| THE UNIVERSITY OF FLORIDA FOUNDATION INC | Gainesville | B110 Education | $269.1M | 17 |
| Florida Hospital Healthcare Partners Inc | Ormond Beach | — | $254.5M | 15 |
| LADIES PROFESSIONAL GOLF ASSOCIATION | Daytona Beach | — | $252.6M | 22 |
| STETSON UNIVERSITY INC | DeLand | B430 Education | $250.4M | 21 |
| FLORIDA HOSPITAL ZEPHYRHILLS INC | Zephyrhills | E220 Health Care | $246.7M | 33 |
| The Villages Tri-County Medical Center Inc | The Villages | E220 Health Care | $239.6M | 33 |
| Flagler Hospital Inc | St Augustine | E220 Health Care | $239M | 36 |
| CITRUS HEALTH NETWORK INC | Hialeah | — | $230.6M | 16 |
| CAMELOT COMMUNITY CARE INC | Clearwater | P200 Human Services | $228.3M | 10 |
| ROLLINS COLLEGE | Winter Park | B420 Education | $226.8M | 17 |
| ATP Tour Inc | Ponte Vedra Beach | — | $225.3M | 26 |
| FLORIDA INSTITUTE OF TECHNOLOGY INC | Melbourne | B430 Education | $222.5M | 14 |
| FAMILY SUPPORT SERVICES OF NORTH FLORIDA INC | Jacksonville | P80 Human Services | $222.3M | 11 |
| FARM SHARE INC | Homestead | K30Z Food, Agriculture & Nutrition | $220.8M | 3 |
| BayCare Home Care Inc | Largo | E92 Health Care | $218.8M | 13 |
| Baptist Medical Center of the Beaches Inc | Jacksonville | E220 Health Care | $218.8M | 12 |
| Employee Welfare Benefit Plans Trust of FPL Group Inc & Its Affiliates | Juno Beach | — | $216M | 1 |
| SOLARIS FOUNDATION INC | Windermere | E91 Health Care | $215.6M | 13 |
| Southeast Volusia Healthcare Corporation | New Smyrna Beach | N22 Recreation & Sports | $214.9M | 28 |
| CAPE CANAVERAL HOSPITAL INC | Rockledge | E220 Health Care | $214.5M | 11 |
| FAIRWINDS CREDIT UNION | Orlando | — | $214.5M | 13 |
| FEEDING AMERICA TAMPA BAY INC | Tampa | K31Z Food, Agriculture & Nutrition | $212.9M | 9 |
| Baptist Outpatient Services Inc | Coral Gables | E30 Health Care | $212.3M | 10 |
| VIERA HOSPITAL INC | Rockledge | E20 Health Care | $205.8M | 12 |
| COMMUNITY FOUNDATION OF TAMPA BAY INC | Tampa | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $203.6M | 7 |
| South Florida Baptist Hospital Inc | Plant City | E220 Health Care | $202.7M | 12 |
| Jacksonville University | Jacksonville | B430 Education | $197.9M | 9 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA | Jonesville | — | $197.8M | 18 |
| GOODWILL INDUSTRIES OF SOUTH FLORIDA INC | Miami | J32Z Employment | $196.1M | 9 |
| FLORIDA SOUTHERN COLLEGE | Lakeland | B420 Education | $195.7M | 11 |
| ECKERD YOUTH ALTERNATIVES INC | Clearwater | P30 Human Services | $194.7M | 13 |
| Barry University Inc | Miami Shores | B430 Education | $193.9M | 16 |
| GENESIS REHABILITATION HOSPITAL INC | Jacksonville | E240 Health Care | $192.8M | 15 |
| UNIVERSITY ATHLETIC ASSOCIATION INC | Gainesville | N400 Recreation & Sports | $191.9M | 15 |
| EARLY LEARNING COALITION OF PALM BEACH COUNTY INC | Boynton Beach | B012 Education | $190.6M | 5 |
| Tarpon Springs Hospital Foundation Inc | Tarpon Springs | E220 Health Care | $190.2M | 27 |
| FEEDING SOUTH FLORIDA INC | Pembroke Park | K31Z Food, Agriculture & Nutrition | $187.9M | 5 |
| EARLY LEARNING COALITION OF BROWARD COUNTY INC | Fort Lauderdale | P12 Human Services | $187.8M | 5 |
| PINECREST ACADEMY INC | Miami | B29 Education | $184.9M | 21 |
| THE SPURLINO FOUNDATION | Odessa | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $179.3M | 2 |
| PENAIR CREDIT UNION | Pensacola | — | $174.3M | 23 |
| ADDITION FINANCIAL CREDIT UNION | Lake Mary | W61 Public & Societal Benefit | $172.6M | 17 |
| SECOND HARVEST FOOD BANK OF CENTRAL FLORIDA INC | Orlando | — | $172.1M | 10 |
| COMMUNITY FIRST CREDIT UNION OF FLORIDA | Jacksonville | — | $172M | 12 |
| THE LEON AND TOBY COOPERMAN FAMILY FOUNDATION | Boca Raton | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $170.7M | 0 |
| Florida Credit Union | Gainesville | — | $169.4M | 14 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA | Dunedin | — | $167.7M | 19 |
| LIFELINK FOUNDATION INC | Tampa | E650 Health Care | $167.7M | 31 |
| AdventHealth Primary Care Network Inc | Altamonte Springs | — | $166.4M | 11 |
| PRESBYTERIAN RETIREMENT COMMUNITIES INC | Orlando | P750 Human Services | $163.9M | 8 |
