| Carilion Franklin Memorial Hospital | Roanoke | E220 Health Care | $97M | 15 |
| EVMS ACADEMIC PHYSICIANS AND SURGEONS HEALTH SERVICES FOUNDATION | Norfolk | — | $96.4M | 23 |
| FEED MORE INC | Richmond | K31 Food, Agriculture & Nutrition | $96.2M | 10 |
| AIRPORT RESEARCH & DEVELOPMENT FOUNDATION | Alexandria | — | $96.2M | 5 |
| UNITED NETWORK FOR ORGAN SHARING | Richmond | — | $96M | 12 |
| TEAMSTERS JOINT COUNCIL NO 83 OF VIRGINIA HEALTH & WELFARE FUND | Richmond | — | $95.4M | 4 |
| The Railroad Employees National Dental Plan | Arlington | — | $94.7M | 2 |
| ETHIOPIAN COMMUNITY DEV COUNCIL INC | Arlington | P840 Human Services | $92.4M | 2 |
| WESTMINSTER CANTERBURY CORPORATION | Richmond | P750 Human Services | $92.4M | 14 |
| MPB INC | Virginia Beach | S47 Community Improvement & Capacity Building | $90.6M | 5 |
| Community Housing Partners Corporation | Christiansburg | — | $90.3M | 11 |
| BRIDGEWATER COLLEGE | Bridgewater | B420 Education | $89.6M | 11 |
| A & N ELECTRIC COOPERATIVE | Tasley | — | $88.3M | 18 |
| UNIVERSITY OF VIRGINIA COMMUNITY CREDIT UNION | Charlottesville | — | $87.8M | 18 |
| REHAB JV LLC | Richmond | E50 Health Care | $87.2M | 10 |
| IMAGINE SCHOOLS NON-PROFIT INC | Vienna | B200 Education | $86.2M | 9 |
| INTERNATIONAL INFORMATION SYSTEM SECURITY CERTIFICATION CONSORTIUM INC | Alexandria | — | $86.1M | 13 |
| CENTRAL VIRGINIA HEALTH SERVICES INC | New Canton | E32Z Health Care | $86M | 5 |
| Richfield Living | Salem | E91Z Health Care | $85.9M | 4 |
| VIRGINIA WESLEYAN UNIVERSITY | Virginia Beach | — | $85.9M | 13 |
| Augusta Medical Group | Fishersville | E60 Health Care | $84.7M | 14 |
| VIRGINIA UNITED METHODIST HOMES INC | Glen Allen | P750 Human Services | $83.9M | 14 |
| National Automobile Dealers Association | Tysons | — | $83.8M | 13 |
| MARCH OF DIMES INC | Arlington | G200 Diseases, Disorders & Medical Disciplines | $83.6M | 12 |
| TROUT UNLIMITED INC | Arlington | D330 Animal-Related | $83.5M | 9 |
| Carilion Rockbridge Community Hospital | Roanoke | E220 Health Care | $83.1M | 16 |
| CONFEDERATION OF INDIAN INDUSTRY | Arlington | — | $82.9M | 1 |
| YOUTH FOR TOMORROW FOUNDATION | Bristow | O11 Youth Development | $82.7M | 1 |
| WOODBERRY FOREST SCHOOL | Woodberry Forest | B250 Education | $82.6M | 10 |
| EMORY & HENRY UNIVERSITY | Emory | B420 Education | $82.5M | 6 |
| THE MOUNT VERNON LADIES' ASSOCIATION OF THE UNION | Mount Vernon | A800 Arts, Culture & Humanities | $82.2M | 13 |
| NATIONAL EXPERIENCED WORKFORCE SOLUTIONS | Arlington | J33 Employment | $81.6M | 8 |
| RED GATES FOUNDATION | Richmond | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $79.3M | 0 |
| Operation Blessing International Relief and Development Corporation | Virginia Beach | Q330 International, Foreign Affairs & National Security | $78.2M | 12 |
| INTERNATIONAL FOUNDATION FOR ELECTORAL SYSTEMS | Arlington | Q300 International, Foreign Affairs & National Security | $77.5M | 10 |
| PEOPLE FOR THE ETHICAL TREATMENT OF ANIMALS INC | Norfolk | D010 Animal-Related | $77.2M | 8 |
| BLUE RIDGE AREA FOOD BANK INC | Verona | — | $76.5M | 6 |
| Fringe Benefit Plan for Service Contract Act Employees of Maximus Serices LLC | McLean | Y43 Mutual & Membership Benefit | $75.9M | 0 |
| ASSOCIATION OF STATE AND TERRITORIAL HEALTH OFFICIALS | Arlington | E010 Health Care | $75M | 13 |
| GRAFTON SCHOOL INCORPORATED | Winchester | B280 Education | $74M | 13 |
| US CIVILIAN RESEARCH & DEVELOPMENT FOUNDATION | Arlington | U120 Science & Technology | $73.6M | 23 |
| UNITED WAY WORLDWIDE | Alexandria | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $72.4M | 16 |
| WESTMINSTER CANTERBURY ON CHESAPEAKE BAY | Virginia Beach | P750 Human Services | $71.6M | 13 |
| The Potomac School | McLean | B200 Education | $71.5M | 7 |
| KISSITO HEALTHCARE INC AND AFFILIATES | Roanoke | — | $71.4M | 8 |
| Human Resources Research Organization | Alexandria | V230 Social Science | $70.4M | 21 |
| HOLLINS UNIVERSITY CORPORATION | Roanoke | B430 Education | $69.9M | 9 |
| ENTREPRENEURS ORGANIZATION | Alexandria | B036 Education | $69.6M | 11 |
| AMYOTROPHIC LATERAL SCLEROSIS ASSN | Arlington | G500 Diseases, Disorders & Medical Disciplines | $68.6M | 13 |
| PRISON FELLOWSHIP MINISTRIES | Lansdowne | I430 Crime & Legal-Related | $68.4M | 20 |
