| Bethesda Hospital Inc | Boynton Beach | E220 Health Care | $519.5M | 13 |
| SOMERSET ACADEMY INC | Miami | B20Z Education | $498.4M | 49 |
| SUMTER ELECTRIC COOPERATIVE INC | Sumterville | — | $483M | 24 |
| FLORIDA INSURANCE GUARANTY | Tallahassee | — | $478.4M | 5 |
| HEALTH FIRST HEALTH PLANS INC | Rockledge | E21 Health Care | $474.1M | 8 |
| Jupiter Medical Center Inc | Jupiter | E220 Health Care | $464.3M | 9 |
| UMA EDUCATION INC | Tampa | B30 Education | $457.3M | 22 |
| OHI West Inc | St Petersburg | E22 Health Care | $456.8M | 12 |
| The University of Tampa Incorporated | Tampa | B430 Education | $450.9M | 10 |
| ONEBLOOD INC | Orlando | E610 Health Care | $450.7M | 12 |
| Orlando Health Central Inc | Ocoee | E22 Health Care | $448.2M | 13 |
| Winter Haven Hospital Inc | Winter Haven | E220 Health Care | $440.8M | 12 |
| SPACE COAST CREDIT UNION | Melbourne | — | $440.8M | 28 |
| CAN COMMUNITY HEALTH INC | Lakewood Ranch | G81Z Diseases, Disorders & Medical Disciplines | $440.1M | 8 |
| South Lake Hospital Inc | Clermont | E220 Health Care | $437.9M | 14 |
| FLORIDA HOSPITAL WATERMAN INC | Tavares | E220 Health Care | $427.1M | 31 |
| UNIVERSITY OF FLORIDA JACKSONVILLE | Jacksonville | E300 Health Care | $425.4M | 31 |
| Pasco-Pinellas Hillsborough Community | Wesley Chapel | E21 Health Care | $418.6M | 23 |
| FOOD FOR THE POOR INC | Coconut Creek | Q330 International, Foreign Affairs & National Security | $412.1M | 13 |
| MIDFLORIDA CREDIT UNION | Lakeland | — | $407.8M | 19 |
| RENAISSANCE CHARTER SCHOOL INC | Fort Lauderdale | B020 Education | $389.7M | 0 |
| HEALTH FIRST SHARED SERVICES INC | Rockledge | E21 Health Care | $388.9M | 26 |
| WOUNDED WARRIOR PROJECT INC | Jacksonville | P60 Human Services | $385.2M | 15 |
| LUTHERAN SERVICES FLORIDA INC | Tampa | P200 Human Services | $376.9M | 14 |
| UNIVERSITY MEDICAL SERVICE | Tampa | B99Z Education | $370.1M | 21 |
| DECHOMAI FOUNDATION INC | Jacksonville | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $363.5M | 0 |
| THE ASSISTANCE FUND INC | Orlando | E99 Health Care | $341.7M | 12 |
| FLAGLER HOSPITAL INC | St Augustine | E220 Health Care | $333.7M | 27 |
| HALIFAX STAFFING INC | Daytona Beach | S50Z Community Improvement & Capacity Building | $332.1M | 7 |
| Florida Hospital Physician Group Inc | Tampa | — | $326.6M | 15 |
| Doctors Hospital Inc | Coral Gables | E22 Health Care | $325.4M | 14 |
| Miami Children's Health System Inc | Miami | E32 Health Care | $324.1M | 10 |
| MATER ACADEMY INC | Miami | B240 Education | $314.4M | 5 |
| MEMORIAL HOSPITAL FLAGLER INC | Palm Coast | E22Z Health Care | $313.9M | 29 |
| H LEE MOFFITT CANCER CENTER AND | Tampa | G053 Diseases, Disorders & Medical Disciplines | $310.1M | 31 |
| BETTY WOLD JOHNSON FOUNDATION | Palm Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $304.4M | 0 |
| Baptist Health System Inc | Jacksonville | E210 Health Care | $299.4M | 24 |
| CENTRAL FLORIDA BEHAVIORAL | Tampa | P30 Human Services | $298.6M | 5 |
| EARLY LEARNING COALITION OF MIAMI | Coral Gables | B21 Education | $293.6M | 11 |
| THE VILLAGES CHARTER SCHOOL INC | The Villages | B20 Education | $292.9M | 2 |
| THE FLORIDA BAR FOUNDATION INC | Maitland | I120 Crime & Legal-Related | $285.3M | 4 |
| ALFRED I DUPONT CHARITABLE TRUST | Jacksonville | E12 Health Care | $284.6M | 15 |
| AdventHealth Polk North Inc | Davenport | — | $282.6M | 23 |
| LEESAR INC | Fort Myers | — | $280.8M | 11 |
| SOUTHWEST VOLUSIA HEALTHCARE CORPORATION | Orange City | E220 Health Care | $277.8M | 27 |
| EARLY LEARNING COALITION OF PALM | Boynton Beach | B012 Education | $276.6M | 5 |
| THE LOWELL S & BETTY L DUNN FAMILY | Hialeah | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $266.9M | 0 |
| Leesburg Regional Medical Center Inc | Leesburg | E220 Health Care | $263.1M | 32 |
| ATP Tour Inc | Ponte Vedra Beach | — | $259.5M | 23 |
| Florida Hospital Healthcare Partners | Ormond Beach | — | $254.5M | 15 |
