| WOOSTER COMMUNITY HOSPITAL FOUNDATION | Wooster | E19 Health Care | $39.2M | 8 |
| United Way of Greater Cincinnati | Cincinnati | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $39.1M | 8 |
| COLUMBUS CENTER FOR HUMAN SERVICES INC | Columbus | P730 Human Services | $39.1M | 5 |
| Greene Memorial Hospital Inc | Miamisburg | E220 Health Care | $39M | 21 |
| UNION CONSTRUCTION WORKERS HEALTH PLAN | Holland | — | $38.9M | 1 |
| Bowling Green State University Foundation Inc | Bowling Green | B110 Education | $38.6M | 7 |
| UNITED CHURCH INSURANCE COMPANY | Cleveland | Y20 Mutual & Membership Benefit | $38.5M | 4 |
| Lifeline of Ohio Organ Procurement Inc | Columbus | E65Z Health Care | $38.4M | 10 |
| COMMUNITY ACTION COMMITTEE OF | Piketon | P200 Human Services | $38.3M | 7 |
| PIPEFITTERS UNION HEALTH & WELFARE | Cincinnati | Y43 Mutual & Membership Benefit | $38.3M | 3 |
| TWIN LAKES | Cincinnati | L22 Housing & Shelter | $38.2M | 4 |
| Circle Health Services | Cleveland | — | $38.1M | 7 |
| WILMINGTON COLLEGE | Wilmington | B420 Education | $37.9M | 8 |
| INTERNATIONAL BROTHERHOOD OF ELECTRICAL WORKERS LOCAL 38 HEALTH & WELFARE F | Cleveland | — | $37.8M | 0 |
| LIGHTHOUSE YOUTH SERVICES | Cincinnati | P300 Human Services | $37.4M | 7 |
| AREA OFFICE ON AGING OF | Toledo | P810 Human Services | $37.3M | 6 |
| OHIO BANKERS BENEFITS TRUST | Columbus | — | $37.2M | 1 |
| LOURDES UNIVERSITY | Sylvania | B420 Education | $37M | 11 |
| CINCINNATI NATURE CENTER | Milford | C600 Environment | $36.9M | 5 |
| Trust Agreement for the Ohio Farmers | Westfield Center | Y34 Mutual & Membership Benefit | $36.9M | 0 |
| POSITIVE EDUCATION PROGRAM | Cleveland | F330 Mental Health & Crisis Intervention | $36.7M | 13 |
| BLANCHARD VALLEY CONTINUING CARE SERVICES | Findlay | L22 Housing & Shelter | $36.7M | 9 |
| MID-AMERICAN ATHLETIC CONFERENCE INC | Cleveland | — | $36.6M | 6 |
| THE SEVEN HILLS SCHOOL | Cincinnati | B250 Education | $36.5M | 11 |
| BUTLER RURAL ELECTRIC COOP INC | Oxford | — | $36.5M | 15 |
| SOUTHWEST GENERAL MEDICAL GROUP INC | Middleburg Heights | E30Z Health Care | $36.4M | 10 |
| COLUMBUS SYMPHONY ORCHESTRA | Columbus | A690 Arts, Culture & Humanities | $36.3M | 0 |
| Hiram College | Hiram | B420 Education | $36M | 7 |
| LUTHERAN HOMES SOCIETY INC | Toledo | P40 Human Services | $35.7M | 9 |
| COMMUNICARE HEALTH BENEFIT TRUST | Cincinnati | Y43 Mutual & Membership Benefit | $35.7M | 0 |
| OHIO CAPITAL CORPORATION FOR HOUSING | Columbus | L190 Housing & Shelter | $35.5M | 24 |
| KIPP COLUMBUS FOUNDATION | Columbus | B12 Education | $35.4M | 0 |
| NATIONAL FOOTBALL MUSEUM INC | Canton | A500 Arts, Culture & Humanities | $35.3M | 10 |
| LIFEWISE INC | Hilliard | O50 Youth Development | $35.3M | 5 |
| LAKE ERIE COLLEGE | Painesville | B430 Education | $35M | 12 |
| HATHAWAY BROWN SCHOOL | Shaker Heights | B200 Education | $35M | 6 |
| HENRY COUNTY HOSPITAL INC | Napoleon | E22Z Health Care | $34.9M | 9 |
| PELOTONIA | Columbuus | B11 Education | $34.7M | 7 |
| TWIN TOWERS | Cincinnati | P750 Human Services | $34.7M | 4 |
| NORTH CENTRAL ELECTRIC COOPERATIVE INC | Attica | — | $34.5M | 19 |
| PIKE HEALTH SERVICES INC | Waverly | E99Z Health Care | $34.4M | 12 |
| YOUNGSTOWN STATE UNIVERSITY FOUNDATION | Youngstown | B11 Education | $34.4M | 4 |
| THE CORPORATION FOR OHIO APPALACHIAN | Athens | S200 Community Improvement & Capacity Building | $34.2M | 1 |
| Kettering Adventist Healthcare | Miamisburg | — | $34.1M | 18 |
| Strategies to End Homelessness Inc | Cincinnati | P85 Human Services | $34M | 2 |
| Bluffton University | Bluffton | B420 Education | $33.9M | 5 |
| HOSPICE OF NORTHWEST OHIO | Perrysburg | — | $33.8M | 8 |
| Greenacres Foundation Inc | Cincinnati | C32 Environment | $33.8M | 3 |
| THE FOODBANK INC | Dayton | K31 Food, Agriculture & Nutrition | $33.7M | 3 |
| URSULINE COLLEGE | Pepper Pike | B420 Education | $33.7M | 8 |
