| INTERNATIONAL ASSOCIATION OF BETTER | Arlington | P20 Human Services | $18.7M | 8 |
| VERTICAL AVIATION INTERNATIONAL INC | Alexandria | — | $18.7M | 8 |
| CROSSROADS COMMUNITY SERVICES BOARD | Farmville | F32Z Mental Health & Crisis Intervention | $18.7M | 7 |
| The Joshua Fund | McLean | X99 Religion-Related | $18.6M | 6 |
| THE BLUE RIDGE SCHOOL INC | St George | B20I Education | $18.6M | 5 |
| NATIONAL SCIENCE TEACHING ASSOCIATION | Richmond | B030 Education | $18.6M | 15 |
| United Help Ukraine Inc | Fairfax | P20 Human Services | $18.5M | 2 |
| RICHMOND METROPOLITAN CONVENTION & | Richmond | — | $18.5M | 6 |
| PQA INC | Alexandria | E80 Health Care | $18.4M | 6 |
| National Beer Wholesalers Association | Alexandria | — | $18.3M | 7 |
| FUTURE BUSINESS LEADERS OF AMERICA | Reston | — | $18.3M | 0 |
| TRAGEDY ASSISTANCE PROGRAM FOR | Arlington | F600 Mental Health & Crisis Intervention | $18.2M | 5 |
| THE OAK HILL FUND | Charlottesville | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $18.2M | 2 |
| PATHWAY HOMES INC | Fairfax | F33Z Mental Health & Crisis Intervention | $18.2M | 8 |
| VIRGINIA PROPERTY INSURANCE ASSN | Glen Allen | — | $18.1M | 2 |
| HUMAN RESOURCE CERTIFICATION | Alexandria | — | $18.1M | 9 |
| NATIONAL ASSOCIATION FOR COLLEGE | Arlington | B90 Education | $18.1M | 11 |
| CHESAPEAKE REGIONAL MEDICAL GROUP | Chesapeake | E11 Health Care | $18M | 7 |
| CROPLIFE AMERICA | Arlington | — | $18M | 12 |
| THE COVENANT SCHOOL INC | Charlottesville | — | $18M | 7 |
| FEDEX PILOTS POST-MEDICARE RETIREE PREMIUM | McLean | Y43 Mutual & Membership Benefit | $18M | 6 |
| VSE CORPORATION GROUP | Alexandria | Y42 Mutual & Membership Benefit | $17.9M | 0 |
| AMERICAN INDUSTRIAL HYGIENE ASSOCIATION | Falls Church | — | $17.8M | 14 |
| GLOBAL ENTREPRENEURSHIP NETWORK | Arlington | B99 Education | $17.8M | 2 |
| UNIVERSITY OF VIRGINIA HEALTH FOUNDATION | Charlottesville | B114 Education | $17.7M | 1 |
| COMMUNITY ASSOCIATIONS INSTITUTE | Falls Church | — | $17.7M | 11 |
| Students for Life of America Inc | Fredericksburg | R620 Civil Rights, Social Action & Advocacy | $17.6M | 4 |
| PORTLAND CEMENT ASSOCIATION | Alexandria | — | $17.5M | 7 |
| Foxcroft School | Middleburg | B250 Education | $17.5M | 5 |
| PENINSULA INSTITUTE FOR COMMUNITY | Newport News | E320 Health Care | $17.5M | 9 |
| ASCO ASSOCIATION | Alexandria | S41 Community Improvement & Capacity Building | $17.4M | 10 |
| WESTWOOD COUNTRY CLUB INC | Vienna | — | $17.4M | 5 |
| VIRGINIA INSTITUTE OF AUTISM INC | Charlottesville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $17.3M | 1 |
| FRIENDSHIP HEALTH AND REHAB CENTER SOUTH | Roanoke | E91 Health Care | $17.2M | 9 |
| CRAIG-BOTETOURT ELECTRIC | New Castle | — | $17.1M | 9 |
| RICHMOND EYE AND EAR HEALTHCARE ALLIANCE | Richmond | E24 Health Care | $17.1M | 9 |
| HIGHLAND SCHOOL INC | Warrenton | P20 Human Services | $17M | 7 |
| HOME SCHOOL LEGAL DEFENSE ASSOCIATION | Purcellville | — | $17M | 10 |
| INTERNATIONAL TRAINING INSTITUTE FOR THE | Falls Church | — | $16.9M | 7 |
| BOULDER CREST FOUNDATION | Bluemont | P80 Human Services | $16.9M | 8 |
| The J127 Education Foundation | Fairfax | B200 Education | $16.8M | 7 |
| WESTMINSTER CANTERBURY MANAGEMENT | Richmond | P110 Human Services | $16.8M | 13 |
| THE CAMPAGNA CENTER INC | Alexandria | P330 Human Services | $16.8M | 5 |
| CAPITAL AREA HEALTH NETWORK | Richmond | E32 Health Care | $16.7M | 10 |
| AMERICAN MEDICAL GROUP | Alexandria | — | $16.7M | 9 |
| HAMPTON ROADS EDUCATIONAL | Norfolk | A32 Arts, Culture & Humanities | $16.7M | 8 |
| GREATER CHARLOTTESVILLE HABITAT | Charlottesville | — | $16.7M | 5 |
| SOCIETY OF NUCLEAR MEDICINE AND | Reston | G96 Diseases, Disorders & Medical Disciplines | $16.7M | 10 |
| TRINITY EPISCOPAL SCHOOL | Richmond | B24Z Education | $16.7M | 2 |
| Children of Fallen Patriots Foundation | Reston | B82 Education | $16.7M | 6 |
