| PRO PUBLICA INC | New York | A20 Arts, Culture & Humanities | $64.3M | 11 |
| RYE COUNTRY DAY SCHOOL | Rye | B200 Education | $64.2M | 9 |
| NORTHWELL HEALTH STERN FAMILY CENTER FOR REHA | Westbury | E910 Health Care | $64M | 20 |
| THE BERKELEY CARROLL SCHOOL | Brooklyn | B200 Education | $63.9M | 13 |
| HOUSING WORKS INC | Brooklyn | S41Z Community Improvement & Capacity Building | $63.8M | 8 |
| New York Municipal Power Agency | East Syracuse | Y22 Mutual & Membership Benefit | $63.6M | 1 |
| CHAUTAUQUA INSTITUTION | Chautauqua | A610 Arts, Culture & Humanities | $63.4M | 21 |
| COMPASSION COALITION INC | Frankfort | P20 Human Services | $63.3M | 4 |
| APOLLO OPPORTUNITY FOUNDATION | New York | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $63.1M | 0 |
| THE SPENCE SCHOOL | New York | B200 Education | $63.1M | 12 |
| Ludwig Institute for Cancer Research Ltd | New York | — | $63M | 20 |
| RELAY GRADUATE SCHOOL OF EDUCATION | New York | B40 Education | $62.8M | 7 |
| Stony Brook Anaesthesiology University Faculty Practice Corporation | Stony Brook | E20 Health Care | $62.8M | 9 |
| HOSPICE CARE NETWORK | Woodbury | P740 Human Services | $62.8M | 20 |
| THE KALLIR FAMILY FOUNDATION | New York | A51 Arts, Culture & Humanities | $62.7M | 0 |
| GLENN GREENBERG AND LINDA VESTER FOUNDATION INC | New York | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $62.7M | 0 |
| The End Fund Inc | New York | Q30 International, Foreign Affairs & National Security | $62.7M | 8 |
| HEALTH REIMBURSEMENT ACCOUNT FUND OF THE ELECTRICAL INDUSTRY | Flushing | Y43 Mutual & Membership Benefit | $62.6M | 0 |
| PLEDGER CHARITABLE LTD | Brooklyn | — | $62.6M | 1 |
| Motor Vehicle Accident Indemnification Corporation | New York | — | $62.6M | 10 |
| THE SILVERCREST CENTER FOR NURSING AND REHABILITATION | Jamaica | E910 Health Care | $62.6M | 21 |
| NYSARC INC MONROE COUNTY CHAPTER | Rochester | F330 Mental Health & Crisis Intervention | $62.5M | 8 |
| HACKLEY SCHOOL | Tarrytown | E200 Health Care | $62.5M | 8 |
| NFL FOUNDATION INC | New York | N120 Recreation & Sports | $62.5M | 0 |
| METROPOLITAN JEWISH HEALTH SYSTEM INC | New York | E19 Health Care | $62.4M | 8 |
| New York Historical Society | New York | A800 Arts, Culture & Humanities | $62.3M | 15 |
| THE FRIENDS OF CROWN HEIGHTS EDUCATIONAL CENTERS INC | Brooklyn | P330 Human Services | $62.3M | 8 |
| TANSY CHARITABLE FOUNDATION C/O ERIC WEPSIC | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $62.3M | 0 |
| HAO FAMILY FOUNDATION | Saratoga Springs | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $62.2M | 0 |
| ST JAMES HOSPITAL | Hornell | E220 Health Care | $62M | 11 |
| WILDWOOD PROGRAMS INC | Latham | Z99 Unknown | $61.9M | 2 |
| HISPANIC FEDERATION INC | New York | S50Z Community Improvement & Capacity Building | $61.8M | 9 |
| ALBANY COLLEGE OF PHARMACY AND HEALTH SCIENCES | Albany | B500 Education | $61.8M | 8 |
| SECURITIES INDUSTRY AND FINANCIAL MARKETS ASSOCIATION | New York | S40 Community Improvement & Capacity Building | $61.8M | 13 |
| THE JEWISH ASSOCIATION FOR SERVICES FOR THE AGED | New York | P810 Human Services | $61.7M | 7 |
| COMMUNITY MEMORIAL HOSPITAL INC | Hamilton | E220 Health Care | $61.6M | 9 |
| CONCERN FOR INDEPENDENT LIVING INC | Medford | F330 Mental Health & Crisis Intervention | $61.5M | 8 |
| THE JAMES AND JUDITH K DIMON FOUNDATION | New York | T20J Philanthropy, Voluntarism & Grantmaking Foundations | $61.4M | 0 |
| AMNESTY INTERNATIONAL OF THE USA INC | New York | Q700 International, Foreign Affairs & National Security | $61.2M | 13 |
| NEW YORK SHAKESPEARE FESTIVAL | New York | A650 Arts, Culture & Humanities | $61.1M | 13 |
| WESTERN NEW YORK INDEPENDENT LIVING INC | Buffalo | E99Z Health Care | $61.1M | 3 |
| INDEPENDENT LIVING ASSOCIATION | Brooklyn | P700 Human Services | $61M | 8 |
| THE PERPETUAL CARE FUND ADMINISTERED BY THE GREEN-WOOD CEMETERY AS TRUSTEE | Brooklyn | — | $61M | 10 |
| DOMINICAN UNIVERSITY NEW YORK | Orangeburg | — | $60.9M | 10 |
| EVERYTOWN FOR GUN SAFETY ACTION FUND INC | New York | I192 Crime & Legal-Related | $60.7M | 6 |
| THE VIVIAN BEAUMONT THEATERINC D/B/A LINCOLN CENTER THEATER | New York | A650 Arts, Culture & Humanities | $60.6M | 15 |
| SHED NYC INC | New York | A20 Arts, Culture & Humanities | $60.6M | 19 |
| GLOBAL POVERTY PROJECT INC | New York | V30 Social Science | $60.6M | 12 |
| Harmony Health Care Long Island | Garden City | E30 Health Care | $60.5M | 11 |
| NEW YORK LIFE INSURANCE COMPANY EMPLOYEES' HEALTH AND LIFE BENEFIT TRUST | New York | — | $60.4M | 0 |
