| WISCONSIN EVANGELICAL LUTHERAN SYNOD | Wauwatosa | — | $68.1M | 0 |
| THE LAKES COMMUNITY HEALTH CENTER INC | Iron River | — | $67.9M | 9 |
| CHILDREN'S SERVICE SOCIETY OF WISCONSIN | Milwaukee | — | $66.9M | 14 |
| SIXTEENTH STREET COMMUNITY HEALTH CENTERS INC | Milwaukee | — | $66.8M | 7 |
| ISABEL BADER 2021 CHARITABLE TRUST | Milwaukee | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $66.2M | 1 |
| NATIONAL CONFERENCE OF BAR EXAMINERS | Madison | I030 Crime & Legal-Related | $65.1M | 11 |
| Rock Energy Cooperative | Janesville | — | $64.9M | 16 |
| VITERBO UNIVERSITY | La Crosse | B420 Education | $64.2M | 8 |
| UNITED WAY OF GREATER MILWAUKEE & WAUKESHA COUNTY INC | Milwaukee | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $64.2M | 7 |
| ALFRED AND ISABEL BADER 2014 CHARITABLE TRUST | Milwaukee | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $63.7M | 0 |
| BETTERLIFE | Madison | — | $63.5M | 28 |
| EDGEWOOD COLLEGE INC | Madison | B430 Education | $62.9M | 13 |
| The Lynde and Harry Bradley Foundation Inc | Milwaukee | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $61M | 12 |
| COMMUNITY FOUNDATION FOR THE FOX VALLEY REGION INC | Appleton | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $60.6M | 6 |
| LADD MEMORIAL HOSPITAL INC | Osceola | E220 Health Care | $59.3M | 12 |
| RIPON COLLEGE | Ripon | B420 Education | $56.7M | 8 |
| WESTBY CO-OP CREDIT UNION | Westby | — | $56.6M | 13 |
| BADER PHILANTHROPIES INC | Milwaukee | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $55.9M | 1 |
| GRANT REGIONAL HEALTH CENTER INC | Lancaster | E320 Health Care | $54.8M | 15 |
| WISCONSIN LUTHERAN COLLEGE INC | Milwaukee | — | $54.2M | 7 |
| EXPERIMENTAL AIRCRAFT ASSOCIATION INC | Oshkosh | U030 Science & Technology | $53.9M | 10 |
| UNITED COMMUNITY CENTER INC | Milwaukee | P840 Human Services | $53.3M | 8 |
| WISCONSIN SHEET METAL WORKERS HEALTH AND BENEFIT FUND | Waukesha | — | $53.1M | 7 |
| FAMILY HEALTH CENTER OF MARSHFIELD INC | Marshfield | E30Z Health Care | $52.4M | 9 |
| SECOND HARVEST FOODBANK OF SOUTHERN WI | Madison | K31Z Food, Agriculture & Nutrition | $51.9M | 4 |
| QUARTZ HEALTH PLAN MN CORPORATION | Fitchburg | E80 Health Care | $51.6M | 6 |
| ASSOCIATED BANC-CORP GROUP HEALTH | Green Bay | — | $51.2M | 0 |
| THEDACARE MEDICAL CENTER - SHAWANO INC | Appleton | E220 Health Care | $50.5M | 0 |
| 170 FREEDOM MILWAUKEE 2024 HOST COMMITTEE INC | Milwaukee | S30 Community Improvement & Capacity Building | $50.1M | 2 |
| WISCONSIN COMMUNITY SERVICES INC | Milwaukee | I40Z Crime & Legal-Related | $49.9M | 7 |
| NORTH CENTRAL STATES REGIONAL COUNCIL OF CARPENTERS | St Paul | S41 Community Improvement & Capacity Building | $49.7M | 18 |
| ST AUGUSTINE PREPARATORY ACADEMY INC | Milwaukee | B19 Education | $49.6M | 8 |
| LAKELAND UNIVERSITY | Plymouth | B420 Education | $49.4M | 14 |
| SUPERIOR CHOICE CREDIT UNION | Superior | — | $48.6M | 21 |
| THE UWM FOUNDATION INC | Milwaukee | B430 Education | $48.6M | 6 |
| OAKDALE ELECTRIC COOPERATIVE | Oakdale | — | $48.3M | 15 |
| WISCONSIN PIPE TRADES HEALTH FUND | Milwaukee | — | $47.6M | 4 |
| GREATER WISCONSIN AGENCY ON AGING RESOURCES INC | Madison | — | $47.2M | 1 |
| RIVERLAND ENERGY COOPERATIVE | Arcadia | — | $46.5M | 15 |
| BLACKHAWK COMMUNITY CREDIT UNION | Janesville | — | $46.3M | 15 |
| ORC Industries Inc | La Crosse | J330 Employment | $46.3M | 4 |
| Historic Haymarket Milwaukee Inc | Milwaukee | A50 Arts, Culture & Humanities | $46.1M | 0 |
| ACCESS COMMUNITY HEALTH CENTERS INC | Madison | — | $45.6M | 20 |
| HORIZON HOME CARE & HOSPICE INC | Milwaukee | E99Z Health Care | $45.5M | 10 |
| ST JOSEPH'S HEALTH SERVICES INC | Hillsboro | E22 Health Care | $44.6M | 16 |
| BARRON ELECTRIC COOPERATIVE | Barron | — | $43.6M | 16 |
| BENEVOLENT CORPORATION CEDAR COMMUNITY | West Bend | P750 Human Services | $43.5M | 8 |
| ALVERNO COLLEGE | Milwaukee | B420 Education | $42.5M | 12 |
| GOODWILL INDUSTRIES OF SOUTH CENTRAL WISCONSIN INC | Madison | J32Z Employment | $42.5M | 6 |
| Milwaukee Center For Independence Inc | Milwaukee | P8XZ Human Services | $42.4M | 9 |
