone | P72Z Human Services | $43.9M | 8 |
| HOMES FOR THE HOMELESS INC | New York | P850 Human Services | $43.8M | 5 |
| CRIMINAL JUSTICE REFORM FOUNDATION | New York | I50 Crime & Legal-Related | $43.8M | 9 |
