| GLOBAL IMPACT | Alexandria | Q123 International, Foreign Affairs & National Security | $145.2M | 8 |
| ASSOCIATED UNIVERSITIES INC | Vienna | U99Z Science & Technology | $143.5M | 13 |
| HAMPDEN-SYDNEY COLLEGE | Hampden Sydney | B420 Education | $141.2M | 11 |
| GREATER WASHINGTON EDUCATIONAL | Arlington | A300 Arts, Culture & Humanities | $140.6M | 13 |
| ANALYTIC SERVICES INC | Arlington | U400 Science & Technology | $138.6M | 24 |
| AMERICAN COLLEGE OF RADIOLOGY | Reston | G030 Diseases, Disorders & Medical Disciplines | $136.8M | 29 |
| SOUTHSIDE ELECTRIC COOPERATIVE INC | Crewe | — | $136.1M | 17 |
| The Methuselah Foundation | Springfield | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $129.4M | 1 |
| AMYOTROPHIC LATERAL SCLEROSIS ASSN | Arlington | G500 Diseases, Disorders & Medical Disciplines | $126.5M | 11 |
| Colonial Williamsburg Foundation | Williamsburg | A800 Arts, Culture & Humanities | $125.8M | 14 |
| AMERICAN ARMED FORCES MUTUAL AID | Fort Myer | — | $125.2M | 22 |
| 1693 Partners Fund | Williamsburg | B11 Education | $123.9M | 4 |
| TECHNOSERVE INC | Arlington | Q320 International, Foreign Affairs & National Security | $122.1M | 21 |
| MARYMOUNT UNIVERSITY | Arlington | — | $121.8M | 9 |
| THE COMMUNITY FOUNDATION INC | Richmond | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $119.3M | 10 |
| SOUTHSIDE COMMUNITY HOSPITAL INC | Farmville | E220 Health Care | $119M | 10 |
| INTERNATIONAL JUSTICE MISSION | Arlington | Q700 International, Foreign Affairs & National Security | $118.2M | 18 |
| SENTARA ENTERPRISES | Virginia Beach | E20 Health Care | $118M | 14 |
| CAPITAL IMPACT PARTNERS | Arlington | W600 Public & Societal Benefit | $115.6M | 26 |
| Consumer Technology Association | Arlington | S41 Community Improvement & Capacity Building | $115.4M | 11 |
| GOODWIN HOUSE INCORPORATED | Alexandria | P750 Human Services | $114.7M | 17 |
| CENTRAL VIRGINIA ELECTRIC | Lovingston | — | $114.6M | 17 |
| MECKLENBURG ELECTRIC COOPERATIVE | Chase City | — | $114.3M | 18 |
| GREENSPRING VILLAGE INC | Springfield | P750 Human Services | $114M | 10 |
| Marine Spill Response Corporation | Herndon | — | $110.6M | 19 |
| Shore Health Services Inc | Newport News | E220 Health Care | $108.4M | 21 |
| IN-Q-TEL INC | Tysons | Q43 International, Foreign Affairs & National Security | $108.3M | 26 |
| ROANOKE COLLEGE | Salem | B420 Education | $107.8M | 11 |
| RANDOLPH-MACON COLLEGE | Ashland | B420 Education | $107.7M | 10 |
| Native American Heritage Association | Front Royal | I40Z Crime & Legal-Related | $106.3M | 1 |
| VIRGINIA EARLY CHILDHOOD FOUNDATION | Henrico | B01 Education | $106.1M | 1 |
| FEED MORE INC | Richmond | K31 Food, Agriculture & Nutrition | $105.9M | 10 |
| GOODWILL INDUSTRIES OF THE VALLEY WORKS | Roanoke | P11 Human Services | $105.5M | 15 |
| Public Interest Registry | Reston | U054 Science & Technology | $105.1M | 21 |
| The Railroad Employees National Dental | Arlington | — | $104.9M | 1 |
| NATIONAL CENTER FOR STATE COURTS | Williamsburg | I50B Crime & Legal-Related | $104.3M | 15 |
| MIDDLE EAST BROADCASTING NETWORKS INC | Springfield | Q20 International, Foreign Affairs & National Security | $103M | 9 |
| ASHBY PONDS INC | Ashburn | P75 Human Services | $102.8M | 10 |
| UNIVERSITY OF VIRGINIA DARDEN SCHOOL | Charlottesville | B11 Education | $102.8M | 14 |
| NATIONAL STUDENT CLEARINGHOUSE | Herndon | B01 Education | $102.5M | 20 |
| COMMONWEALTH FOUNDATION FOR CANCER | Richmond | H12 Medical Research | $102.3M | 2 |
| DIDLAKE INC | Manassas | — | $101.7M | 16 |
| Operation Smile Inc | Virginia Beach | Q300 International, Foreign Affairs & National Security | $100.4M | 12 |
| Carilion Giles Community Hospital | Roanoke | E22Z Health Care | $100M | 15 |
| HALIFAX REGIONAL HOSPITAL INC | Virginia Beach | E220 Health Care | $99.3M | 14 |
| NATIONAL COUNCIL ON AGING INC | Arlington | — | $97.5M | 11 |
| Carilion Franklin Memorial Hospital | Roanoke | E220 Health Care | $97M | 15 |
| EVMS ACADEMIC PHYSICIANS AND SURGEONS | Norfolk | — | $96.4M | 23 |
| AIRPORT RESEARCH & DEVELOPMENT | Alexandria | — | $96.2M | 5 |
| UNITED NETWORK FOR ORGAN SHARING | Richmond | — | $96M | 12 |
