| The ONE Campaign | Washington | Z99 Unknown | $29.5M | 11 |
| BSA BUSINESS SOFTWARE ALLIANCE INC | Washington | — | $29.3M | 9 |
| INTERNATIONAL SPY MUSEUM | Washington | A50 Arts, Culture & Humanities | $29M | 7 |
| Humane Society International | Washington | D200 Animal-Related | $29M | 23 |
| CLASP | Washington | C35 Environment | $28.9M | 9 |
| NATIONAL CAUCUS AND CENTER ON BLACK | Washington | P81Z Human Services | $28.8M | 5 |
| PARTNERS OF THE AMERICAS INC | Washington | Q300 International, Foreign Affairs & National Security | $28.8M | 11 |
| Democracy Fund Voice Inc | Washington | W99 Public & Societal Benefit | $28.8M | 8 |
| THE SHAKESPEARE THEATRE | Washington | A650 Arts, Culture & Humanities | $28.7M | 8 |
| PEOPLE'S COURAGE INTERNATIONAL INC | Washington | P20 Human Services | $28.6M | 1 |
| NATIONAL CONFECTIONERS ASSOCIATION OF | Washington | — | $28.5M | 9 |
| HEALTHCARE DISTRIBUTION ALLIANCE | Washington | — | $28.4M | 10 |
| DEMOCRACY MATTERS | Washington | — | $28.3M | 0 |
| CENTER FOR THE STUDY OF SERVICES | Washington | W050 Public & Societal Benefit | $28.3M | 12 |
| JACK & JILL OF AMERICA INC | Washington | — | $28.3M | 0 |
| AMERICAN HUMANE ASSOCIATION | Washington | D200 Animal-Related | $28.1M | 7 |
| Council of Chief State School Officers | Washington | B20 Education | $27.9M | 12 |
| Verra | Washington | C20 Environment | $27.8M | 17 |
| RESULTS EDUCATIONAL FUND INC | Washington | B00J Education | $27.7M | 8 |
| INTERNATIONAL UNION OF OPERATING | Washington | J22 Employment | $27.5M | 7 |
| COUNCIL FOR ADVANCEMENT AND SUPPORT OF | Washington | — | $27.5M | 14 |
| INTERNATIONAL CITYCOUNTY MANAGEMENT | Washington | Q023 International, Foreign Affairs & National Security | $27.5M | 9 |
| DURABLE CAPITAL FOR SUSTAINABLE OUTCOMES | Washington | W99 Public & Societal Benefit | $27.5M | 2 |
| IRONWORKER MANAGEMENT PROGRESSIVE ACTION | Washington | — | $27.4M | 1 |
| FINANCIAL SERVICES FORUM | Washington | S41 Community Improvement & Capacity Building | $27.4M | 8 |
| JANE GOODALL INSTITUTE FOR WILDLIFE | Washington | D053 Animal-Related | $27.4M | 12 |
| ENDOCRINE SOCIETY | Washington | G900 Diseases, Disorders & Medical Disciplines | $27.3M | 20 |
| COUNCIL OF INSURANCE AGENTS AND BROKERS | Washington | — | $27.3M | 13 |
| RACE FORWARD | Washington | S50Z Community Improvement & Capacity Building | $27.2M | 9 |
| GROWTH ENERGY | Washington | S41 Community Improvement & Capacity Building | $27.2M | 7 |
| THIRD WAY | Washington | R99 Civil Rights, Social Action & Advocacy | $27.2M | 10 |
| American Society for Engineering | Washington | — | $27M | 5 |
| AMERICAN PUBLIC TRANSPORTATION | Washington | — | $27M | 27 |
| UNITED STATES LETTER CARRIERS | Washington | — | $27M | 7 |
| COUNCIL FOR PROFESSIONAL RECOGNITION INC | Washington | B600 Education | $26.9M | 16 |
| MARTHA'S TABLE INC | Washington | K300 Food, Agriculture & Nutrition | $26.9M | 9 |
| National Association of Counties | Washington | — | $26.8M | 11 |
| National Alliance for Public Charter | Washington | B90 Education | $26.6M | 11 |
| NATIONAL MULTIFAMILY HOUSING COUNCIL | Washington | — | $26.6M | 9 |
| PAUL PUBLIC CHARTER SCHOOL INC | Washington | B25 Education | $26.6M | 7 |
| THE LAB SCHOOL OF WASHINGTON | Washington | — | $26.5M | 7 |
| NATIONAL ALLIANCE OF STATE AND | Washington | E30Z Health Care | $26.5M | 8 |
| FORD'S THEATRE SOCIETY | Washington | A650 Arts, Culture & Humanities | $26.4M | 8 |
| GREENPEACE FUND INC | Washington | C123 Environment | $26.3M | 8 |
| American Forests | Washington | C360 Environment | $26.3M | 11 |
| AMERICAN FOREST AND PAPER ASSOCIATION | Washington | — | $26.2M | 11 |
| Better Medicare Alliance | Washington | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $26.1M | 7 |
| TWO RIVERS PUBLIC CHARTER SCHOOL INC | Washington | B24 Education | $26.1M | 5 |
| AMERICAN PUBLIC HEALTH ASSOCIATION | Washington | E70Z Health Care | $25.9M | 11 |
| ASSOCIATED BUILDERS AND CONTRACTORS INC | Washington | — | $25.9M | 13 |
