| Colonial Williamsburg Foundation | Williamsburg | A800 Arts, Culture & Humanities | $149.4M | 16 |
| Community Memorial Hospital | South Hill | E22 Health Care | $149M | 14 |
| GREATER WASHINGTON EDUCATIONAL TELECOMMUNICATIONS ASSOCIATION INC | Arlington | A300 Arts, Culture & Humanities | $148.4M | 15 |
| GEORGE MASON UNIVERSITY FOUNDATION INC | Fairfax | B11 Education | $145.7M | 5 |
| ASSOCIATED UNIVERSITIES INC | Vienna | U99Z Science & Technology | $143.5M | 13 |
| ANALYTIC SERVICES INC | Arlington | U400 Science & Technology | $138.6M | 24 |
| AMERICAN COLLEGE OF RADIOLOGY | Reston | G030 Diseases, Disorders & Medical Disciplines | $136.8M | 29 |
| SOUTHSIDE ELECTRIC COOPERATIVE INC | Crewe | — | $136.1M | 17 |
| OLD DOMINION LAND CONSERVANCY INC | Purcellville | B99 Education | $131.3M | 1 |
| The Methuselah Foundation | Springfield | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $129.4M | 1 |
| MARYMOUNT UNIVERSITY | Arlington | — | $127.5M | 11 |
| AMERICAN ARMED FORCES MUTUAL AID ASSOCIATION | Fort Myer | — | $127.4M | 23 |
| CENTRAL VIRGINIA ELECTRIC COOPERATIVE | Lovingston | — | $124.8M | 19 |
| TECHNOSERVE INC | Arlington | Q320 International, Foreign Affairs & National Security | $122.1M | 21 |
| INTERNATIONAL JUSTICE MISSION | Arlington | Q700 International, Foreign Affairs & National Security | $118.2M | 18 |
| SENTARA ENTERPRISES | Virginia Beach | E20 Health Care | $118M | 14 |
| SOUTHSIDE COMMUNITY HOSPITAL INC | Farmville | E220 Health Care | $116.2M | 14 |
| CAPITAL IMPACT PARTNERS | Arlington | W600 Public & Societal Benefit | $115.6M | 26 |
| Consumer Technology Association | Arlington | S41 Community Improvement & Capacity Building | $115.4M | 11 |
| Shore Health Services Inc | Newport News | E220 Health Care | $115.2M | 20 |
| GOODWIN HOUSE INCORPORATED | Alexandria | P750 Human Services | $114.7M | 17 |
| GREENSPRING VILLAGE INC | Springfield | P750 Human Services | $114M | 10 |
| MARINE SPILL RESPONSE CORPORATION | Herndon | — | $112.4M | 18 |
| ROANOKE COLLEGE | Salem | B420 Education | $111.6M | 12 |
| UNIVERSITY OF LYNCHBURG | Lynchburg | B430 Education | $108.5M | 9 |
| IN-Q-TEL INC | Tysons | Q43 International, Foreign Affairs & National Security | $108.3M | 26 |
| ONE NATION | Haymarket | W24 Public & Societal Benefit | $106.8M | 8 |
| DIDLAKE INC | Manassas | — | $106.5M | 16 |
| HAMPDEN-SYDNEY COLLEGE | Hampden Sydney | B420 Education | $106.3M | 11 |
| Native American Heritage Association | Front Royal | I40Z Crime & Legal-Related | $106.3M | 1 |
| VIRGINIA EARLY CHILDHOOD FOUNDATION | Henrico | B01 Education | $106.1M | 1 |
| BEDFORD FALLS FOUNDATION | McLean | T22Z Philanthropy, Voluntarism & Grantmaking Foundations | $105.2M | 0 |
| Public Interest Registry | Reston | U054 Science & Technology | $105.1M | 21 |
| NATIONAL CENTER FOR STATE COURTS | Williamsburg | I50B Crime & Legal-Related | $104.3M | 15 |
| GOODWILL INDUSTRIES OF THE VALLEY WORKS | Roanoke | P11 Human Services | $104.2M | 15 |
| UVA PRINCE WILLIAM HEALTH SYSTEM | Manassas | — | $103.6M | 12 |
| MIDDLE EAST BROADCASTING NETWORKS INC | Springfield | Q20 International, Foreign Affairs & National Security | $103M | 9 |
| ASHBY PONDS INC | Ashburn | P75 Human Services | $102.8M | 10 |
| CAPITAL ONE FOUNDATION INC | McLean | B82Z Education | $102.8M | 0 |
| UNIVERSITY OF VIRGINIA DARDEN SCHOOL FOUNDATION | Charlottesville | B11 Education | $102.8M | 14 |
| NATIONAL STUDENT CLEARINGHOUSE | Herndon | B01 Education | $102.5M | 20 |
| RANDOLPH-MACON COLLEGE | Ashland | B420 Education | $101.9M | 11 |
| THE COMMUNITY FOUNDATION INC | Richmond | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $101.7M | 10 |
| PACKAGING MACHINERY MANUFACTURERS INSTITUTE | Herndon | — | $101.4M | 8 |
| MILITARY AVIATION MUSEUM FOUNDATION | Virginia Beach | A11 Arts, Culture & Humanities | $101M | 0 |
| Operation Smile Inc | Virginia Beach | Q300 International, Foreign Affairs & National Security | $100.4M | 12 |
| Carilion Giles Community Hospital | Roanoke | E22Z Health Care | $100M | 15 |
| DUPONT COMMUNITY CREDIT UNION | Waynesboro | — | $98.2M | 12 |
| HALIFAX REGIONAL HOSPITAL INC | Virginia Beach | E220 Health Care | $98.2M | 13 |
| NATIONAL COUNCIL ON AGING INC | Arlington | — | $97.5M | 11 |
