| GLOBAL BUSINESS TRAVEL ASSOCIATION (GBTA) INC | Alexandria | — | $19.5M | 10 |
| GLOBAL ENVIRONMENT & TECHNOLOGY FOUNDATION | Arlington | C60Z Environment | $19.5M | 4 |
| SHEET METAL AND AIR CONDITIONING CONTRACTORS' NATIONAL ASSOCIATION | Chantilly | — | $19.5M | 7 |
| PEOPLE INCORPORATED OF VIRGINIA | Abingdon | — | $19.4M | 5 |
| Vertical Aviation International Inc | Alexandria | — | $19.4M | 9 |
| GOODWILL INDUSTRIES OF THE VALLEYS INC | Roanoke | J320 Employment | $19.3M | 15 |
| Centra Health Foundation | Lynchburg | E110 Health Care | $19.3M | 5 |
| CULPEPER BAPTIST RETIREMENT COMMUNITY INC | Glen Allen | P75Z Human Services | $19.3M | 12 |
| RICHMOND METROPOLITAN CONVENTION & VISTOR'S BUREAU | Richmond | — | $19.2M | 7 |
| BLUE EAGLE CREDIT UNION | Roanoke | — | $19.2M | 5 |
| THE COVENANT SCHOOL INC | Charlottesville | — | $19.1M | 6 |
| CITIZENS TELEPHONE COOPERATIVE | Floyd | Y22 Mutual & Membership Benefit | $19.1M | 13 |
| MID-ATLANTIC BROADBAND COMMUNITIES CORPORATION | South Boston | S32 Community Improvement & Capacity Building | $19M | 23 |
| THE MILLER SCHOOL OF ALBEMARLE | Charlottesville | B99 Education | $19M | 3 |
| INTERNATIONAL ASSOCIATION OF BETTER BUSINESS BUREAUS INC | Arlington | P20 Human Services | $18.9M | 10 |
| THE SALVATION ARMY WORLD SERVICE OFFICE | Alexandria | Q300 International, Foreign Affairs & National Security | $18.9M | 8 |
| Shelter House Inc | Fairfax | L20 Housing & Shelter | $18.7M | 1 |
| NRECA INTERNATIONAL | Arlington | — | $18.7M | 12 |
| National Beer Wholesalers Association Inc | Alexandria | — | $18.7M | 7 |
| CROSSROADS COMMUNITY SERVICES BOARD | Farmville | F32Z Mental Health & Crisis Intervention | $18.7M | 7 |
| The Joshua Fund | McLean | X99 Religion-Related | $18.6M | 6 |
| THE BLUE RIDGE SCHOOL INC | St George | B20I Education | $18.6M | 5 |
| NATIONAL SCIENCE TEACHING ASSOCIATION | Richmond | B030 Education | $18.6M | 15 |
| United Help Ukraine Inc | Fairfax | P20 Human Services | $18.5M | 2 |
| RAINFOREST TRUST | Warrenton | Q300 International, Foreign Affairs & National Security | $18.5M | 4 |
| TRI-AREA COMMUNITY HEALTH | Laurel Fork | E32Z Health Care | $18.5M | 8 |
| PQA INC | Alexandria | E80 Health Care | $18.4M | 6 |
| THE OAK HILL FUND | Charlottesville | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $18.2M | 2 |
| VIRGINIA PROPERTY INSURANCE ASSN | Glen Allen | — | $18.1M | 2 |
| HUMAN RESOURCE CERTIFICATION INSTITUTE INC | Alexandria | — | $18.1M | 9 |
| NATIONAL ASSOCIATION FOR COLLEGE ADMISSION COUNSELING | Arlington | B90 Education | $18.1M | 11 |
| Liberty Christian Academy | Lynchburg | — | $18M | 8 |
| CROPLIFE AMERICA | Arlington | — | $18M | 12 |
| FEDEX PILOTS POST-MEDICARE RETIREE PREMIUM REIMBURSEMENT PLAN | McLean | Y43 Mutual & Membership Benefit | $18M | 6 |
| DISABILITYIN | Alexandria | S112 Community Improvement & Capacity Building | $17.9M | 7 |
| AMERICAN INDUSTRIAL HYGIENE ASSOCIATION | Falls Church | — | $17.8M | 14 |
| GLOBAL ENTREPRENEURSHIP NETWORK | Arlington | B99 Education | $17.8M | 2 |
| CENTRAL FAIRFAX SERVICES INC | Springfield | P80 Human Services | $17.8M | 11 |
| Students for Life of America Inc | Fredericksburg | R620 Civil Rights, Social Action & Advocacy | $17.6M | 4 |
| AMERICAN CEMENT ASSOCIATION | Alexandria | — | $17.6M | 7 |
| Foxcroft School | Middleburg | B250 Education | $17.5M | 5 |
| ASCO ASSOCIATION | Alexandria | S41 Community Improvement & Capacity Building | $17.4M | 10 |
| WESTWOOD COUNTRY CLUB INC | Vienna | — | $17.4M | 5 |
| AMERICAN TRAFFIC SAFETY SERVICES ASSOCIATION INC | Fredericksburg | — | $17.3M | 13 |
| FRIENDSHIP HEALTH AND REHAB CENTER SOUTH | Roanoke | E91 Health Care | $17.2M | 9 |
| CRAIG-BOTETOURT ELECTRIC COOPERATIVE | New Castle | — | $17.1M | 9 |
| NORTH CROSS SCHOOL | Roanoke | B200 Education | $17.1M | 4 |
| HIGHLAND SCHOOL INC | Warrenton | P20 Human Services | $17M | 7 |
| HOME SCHOOL LEGAL DEFENSE ASSOCIATION | Purcellville | — | $17M | 10 |
| STAND WITH CRYPTO ALLIANCE INC | McLean | — | $17M | 4 |
