| THE DISTRICT OF COLUMBIA BAR FOUNDATION | Washington | I128 Crime & Legal-Related | $42.3M | 5 |
| US Chamber of Commerce Foundation | Washington | B05 Education | $42.3M | 15 |
| AMERICAN GAS ASSOCIATION | Washington | — | $42.1M | 11 |
| THE WILDERNESS SOCIETY | Washington | C340 Environment | $42M | 14 |
| S & R Foundation | Washington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $41.4M | 0 |
| NATIONAL WOMEN'S LAW CENTER | Washington | — | $41.4M | 18 |
| THE RECYCLING PARTNERSHIP INC | Washington | C27 Environment | $41.2M | 7 |
| EUPHEMIA L HAYNES PUBLIC CHARTER SCHOOL INC | Washington | B29 Education | $41M | 9 |
| NATIONAL COUNCIL OF ARCHITECTURAL REGISTRATION BOARDS | Washington | — | $40.9M | 24 |
| SEARCH FOR COMMON GROUND | Washington | Q400 International, Foreign Affairs & National Security | $40.9M | 13 |
| National Business Aviation Association Inc | Washington | — | $40.9M | 16 |
| THE DC CENTRAL KITCHEN INC | Washington | — | $40.4M | 7 |
| FORAFRIKA USA | Washington | X99Z Religion-Related | $40.2M | 1 |
| GREENPEACE INC | Washington | — | $40.2M | 15 |
| NATIONAL TREASURY EMPLOYEES UNION | Washington | — | $40.2M | 7 |
| AMERICAN PHARMACISTS ASSOCIATION | Washington | — | $40.1M | 21 |
| AMERICAN PUBLIC TRANSPORTATION ASSOCIATION | Washington | — | $39.4M | 13 |
| CARLOS ROSARIO INTERNATIONAL PUBLIC CHARTER SCHOOL INC | Washington | B60 Education | $39M | 6 |
| ANIC PROTECTED CELL OF LTC RE | Washington | S40 Community Improvement & Capacity Building | $38.8M | 0 |
| AFRICAN WILDLIFE FOUNDATION INC | Washington | D30Z Animal-Related | $38.6M | 17 |
| WASHINGTON INSTITUTE FOR EDUCATION AND R | Washington | B50 Education | $38.5M | 6 |
| THE GERMAN MARSHALL FUND OF THE UNITED STATES | Washington | Q050 International, Foreign Affairs & National Security | $38.4M | 12 |
| GRAND CHAPTER OF THE DELTA SIGMA THETA SORORITY INC | Washington | — | $38.3M | 11 |
| NATIONAL ASSOCIATION OF SOCIAL WORKERS | Washington | — | $38.2M | 11 |
| AMERICAN JOURNALISM PROJECT INC | Washington | A30 Arts, Culture & Humanities | $38.1M | 9 |
| BIPARTISAN POLICY CENTER INC | Washington | W052 Public & Societal Benefit | $37.8M | 14 |
| REWIRING AMERICA | Washington | C35 Environment | $37.6M | 7 |
| LUTHERAN SOCIAL SERVICES NATIONAL CAPITAL AREAINC | Washington | P75Z Human Services | $37.5M | 9 |
| FWDUS INC | Washington | R01 Civil Rights, Social Action & Advocacy | $37.5M | 9 |
| CENTER FOR VOTER INFORMATION | Washington | R24 Civil Rights, Social Action & Advocacy | $37.4M | 5 |
| CENTER FOR AMERICAN PROGRESS | Washington | R05 Civil Rights, Social Action & Advocacy | $37.4M | 9 |
| NATIONAL ASSOCIATION OF REAL ESTATE INVESTMENT TRUSTS (NAREIT) | Washington | S41 Community Improvement & Capacity Building | $37.3M | 12 |
| NEW AMERICA FOUNDATION | Washington | Z99 Unknown | $37.2M | 14 |
| AMERICA FIRST WORKS INC | Washington | R99 Civil Rights, Social Action & Advocacy | $36.7M | 5 |
| AMERICAN FARM BUREAU FEDERATION | Washington | — | $36.7M | 34 |
| AMERICAN RIVERS INC | Washington | C013 Environment | $36.6M | 11 |
| PARTNERSHIP FOR PUBLIC SERVICE INC | Washington | J05 Employment | $36.6M | 10 |
| ENGENDERHEALTH INC | Washington | Q023 International, Foreign Affairs & National Security | $36.5M | 10 |
| INTERNATIONAL FUND FOR ANIMAL WELFARE INC | Washington | D20 Animal-Related | $36.5M | 10 |
| US TRAVEL ASSOCIATION | Washington | — | $36.5M | 11 |
| MARET SCHOOL INC | Washington | B20Z Education | $36.4M | 9 |
| THE EURASIA FOUNDATION | Washington | Q123 International, Foreign Affairs & National Security | $36.4M | 8 |
| AMERICAN COUNCIL ON EDUCATION | Washington | B030 Education | $36.3M | 15 |
| EVIDENCE ACTION | Washington | Q33 International, Foreign Affairs & National Security | $36.2M | 8 |
| ENTERTAINMENT SOFTWARE ASSOCIATION | Washington | — | $36.2M | 11 |
| CAPITAL CITY PUBLIC CHARTER SCHOOL INC | Washington | B24 Education | $36.1M | 6 |
| FUTURES INDUSTRY ASSOCIATION INC | Washington | — | $36.1M | 11 |
| Defenders of Wildlife | Washington | D300 Animal-Related | $36.1M | 18 |
| DELTA SIGMA THETA SORORITY INC COMBINED | Washington | — | $36M | 0 |
| Albert B Sabin Vaccine Institute Inc | Washington | Z99 Unknown | $35.9M | 9 |
