| STAR OF HOPE MISSION | Houston | L41 Housing & Shelter | $27.6M | 8 |
| Partnership With Native Americans | Addison | P20 Human Services | $27.5M | 5 |
| AUSTIN CONVENTION AND VISITORS BUREAU | Austin | — | $27.4M | 11 |
| NATIONAL DOMESTIC VIOLENCE HOTLINE | Austin | P43Z Human Services | $27.4M | 13 |
| RESPONSIVEED ARKANSAS | Lewisville | B29 Education | $27.4M | 3 |
| WESLEYAN HOMES INC | Georgetown | — | $27.3M | 6 |
| AMERICA FIRST POLICY INSTITUTE INC | Fort Worth | B05 Education | $27.3M | 10 |
| American Airlines Inc Ltd Plan Trust | Fort Worth | — | $27.3M | 1 |
| WEST TEXAS FOOD BANK | Odessa | — | $27.2M | 6 |
| ALBERT AND MARGARET ALKEK FOUNDATION | Houston | T20J Philanthropy, Voluntarism & Grantmaking Foundations | $27.1M | 6 |
| Advance Child Care Inc | Corsicana | P33 Human Services | $27.1M | 4 |
| Remember the Alamo Foundation | San Antonio | A56 Arts, Culture & Humanities | $27M | 2 |
| CAL FARLEY'S BOYS RANCH FOUNDATION | Amarillo | P110 Human Services | $27M | 2 |
| TEXAS INSTRUMENTS FOUNDATION | Dallas | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.9M | 0 |
| Los Barrios Unidos Community Clinic Inc | Dallas | — | $26.9M | 11 |
| BST SENIOR LIVING WEST INC | San Antonio | — | $26.9M | 9 |
| CENTRO DE SALUD FAMILIAR LA FE INC | El Paso | E320 Health Care | $26.8M | 10 |
| Workforce Solutions For South Texas Inc | Laredo | J22 Employment | $26.7M | 2 |
| Scott & White Continuing Care Hospital | Dallas | E20 Health Care | $26.7M | 18 |
| REGENCE HEALTH NETWORK INC | Plainview | E39Z Health Care | $26.7M | 4 |
| APV REDEVELOPMENT CORPORATION | Houston | L20 Housing & Shelter | $26.7M | 0 |
| THE LEGACY WILLOW BEND INC | Plano | P75 Human Services | $26.6M | 10 |
| The Center for Collaborative Research Inc | Houston | B11 Education | $26.5M | 8 |
| Hamill Foundation | Houston | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.5M | 5 |
| COASTAL CONSERVATION ASSOCIATION | Houston | C013 Environment | $26.5M | 12 |
| ALLEY THEATRE | Houston | A650 Arts, Culture & Humanities | $26.3M | 8 |
| HEART OF TEXAS GOODWILL INDUSTRIES INC | Waco | J32Z Employment | $26.2M | 1 |
| Bible Study Fellowship | San Antonio | X20Z Religion-Related | $26.2M | 9 |
| ARK-TEX COUNCIL OF GOVERNMENTS | Texarkana | W20C Public & Societal Benefit | $26.2M | 3 |
| Living Water International | Stafford | P20Z Human Services | $26M | 9 |
| DEEP EAST TEXAS LOCAL WORKFORCE DEVELOPMENT BOARD INC | Lufkin | J22 Employment | $26M | 2 |
| Foundation Communities Group Return | Austin | L200 Housing & Shelter | $25.9M | 10 |
| Scott & White Healthcare Foundation | Dallas | E12 Health Care | $25.9M | 19 |
| GENESYS WORKS GROUP RETURN DBA GENESYS WORKS | Houston | — | $25.9M | 12 |
| DALLAS TOURISM PUBLIC IMPROVEMENT DISTRICT CORPORATION | Dallas | S40 Community Improvement & Capacity Building | $25.9M | 1 |
| PERFORMING ARTS FORT WORTH INC | Fort Worth | A61 Arts, Culture & Humanities | $25.9M | 9 |
| HARVEY E NAJIM CHARITABLE FOUNDATION | San Antonio | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $25.9M | 1 |
| AUSTIN PETS ALIVE INC | Austin | D20 Animal-Related | $25.8M | 8 |
| CENTER FOR TRANSFORMING LIVES | Fort Worth | P270 Human Services | $25.8M | 4 |
| WOODLANDS RELIGIOUS COMMUNITY INC | The Woodlands | S22Z Community Improvement & Capacity Building | $25.8M | 8 |
| GSHS ADMINISTRATIVE SERVICES ORGANIZATIONINC | Longview | E11 Health Care | $25.8M | 4 |
| Medical Benevolence Foundation | Sugar Land | Q300 International, Foreign Affairs & National Security | $25.7M | 4 |
| YOUTH AND FAMILY ALLIANCE | Austin | P200 Human Services | $25.7M | 9 |
| SPRING BRANCH COMMUNITY HEALTH CENTER | Katy | E30 Health Care | $25.7M | 12 |
| Texas Children's Hospital Foundation | Bellaire | E112 Health Care | $25.7M | 6 |
| MISSION OF HOPE INC MISSION OF HOPE HAITI INC | Cedar Park | X21 Religion-Related | $25.7M | 4 |
| HOUSTON ASSOCIATION OF REALTORS | Houston | — | $25.6M | 28 |
| EL PASO EDUCATION INITIATIVE INC | El Paso | B20Z Education | $25.6M | 3 |
| The McCombs School of Business Foundation | Austin | B114 Education | $25.5M | 6 |
| Houston Arts Alliance | Houston | A260 Arts, Culture & Humanities | $25.5M | 7 |
