| American Kennel Club Inc | New York | D60 Animal-Related | $99.6M | 25 |
| SMILE TRAIN INC | New York | — | $99.6M | 11 |
| CHARLES B WANG COMMUNITY HEALTH CENTER INC | New York | E30Z Health Care | $99.6M | 10 |
| INDEPENDENT LIVING FOR SENIORS INC | Rochester | X00Z Religion-Related | $99.4M | 12 |
| TOURO UNIVERSITY | New York | — | $99.3M | 18 |
| PLUMBERS LOCAL UNION NO 1 WELFARE FUND | Long Island City | Y43 Mutual & Membership Benefit | $99.1M | 9 |
| NEW YORK PSYCHOTHERAPY AND COUNSELING CENTER | Jamaica | F320 Mental Health & Crisis Intervention | $98.8M | 16 |
| Garnet Health Medical Center - Catskills | Harris | E220 Health Care | $98.5M | 13 |
| Westchester Health Medical PC | Westbury | E20 Health Care | $98.4M | 12 |
| HARLEM CHILDREN'S ZONE INC | New York | P300 Human Services | $98.2M | 15 |
| NYSARC INC ULSTER GREENE PUTNAM COUNTY CHAPTERS | Kingston | P820 Human Services | $98.2M | 9 |
| RIVERDALE COUNTRY SCHOOL INC | Bronx | B200 Education | $98M | 15 |
| VISITING NURSE SERVICE OF NEW YORK HOSPICE CARE | New York | P74 Human Services | $97.8M | 10 |
| RESOURCE CENTER FOR INDEPENDENT LIVING INC | Utica | P8XZ Human Services | $97.8M | 5 |
| UNITE HERE | New York | — | $97.5M | 21 |
| IUOE LOCAL 30 BENEFITS FUND | Whitestone | — | $97.3M | 11 |
| NORTH SHORE COMMUNITY SERVICES INC C/O NORTHWELL HEALTH INC | Westbury | — | $97.3M | 11 |
| Central Fund of Israel | Cedarhurst | Q123 International, Foreign Affairs & National Security | $96.9M | 1 |
| SAFE HORIZON INC | New York | P620 Human Services | $96.7M | 13 |
| ELECTRICAL EMPLOYERS SELF INSURANCE SAFETY PLAN | Flushing | — | $96.4M | 0 |
| FUND FOR PUBLIC HEALTH IN NEW YORK INC | New York | G80 Diseases, Disorders & Medical Disciplines | $96.4M | 6 |
| AMERICAN NATIONAL STANDARDS INSTITUTE | New York | W99 Public & Societal Benefit | $96.3M | 14 |
| AURELIA OSBORN FOX MEMORIAL HOSPITAL | Oneonta | E220 Health Care | $96.3M | 8 |
| THE KLINGENSTEIN-MARTELL FOUNDATION FKA THE SARAH D KLINGENSTEIN FOUNDATION | New York | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $96.2M | 0 |
| MJHS HOSPICE AND PALLIATIVE CARE INC | New York | P74 Human Services | $95.7M | 9 |
| DAVID C BUTTERFIELD AND ALFONSO D RUBIO MEMORIAL FOUNDATION | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $95.6M | 0 |
| The Conference Board Inc | New York | S400 Community Improvement & Capacity Building | $95.5M | 16 |
| CATHOLIC GUARDIAN SERVICES | New York | — | $95.3M | 9 |
| ASPIRE OF WESTERN NEW YORK INC | Getzville | L80 Housing & Shelter | $95.2M | 9 |
| UTICA UNIVERSITY | Utica | B420 Education | $94.5M | 8 |
| JEWISH CHILD CARE ASSOCIATION OF NEW YORK | Brooklyn | P200 Human Services | $94.2M | 8 |
| THE HABE FOUNDATION | New York | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $94.1M | 0 |
| COVENANT HOUSE | New York | P300 Human Services | $93.9M | 10 |
| PHELPS MEDICAL SERVICES PC | Sleepy Hollow | E30 Health Care | $93.8M | 6 |
| PAVERS & ROADBUILDERS DC WELFARE FUND | Whitestone | — | $93.7M | 9 |
| THE CHILD CENTER OF NY INC | Forest Hills | — | $93.7M | 7 |
| NATIONAL SEPTEMBER 11 MEMORIAL AND MUSEUM AT THE WORLD TRADE CENTER | New York | A84 Arts, Culture & Humanities | $93.3M | 13 |
| LIVEONNY INC | Long Island City | E65 Health Care | $93.3M | 15 |
| DAEMEN UNIVERSITY | Amherst | B420 Education | $93.1M | 15 |
| NICHOLAS H NOYES MEMORIAL HOSPITAL | Dansville | E220 Health Care | $93.1M | 12 |
| RPCI ONCOLOGY PC | Buffalo | E21 Health Care | $92.9M | 11 |
| NEW YORK GENOME CENTER INC | New York | E22 Health Care | $92.9M | 14 |
| COLUMBIA GRAMMAR AND PREPARATORY SCHOOL | New York | B200 Education | $92.7M | 13 |
| MEDICAL DEBT RESOLUTION INC | Long Island City | P50 Human Services | $92.6M | 10 |
| SPRINGBROOK NY INC | Oneonta | P73Z Human Services | $92.4M | 3 |
| CENTER FOR URBAN COMMUNITY SERVICES | New York | S200 Community Improvement & Capacity Building | $92.2M | 9 |
| THE ANIMAL MEDICAL CENTER | New York | D400 Animal-Related | $91.9M | 10 |
| REFUAH HEALTH CENTER INC | Spring Valley | E320 Health Care | $91.7M | 7 |
| COMMUNITY HEALTHCARE NETWORK | New York | E320 Health Care | $90.8M | 13 |
| BIG EAST CONFERENCE INC | New York | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $90.7M | 8 |
