| SOCIETY FOR NEUROSCIENCE | Washington | H030 Medical Research | $41.8M | 11 |
| S & R Foundation | Washington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $41.4M | 0 |
| NATIONAL WOMEN'S LAW CENTER | Washington | — | $41.4M | 18 |
| THE RECYCLING PARTNERSHIP INC | Washington | C27 Environment | $41.2M | 7 |
| The Partnership Project Inc | Washington | C01 Environment | $41.1M | 7 |
| National Business Aviation Association Inc | Washington | — | $40.9M | 16 |
| THE DC CENTRAL KITCHEN INC | Washington | — | $40.4M | 7 |
| FORAFRIKA USA | Washington | X99Z Religion-Related | $40.2M | 1 |
| GREENPEACE INC | Washington | — | $40.2M | 15 |
| NATIONAL TREASURY EMPLOYEES UNION | Washington | — | $40.2M | 7 |
| AMERICAN PHARMACISTS ASSOCIATION | Washington | — | $40.1M | 21 |
| Albert B Sabin Vaccine Institute Inc | Washington | Z99 Unknown | $40.1M | 8 |
| AMERICAN COUNCIL ON EDUCATION | Washington | B030 Education | $39.7M | 14 |
| Building Hope Finance | Washington | B12 Education | $39.4M | 6 |
| CLIMATE AND CLEAN ENERGY EQUITY FUND | Washington | C01 Environment | $39.1M | 0 |
| ANIC PROTECTED CELL OF LTC RE | Washington | S40 Community Improvement & Capacity Building | $38.8M | 0 |
| MARET SCHOOL INC | Washington | B20Z Education | $38.6M | 8 |
| AFRICAN WILDLIFE FOUNDATION INC | Washington | D30Z Animal-Related | $38.6M | 17 |
| WASHINGTON INSTITUTE FOR EDUCATION AND R | Washington | B50 Education | $38.5M | 6 |
| National 4-H Council | Washington | O52Z Youth Development | $38.4M | 12 |
| GRAND CHAPTER OF THE DELTA SIGMA THETA | Washington | — | $38.3M | 11 |
| AMERICAN JOURNALISM PROJECT INC | Washington | A30 Arts, Culture & Humanities | $38.1M | 9 |
| NATIONAL ASSOCIATION OF SOCIAL WORKERS | Washington | — | $37.9M | 8 |
| REWIRING AMERICA | Washington | C35 Environment | $37.6M | 7 |
| LUTHERAN SOCIAL SERVICES | Washington | P75Z Human Services | $37.5M | 9 |
| FWDUS INC | Washington | R01 Civil Rights, Social Action & Advocacy | $37.5M | 9 |
| CENTER FOR VOTER INFORMATION | Washington | R24 Civil Rights, Social Action & Advocacy | $37.4M | 5 |
| NATIONAL ASSOCIATION OF REAL ESTATE | Washington | S41 Community Improvement & Capacity Building | $37.3M | 12 |
| NATIONAL COUNCIL OF ARCHITECTURAL | Washington | — | $36.9M | 25 |
| EUPHEMIA L HAYNES PUBLIC CHARTER | Washington | B29 Education | $36.7M | 9 |
| AMERICAN FARM BUREAU FEDERATION | Washington | — | $36.7M | 34 |
| ENGENDERHEALTH INC | Washington | Q023 International, Foreign Affairs & National Security | $36.5M | 10 |
| INTERNATIONAL FUND FOR ANIMAL WELFARE INC | Washington | D20 Animal-Related | $36.5M | 10 |
| US TRAVEL ASSOCIATION | Washington | — | $36.5M | 11 |
| THE EURASIA FOUNDATION | Washington | Q123 International, Foreign Affairs & National Security | $36.4M | 8 |
| EVIDENCE ACTION | Washington | Q33 International, Foreign Affairs & National Security | $36.2M | 8 |
| ENTERTAINMENT SOFTWARE ASSOCIATION | Washington | — | $36.2M | 11 |
| CAPITAL CITY PUBLIC CHARTER SCHOOL INC | Washington | B24 Education | $36.1M | 6 |
| FUTURES INDUSTRY ASSOCIATION INC | Washington | — | $36.1M | 11 |
| Defenders of Wildlife | Washington | D300 Animal-Related | $36.1M | 18 |
| DELTA SIGMA THETA SORORITY INC | Washington | — | $36M | 0 |
| School-Based Health Alliance | Washington | E80 Health Care | $35.8M | 7 |
| AMERICAN INDEPENDENT MEDIA | Washington | — | $35.7M | 2 |
| DEMOCRACY MATTERS FOUNDATION | Washington | R60 Civil Rights, Social Action & Advocacy | $35.6M | 0 |
| US GRAINS COUNCIL | Washington | — | $35.5M | 13 |
| MED-PROJECT USA | Washington | F20 Mental Health & Crisis Intervention | $35.4M | 0 |
| CLIMATE POWER | Washington | C20 Environment | $35.3M | 7 |
| Bank Policy Institute | Washington | — | $35.3M | 11 |
| NATIONAL PARKS CONSERVATION ASSOCIATION | Washington | C600 Environment | $35.1M | 12 |
| AUTISM SPEAKS INC | Washington | G128 Diseases, Disorders & Medical Disciplines | $35M | 10 |
