| URAC | Washington | S40Z Community Improvement & Capacity Building | $31.3M | 10 |
| American Battlefield Trust | Washington | A85Z Arts, Culture & Humanities | $31.2M | 9 |
| THE WASHINGTON HOUSING CONSERVANCY INC | Washington | L20 Housing & Shelter | $30.8M | 0 |
| JANE GOODALL INSTITUTE FOR WILDLIFE RESEARCH EDUCATION AND CONSERVATION | Washington | D053 Animal-Related | $30.6M | 12 |
| NATIONAL CHILDREN'S ALLIANCE INC | Washington | — | $30.5M | 8 |
| WOMEN FOR WOMEN INTERNATIONAL | Washington | Q300 International, Foreign Affairs & National Security | $30.5M | 12 |
| The Education Trust Inc | Washington | B90 Education | $30.4M | 15 |
| OPPORTUNITYWORK INC | Washington | J20 Employment | $30.4M | 14 |
| WOODROW WILSON INTERNATIONAL CENTER FOR SCHOLARS | Washington | V350 Social Science | $30.4M | 9 |
| ALLIANCE FOR AUTOMOTIVE INNOVATION INC | Washington | S41 Community Improvement & Capacity Building | $30.4M | 13 |
| IMPROVING ECONOMIES FOR STRONGER COMMUNITIES | Washington | — | $30.2M | 13 |
| WASHINGTON GAS LIGHT COMPANY MANAGEMENT VEBA | Washington | — | $30.1M | 1 |
| SOCIETY FOR NEUROSCIENCE | Washington | H030 Medical Research | $30M | 11 |
| NATIONAL AIR TRAFFIC CONTROLLERS ASSOCIATION AFL-CIO | Washington | — | $30M | 18 |
| Cotton Council International | Washington | — | $29.9M | 9 |
| RURAL COMMUNITY ASSISTANCE PARTNERSHIP INCORPORATED | Washington | — | $29.8M | 6 |
| MUNDO VERDE BILINGUAL PUBLIC CHARTER SCHOOL | Washington | B29 Education | $29.7M | 6 |
| Public Welfare Foundation Inc | Washington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $29.6M | 10 |
| American College of Cardiology | Washington | E03 Health Care | $29.5M | 28 |
| CLIMATE AND CLEAN ENERGY EQUITY FUND | Washington | C01 Environment | $29.5M | 9 |
| INTERNATIONAL CITYCOUNTY MANAGEMENT ASSOCIATION | Washington | Q023 International, Foreign Affairs & National Security | $29.5M | 8 |
| The ONE Campaign | Washington | Z99 Unknown | $29.5M | 11 |
| FEDERATION OF AMERICAN SCIENTISTS | Washington | Q41 International, Foreign Affairs & National Security | $29.4M | 7 |
| THE COUNCIL OF INSURANCE AGENTS AND BROKERS | Washington | — | $29.4M | 14 |
| BSA BUSINESS SOFTWARE ALLIANCE INC | Washington | — | $29.3M | 9 |
| SAINT COLETTA OF GREATER WASHINGTON INC | Washington | Z99 Unknown | $29.2M | 9 |
| The Partnership Project Inc | Washington | C01 Environment | $29.2M | 3 |
| ASSOCIATED BUILDERS AND CONTRACTORS INC | Washington | — | $29.1M | 16 |
| CLASP | Washington | C35 Environment | $28.9M | 9 |
| NATIONAL CAUCUS AND CENTER ON BLACK AGING INC | Washington | P81Z Human Services | $28.8M | 5 |
| PARTNERS OF THE AMERICAS INC | Washington | Q300 International, Foreign Affairs & National Security | $28.8M | 11 |
| HUMAN RIGHTS CAMPAIGN FOUNDATION | Washington | R260 Civil Rights, Social Action & Advocacy | $28.6M | 0 |
| WAY TO WIN ACTION FUND INC | Washington | R01 Civil Rights, Social Action & Advocacy | $28.5M | 0 |
| UAW RETIREES OF MACK TRUCKS HEALTH BENEFIT FUND | Washington | Y43 Mutual & Membership Benefit | $28.5M | 6 |
| HEALTHCARE DISTRIBUTION ALLIANCE | Washington | — | $28.4M | 10 |
| DEMOCRACY MATTERS | Washington | R01 Civil Rights, Social Action & Advocacy | $28.3M | 0 |
| CENTER FOR THE STUDY OF SERVICES | Washington | W050 Public & Societal Benefit | $28.3M | 12 |
| JACK & JILL OF AMERICA INC | Washington | — | $28.3M | 0 |
| NATIONAL MULTIFAMILY HOUSING COUNCIL INC | Washington | — | $28.2M | 9 |
| TWO RIVERS PUBLIC CHARTER SCHOOL INC | Washington | B24 Education | $28.2M | 6 |
| AMERICAN HUMANE ASSOCIATION | Washington | D200 Animal-Related | $28.1M | 7 |
| CORPORATION OF THE WASHINGTON LATIN SCHOOL | Washington | B29 Education | $28M | 7 |
| Council of Chief State School Officers | Washington | B20 Education | $27.9M | 12 |
| Verra | Washington | C20 Environment | $27.8M | 17 |
| RESULTS EDUCATIONAL FUND INC | Washington | B00J Education | $27.7M | 8 |
| AMERICAN HOTEL & LODGING ASSOCIATION | Washington | — | $27.5M | 8 |
| DURABLE CAPITAL FOR SUSTAINABLE OUTCOMES INC | Washington | W99 Public & Societal Benefit | $27.5M | 2 |
| GROWTH ENERGY | Washington | S41 Community Improvement & Capacity Building | $27.4M | 10 |
| IRONWORKER MANAGEMENT PROGRESSIVE ACTION COOPERATIVE TRUST | Washington | — | $27.4M | 1 |
| FINANCIAL SERVICES FORUM | Washington | S41 Community Improvement & Capacity Building | $27.4M | 8 |
