| CINCINNATI HILLS CHRISTIAN ACADEMY | Cincinnati | B200 Education | $33.6M | 7 |
| WILLOW BROOK CHRISTIAN SERVICES | Delaware | L22 Housing & Shelter | $33.6M | 7 |
| JENNINGS CENTER FOR OLDER ADULTS | Garfield Heights | E910 Health Care | $33.6M | 7 |
| UNIVERSITY OF RIO GRANDE | Rio Grande | B430 Education | $33.5M | 10 |
| PREVAILING WAGE CONTRACTORS | Columbus | — | $33.4M | 1 |
| CLEVELAND INSTITUTE OF MUSIC | Cleveland | B500 Education | $33.4M | 12 |
| NORTHWESTERN OHIO PLUMBERS & PIPEFI | Northwood | — | $33.2M | 0 |
| UNION BENEFITS TRUST | Westerville | — | $32.9M | 0 |
| COLUMBUS STATE COMMUNITY COLLEGE | Columbus | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $32.6M | 0 |
| COLUMBUS NEIGHBORHOOD HEALTH CENTER INC | Columbus | E32 Health Care | $32.6M | 10 |
| Koinonia Homes Inc | Independence | P73Z Human Services | $32.5M | 12 |
| LUTHERAN SOCIAL SERVICES OF CENTRAL OHIO | Columbus | — | $32.3M | 8 |
| LIMA MEMORIAL PROFESSIONAL CORPORATION | Lima | E30 Health Care | $32.3M | 18 |
| MARYHAVEN INC | Columbus | F22Z Mental Health & Crisis Intervention | $32.2M | 14 |
| Ohio Shared Information Services | Cincinnati | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $32.1M | 10 |
| TOURNAMENTS FOR CHARITY | Dublin | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $31.9M | 0 |
| SCHOTTENSTEIN STORES CORPORATION | Columbus | — | $31.9M | 1 |
| Hospice of the Western Reserve at University | Cleveland | — | $31.9M | 6 |
| DILEY RIDGE MEDICAL CENTER | Columbus | E20 Health Care | $31.5M | 14 |
| YOUNG MENS CHRISTIAN ASSOCIATION OF CLEVELAND | Cleveland | P270 Human Services | $31.3M | 7 |
| Jewish Federation of Cincinnati | Cincinnati | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $31.2M | 9 |
| HOSPICE OF CENTRAL OHIO | Newark | — | $31.2M | 10 |
| WOOD COUNTY SCHOOLS BENEFIT PLAN | Pemberville | — | $31M | 0 |
| CINCINNATI COUNTRY DAY SCHOOL | Cincinnati | B200 Education | $30.9M | 8 |
| Judson | Cleveland | P750 Human Services | $30.7M | 8 |
| HEBREW ACADEMY OF CLEVELAND | Cleveland Heights | — | $30.6M | 5 |
| CATHOLIC SOCIAL SERVICES | Dayton | — | $30.6M | 3 |
| CLARK COUNTY HOSPITAL SERVICES | Dayton | E24 Health Care | $30.6M | 0 |
| NEIGHBORHOOD HEALTH CARE INC | Cleveland | E320 Health Care | $30.3M | 7 |
| EASTWAY CORPORATION | Dayton | F300 Mental Health & Crisis Intervention | $29.9M | 11 |
| THE UNIVERSITY OF TOLEDO FOUNDATION | Toledo | B110 Education | $29.9M | 9 |
| LAUREL LAKE RETIREMENT COMMUNITY INC | Hudson | A6B Arts, Culture & Humanities | $29.9M | 8 |
| OHIO HISTORICAL SOCIETY | Columbus | A800 Arts, Culture & Humanities | $29.9M | 5 |
| COLUMBUS ASSOCIATION FOR THE PERFORMING | Columbus | A610 Arts, Culture & Humanities | $29.9M | 8 |
| MENTAL HEALTH SERVICES FOR HOMELESS | Cleveland | P85Z Human Services | $29.8M | 7 |
| Trust for the Post Employment Health Plan for | Columbus | — | $29.6M | 0 |
| EDISON WELDING INSTITUTE | Columbus | J220 Employment | $29.5M | 15 |
| WRIGHT STATE PHYSICIANS INC | Fairborn | E22Z Health Care | $29.3M | 15 |
| HOLZER MEDICAL CENTER - JACKSON | Gallipolis | E220 Health Care | $29.1M | 8 |
| FOUNDATION FOR APPALACHIAN OHIO | Nelsonville | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $29M | 2 |
| KENDAL AT OBERLIN | Oberlin | P750 Human Services | $28.9M | 9 |
| OUCU FINANCIAL CREDIT UNION | Athens | — | $28.8M | 16 |
| PORTSMOUTH HOSPITAL CORPORATION | Portsmouth | E22 Health Care | $28.7M | 11 |
| ROCKING HORSE CHILDREN'S HEALTH CENTER | Springfield | E30 Health Care | $28.6M | 9 |
| ASHTABULA COUNTY MEDICAL CENTER | Ashtabula | — | $28.5M | 10 |
| Trust For The City of Phoenix Arizona Post | Columbus | Y44 Mutual & Membership Benefit | $28.5M | 0 |
| Life Connection of Ohio | Maumee | E65Z Health Care | $28.4M | 10 |
| SUNSET RETIREMENT COMMUNITIES INC | Toledo | P750 Human Services | $28.2M | 11 |
| THE CONVENTION AND VISITORS BUREAU OF GREATER CLEVELAND INC | Cleveland | — | $28.2M | 9 |
| KEYBANK FOUNDATION | Cleveland | — | $28.1M | 0 |
