| SENTARA MEDICAL GROUP | Virginia Beach | E63Z Health Care | $381.4M | 41 |
| THE CONSERVATION FUND A NONPROFIT | Arlington | C300 Environment | $373.2M | 14 |
| LEIDOS HEALTH AND WELFARE BENEFITS TRUST | Reston | — | $365.2M | 1 |
| Carilion New River Valley Medical Center | Roanoke | E220 Health Care | $358.1M | 13 |
| UNIVERSITY OF VIRGINIA INVESTMENT | Charlottesville | B11 Education | $355.3M | 15 |
| INSTITUTE FOR DEFENSE ANALYSES | Alexandria | U050 Science & Technology | $350.3M | 25 |
| GROUP HEALTH PROGRAM CO NATIONAL | Arlington | E81Z Health Care | $342.8M | 2 |
| VIRGINIA CREDIT UNION INC | Richmond | — | $341.8M | 17 |
| AIR LINE PILOTS ASSOCIATION INTERNATIONAL | McLean | — | $340.3M | 42 |
| UVA PRINCE WILLIAM MEDICAL CENTER | Manassas | E220 Health Care | $322.3M | 12 |
| NAVY MUTUAL AID ASSOCIATION | Arlington | W30Z Public & Societal Benefit | $311.8M | 12 |
| POTOMAC HOSPITAL CORPORATION | Virginia Beach | E220 Health Care | $311.1M | 17 |
| THE CHRISTIAN BROADCASTING NETWORK INC | Virginia Beach | X800 Religion-Related | $300.1M | 21 |
| SHENANDOAH VALLEY ELECTRIC | Rockingham | — | $293.1M | 17 |
| EDWARD VIA VIRGINIA COLLEGE OF | Blacksburg | B50 Education | $289.9M | 21 |
| WASHINGTON AND LEE UNIVERSITY | Lexington | B420 Education | $276.6M | 13 |
| PATIENT ADVOCATE FOUNDATION | Hampton | B90 Education | $243.5M | 8 |
| CONSERVATION INTERNATIONAL FOUNDATION | Arlington | C30Z Environment | $235.1M | 12 |
| Compass Rose Benefits Group | Reston | — | $230.8M | 21 |
| VIRGINIA TECH FOUNDATION INC | Blacksburg | B110 Education | $222.3M | 9 |
| THE ARHANT SOCIAL FOUNDATION INC | Clifton | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $221M | 0 |
| National Rural Electric Cooperative | Arlington | — | $213.2M | 59 |
| Childfund International USA | Richmond | Q330 International, Foreign Affairs & National Security | $210.2M | 7 |
| Society for Human Resource Management | Alexandria | S41 Community Improvement & Capacity Building | $198.7M | 26 |
| AMERICAN TYPE CULTURE COLLECTION | Manassas | U500 Science & Technology | $198M | 23 |
| HAMPTON UNIVERSITY | Hampton | B430 Education | $197.4M | 8 |
| VALLEY HEALTH SYSTEM | Winchester | E21 Health Care | $189.9M | 53 |
| RIVERSIDE RESEARCH INSTITUTE | Fairfax | H050 Medical Research | $189.3M | 25 |
| AMERICANS FOR PROSPERITY | Arlington | S31 Community Improvement & Capacity Building | $186.4M | 11 |
| NATIONAL STABILIZATION AGREEMENT OF THE | Falls Church | — | $182M | 7 |
| NATIONAL RIFLE ASSOCIATION OF AMERICA | Fairfax | R60 Civil Rights, Social Action & Advocacy | $175.7M | 18 |
| AMERICAN SOCIETY OF CLINICAL ONCOLOGY | Alexandria | G9XC Diseases, Disorders & Medical Disciplines | $174.3M | 17 |
| SOURCEAMERICA | Vienna | J300 Employment | $173.6M | 9 |
| THE SEMINAR NETWORK INC | Arlington | B99 Education | $172.6M | 5 |
| MARY WASHINGTON HEALTHCARE | Fredericksburg | E210 Health Care | $171.8M | 31 |
| MANAGEMENT SCIENCES FOR HEALTH INC | Arlington | Q300 International, Foreign Affairs & National Security | $168.8M | 27 |
| REGENT UNIVERSITY | Virginia Beach | — | $168.2M | 15 |
| UNITED SERVICE ORGANIZATIONS INC | Arlington | W30Z Public & Societal Benefit | $168M | 13 |
| ISLAMIC RELIEF USA | Alexandria | Q123 International, Foreign Affairs & National Security | $165.2M | 10 |
| ARMED FORCES BENEFIT ASSOCIATION | Alexandria | — | $162.9M | 23 |
| UVA CULPEPER MEDICAL CENTER | Culpeper | E220 Health Care | $162.1M | 14 |
| Donors Trust Inc | Alexandria | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $158.3M | 9 |
| NATIONAL WILDLIFE FEDERATION | Reston | D300 Animal-Related | $156.4M | 18 |
| BAYPORT CREDIT UNION | Newport News | — | $152.8M | 10 |
| SERVICESOURCE INC | Oakton | J300 Employment | $152.4M | 15 |
| American Diabetes Association | Arlington | G800 Diseases, Disorders & Medical Disciplines | $152.2M | 10 |
| CNA CORPORATION | Arlington | — | $151.7M | 27 |
| SHENANDOAH UNIVERSITY | Winchester | B430 Education | $149.2M | 15 |
| Community Memorial Hospital | South Hill | E22 Health Care | $149M | 14 |
| GEORGE MASON UNIVERSITY FOUNDATION INC | Fairfax | B11 Education | $145.7M | 5 |
