| School-Based Health Alliance | Washington | E80 Health Care | $35.8M | 7 |
| AMERICAN INDEPENDENT MEDIA | Washington | A30 Arts, Culture & Humanities | $35.7M | 2 |
| DEMOCRACY MATTERS FOUNDATION | Washington | R60 Civil Rights, Social Action & Advocacy | $35.6M | 0 |
| US GRAINS COUNCIL | Washington | — | $35.5M | 13 |
| MED-PROJECT USA | Washington | F20 Mental Health & Crisis Intervention | $35.4M | 0 |
| CLIMATE POWER | Washington | C20 Environment | $35.3M | 7 |
| Bank Policy Institute | Washington | — | $35.3M | 11 |
| INTERNATIONAL UNION OF OPERATING ENGINEERS NATIONAL TRAINING FUND | Washington | J22 Employment | $35.2M | 7 |
| Humane World for Animals International | Washington | D200 Animal-Related | $35.1M | 24 |
| AUTISM SPEAKS INC | Washington | G128 Diseases, Disorders & Medical Disciplines | $35M | 10 |
| THE INTERNATIONAL COUNCIL ON CLEAN TRANSPORTATION INC | Washington | C052 Environment | $34.9M | 10 |
| CHIEF EXECUTIVES ORGANIZATION INC | Washington | S41 Community Improvement & Capacity Building | $34.9M | 11 |
| AMERICAN DENTAL EDUCATION ASSOCIATION | Washington | B50 Education | $34.9M | 13 |
| CRUISE LINES INTERNATIONAL ASSOCIATION INC | Washington | — | $34.7M | 11 |
| OCEANA INC | Washington | C013 Environment | $34.7M | 11 |
| RESOLVE UGANDA INCORPORATED | Washington | Q33 International, Foreign Affairs & National Security | $34.5M | 0 |
| Conservative Partnership Institute | Washington | B60 Education | $34.5M | 8 |
| TRANSPORT WORKERS UNION OF AMERICA | Washington | — | $34.4M | 25 |
| CAMPAIGN FOR TOBACCO-FREE KIDS | Washington | F520 Mental Health & Crisis Intervention | $34.3M | 14 |
| INSTITUTE OF INTERNATIONAL FINANCE | Washington | — | $34.2M | 14 |
| LABORERS' NATIONAL HEALTH & WELFARE FUND | Washington | — | $34M | 5 |
| NATIONAL SOCIETY DAUGHTERS OF THE AMERICAN REVOLUTION | Washington | A800 Arts, Culture & Humanities | $33.9M | 9 |
| BAINUM FAMILY FOUNDATION | Washington | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $33.8M | 3 |
| USAGING | Washington | — | $33.7M | 10 |
| THE HOSPITAL FOR SICK CHILDREN | Washington | E240 Health Care | $33.7M | 11 |
| Recording Industry Association Of America Inc | Washington | — | $33.6M | 11 |
| SOLAR ENERGY INDUSTRIES ASSOCIATION | Washington | — | $33.6M | 14 |
| COMMUNITY CONNECTIONS INC | Washington | F32Z Mental Health & Crisis Intervention | $33.6M | 9 |
| THE FAIRNESS PROJECT | Washington | L50 Housing & Shelter | $33.3M | 6 |
| STATES UNITED DEMOCRACY CENTER INC | Washington | R01 Civil Rights, Social Action & Advocacy | $33.3M | 7 |
| PROTECT DEMOCRACY PROJECT | Washington | R05 Civil Rights, Social Action & Advocacy | $33.1M | 8 |
| Foundation for the Defense of Democracies Inc | Washington | Q01 International, Foreign Affairs & National Security | $32.5M | 11 |
| NATIONAL CONFECTIONERS ASSOCIATION OF THE UNITED STATES INC | Washington | — | $32.5M | 9 |
| UNRWA USA NATIONAL COMMITTEE INC | Washington | Q33 International, Foreign Affairs & National Security | $32.4M | 4 |
| HOUSING COUNSELING SERVICES INC | Washington | L80Z Housing & Shelter | $32.4M | 7 |
| COUNCIL FOR ADVANCEMENT AND SUPPORT OF EDUCATION | Washington | — | $32M | 12 |
| America First Legal Foundation | Washington | I83 Crime & Legal-Related | $32M | 7 |
| CAROL AND GENE LUDWIG FAMILY FOUNDATION | Washington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $32M | 0 |
| NATIONAL SPECTRUM CONSORTIUM INC | Washington | U01 Science & Technology | $31.9M | 1 |
| INTERNATIONAL SPY MUSEUM | Washington | A50 Arts, Culture & Humanities | $31.8M | 7 |
| JOURNALISM DEVELOPMENT NETWORK INC | Washington | B60 Education | $31.7M | 8 |
| MANAGED FUNDS ASSOCIATION | Washington | — | $31.7M | 11 |
| AMERICAN ASSOCIATION FOR JUSTICE | Washington | — | $31.7M | 10 |
| COOPERATIVE LEAGUE OF THE UNITED STATES OF AMERICA | Washington | — | $31.6M | 8 |
| THE FIELD SCHOOL INC | Washington | — | $31.5M | 8 |
| WASHINGTON HEALTH INSTITUTE | Washington | E30 Health Care | $31.5M | 4 |
| GENERATION YOU EMPLOYED INC | Washington | O50 Youth Development | $31.4M | 10 |
| NATIONAL ASS FOR EQUAL OPPORTUNITY IN HIGHER EDUCATION | Washington | — | $31.4M | 1 |
| MARTHA'S TABLE INC | Washington | K300 Food, Agriculture & Nutrition | $31.3M | 8 |
| CONGRESSIONAL BLACK CAUCUS FOUNDATION INC | Washington | R22Z Civil Rights, Social Action & Advocacy | $31.3M | 6 |
