| SECOND HARVEST FOOD BANK OF MIDDLE TN | Nashville | K300 Food, Agriculture & Nutrition | $150M | 9 |
| CENTERSTONE OF TENNESSEE INC | Nashville | F99 Mental Health & Crisis Intervention | $149.7M | 9 |
| Morristown-Hamblen Hospital Association | Knoxville | E220 Health Care | $140.1M | 11 |
| MTPC LLC | Knoxville | E24 Health Care | $139.8M | 0 |
| DCI DONOR SERVICES INC | Nashville | E65Z Health Care | $136M | 16 |
| Wellmont Medical Associates Inc | Johnson City | E30 Health Care | $135.9M | 22 |
| MERIWETHER LEWIS ELECTRIC | Centerville | — | $133.2M | 26 |
| SOUTHWEST TENNESSEE ELECTRIC | Brownsville | — | $132.8M | 20 |
| HCA SHORT TERM DISABILITY BENEFITS PLAN TRUST | Nashville | Y43 Mutual & Membership Benefit | $129.7M | 1 |
| UPPER CUMBERLAND ELECTRIC | Carthage | W80 Public & Societal Benefit | $128.2M | 24 |
| THE MCDONNELL FAMILY FOUNDATION | Memphis | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $127.4M | 0 |
| GIBSON ELECTRIC MEMBERSHIP | Trenton | — | $125.5M | 22 |
| OAK RIDGE ASSOCIATED UNIVERSITIES INC | Oak Ridge | — | $124.4M | 36 |
| MEMORIAL HEALTH PARTNERS FOUNDATION INC | Chattanooga | — | $121.6M | 20 |
| TENNESSEE PERFORMING ARTS CENTER (TPAC) | Nashville | A610 Arts, Culture & Humanities | $119.4M | 11 |
| PCPT HAMLIN LLC | Knoxville | G30 Diseases, Disorders & Medical Disciplines | $116.2M | 0 |
| NATIONAL FEDERATION OF INDEPENDENT | Nashville | — | $115.8M | 27 |
| CHRISTIAN COMMUNITY FOUNDATION OF | Memphis | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $112.2M | 3 |
| SEQUACHEE VALLEY ELECTRIC | South Pittsburg | — | $112M | 20 |
| Goodwill Industries of Middle TN Inc | Nashville | J32 Employment | $111.8M | 10 |
| HELEN ROSS MCNABB CENTER INC | Knoxville | F30Z Mental Health & Crisis Intervention | $111.4M | 8 |
| COMMUNITY FOUNDATION | Memphis | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $110.8M | 7 |
| BILL AND CRISSY HASLAM FOUNDATION | Knoxville | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $109.7M | 0 |
| SOLES4SOULS INC | Old Hickory | P60 Human Services | $108.4M | 9 |
| Takoma Regional Hospital Inc | Johnson City | E200 Health Care | $107.3M | 18 |
| HEALTH SERVICES MANAGEMENT INC | Murfreesboro | E91 Health Care | $107.1M | 16 |
| Union University | Jackson | B420 Education | $104.8M | 7 |
| Holston Electric Cooperative Inc | Rogersville | — | $101.4M | 14 |
| CHEROKEE HEALTH SYSTEMS | Knoxville | E32 Health Care | $98.9M | 9 |
| Young Men's Christian Association of Middle Tennessee (6273) | Nashville | P270 Human Services | $98.3M | 15 |
| CHARITY GLOBAL INC | Franklin | P80 Human Services | $95M | 14 |
| Cumberland Medical Center | Knoxville | E220 Health Care | $94.3M | 13 |
| FORT LOUDOUN ELECTRIC COOPERATIVE | Vonore | — | $93.2M | 21 |
| LEE UNIVERSITY | Cleveland | — | $91.6M | 9 |
| Trevecca Nazarene University | Nashville | B420 Education | $90.7M | 10 |
| OCOEE FOUNDATION INC | Cleveland | E91 Health Care | $90M | 13 |
| THE DOLLYWOOD FOUNDATION | Sevierville | — | $89.8M | 5 |
| UT REGIONAL ONE PHYSICIANS INC | Memphis | E24 Health Care | $88.4M | 17 |
| SCARLETT FAMILY FOUNDATION | Nashville | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $86.8M | 2 |
| MECHANICAL LICENSING COLLECTIVE | Nashville | J02 Employment | $84.7M | 15 |
| THE HASLAM 3 FOUNDATION INC | Knoxville | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $84.3M | 0 |
| FRONTIER HEALTH | Gray | E32 Health Care | $83.4M | 6 |
| MENTAL HEALTH COOPERATIVE INC | Nashville | F32Z Mental Health & Crisis Intervention | $83.1M | 8 |
| YOUTH VILLAGES FOUNDATION INC | Memphis | P11 Human Services | $82.5M | 8 |
| GARY SINISE FOUNDATION | Nashville | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $81.4M | 17 |
| VOLUNTEER BEHAVIORAL HEALTH CARE | Chattanooga | F30Z Mental Health & Crisis Intervention | $81M | 27 |
| UT Medical Group Inc | Memphis | E300 Health Care | $80.8M | 21 |
| DAVIDSON TRANSIT ORGANIZATION | Madison | — | $79.6M | 12 |
| CANEY FORK ELECTRIC COOPERATIVE INC | McMinnville | — | $77.4M | 21 |
| CUMBERLAND UNIVERSITY | Lebanon | — | $75.7M | 11 |
