| SECOND HARVEST FOOD BANK OF MIDDLE TN INC | Nashville | K300 Food, Agriculture & Nutrition | $156.6M | 8 |
| EDUCATION LOAN FINANCE INC | Knoxville | B82 Education | $152.8M | 19 |
| VOLUNTEER CORPORATE CREDIT UNION | Nashville | — | $150.2M | 10 |
| CENTERSTONE OF TENNESSEE INC | Nashville | F99 Mental Health & Crisis Intervention | $149.7M | 9 |
| TRI-COUNTY ELECTRIC MEMBERSHIP CORPORATION | Lafayette | — | $147.3M | 14 |
| Wellmont Medical Associates Inc | Johnson City | E30 Health Care | $144.2M | 24 |
| UPPER CUMBERLAND ELECTRIC MEMBERSHIP CORPORATION | Carthage | W80 Public & Societal Benefit | $143M | 23 |
| OPERATION COMPASSION | Cleveland | X20 Religion-Related | $140.3M | 1 |
| Morristown-Hamblen Hospital Association | Knoxville | E220 Health Care | $140.1M | 11 |
| MTPC LLC | Knoxville | E24 Health Care | $139.8M | 0 |
| APPALACHIAN ELECTRIC COOPERATIVE | New Market | — | $139.1M | 17 |
| SOUTHWEST TENNESSEE ELECTRIC MEMBERSHIP CORPORATION | Brownsville | — | $136.9M | 26 |
| DCI DONOR SERVICES INC | Nashville | E65Z Health Care | $136M | 16 |
| MEMORIAL HEALTH PARTNERS FOUNDATION INC | Chattanooga | — | $135.4M | 21 |
| MERIWETHER LEWIS ELECTRIC COOPERATIVE | Centerville | — | $133.2M | 26 |
| HCA SHORT TERM DISABILITY BENEFITS PLAN TRUST | Nashville | Y43 Mutual & Membership Benefit | $129.7M | 1 |
| SOLES4SOULS INC | Old Hickory | P60 Human Services | $126.2M | 11 |
| GIBSON ELECTRIC MEMBERSHIP | Trenton | — | $125.5M | 22 |
| OAK RIDGE ASSOCIATED UNIVERSITIES INC | Oak Ridge | — | $124.4M | 36 |
| Health Services Management Inc | Murfreesboro | E91 Health Care | $121.3M | 14 |
| HELEN ROSS MCNABB CENTER INC | Knoxville | F30Z Mental Health & Crisis Intervention | $121.1M | 8 |
| THE TROUSDALE FOUNDATION INC | Cleveland | — | $120.8M | 6 |
| PCPT HAMLIN LLC | Knoxville | G30 Diseases, Disorders & Medical Disciplines | $116.2M | 0 |
| NATIONAL FEDERATION OF INDEPENDENT BUSINESS INC | Nashville | — | $115.8M | 27 |
| CHRISTIAN COMMUNITY FOUNDATION OF MEMPHIS | Memphis | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $112.2M | 3 |
| SEQUACHEE VALLEY ELECTRIC COOPERATIVE | South Pittsburg | — | $112M | 20 |
| Goodwill Industries of Middle TN Inc | Nashville | J32 Employment | $111.8M | 10 |
| MECHANICAL LICENSING COLLECTIVE | Nashville | J02 Employment | $111.3M | 16 |
| Takoma Regional Hospital Inc | Johnson City | E200 Health Care | $110.8M | 22 |
| Holston Electric Cooperative Inc | Rogersville | — | $110.2M | 14 |
| BILL AND CRISSY HASLAM FOUNDATION | Knoxville | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $109.7M | 0 |
| Cumberland Medical Center | Knoxville | E220 Health Care | $105.2M | 11 |
| Union University | Jackson | B420 Education | $104.8M | 7 |
| CHEROKEE HEALTH SYSTEMS | Knoxville | E32 Health Care | $98.9M | 9 |
| Young Men's Christian Association of Middle Tennessee (6273) | Nashville | P270 Human Services | $98.3M | 15 |
| THE PROTON THERAPY CENTER LLC | Knoxville | E24 Health Care | $98.3M | 0 |
| THE BILL GATTON FOUNDATION | Bristol | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $97.5M | 2 |
| TREVECCA NAZARENE UNIVERSITY | Nashville | B420 Education | $95.9M | 15 |
| CHARITY GLOBAL INC | Franklin | P80 Human Services | $95M | 14 |
| Leaders Credit Union | Jackson | W61 Public & Societal Benefit | $93.3M | 9 |
| FORT LOUDOUN ELECTRIC COOPERATIVE | Vonore | — | $93.2M | 21 |
| UT Regional One Physicians Inc | Memphis | E24 Health Care | $93.1M | 15 |
| MENTAL HEALTH COOPERATIVE INC | Nashville | F32Z Mental Health & Crisis Intervention | $91.8M | 8 |
| LEE UNIVERSITY | Cleveland | — | $91.6M | 9 |
| OCOEE FOUNDATION INC | Cleveland | E91 Health Care | $90M | 13 |
| THE DOLLYWOOD FOUNDATION | Sevierville | — | $89.8M | 5 |
| SCARLETT FAMILY FOUNDATION | Nashville | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $86.8M | 2 |
| UT LE BONHEUR PEDIATRIC SPECIALISTS INC | Memphis | E300 Health Care | $85.7M | 20 |
| CANEY FORK ELECTRIC COOPERATIVE INC | McMinnville | — | $85M | 18 |
| FRONTIER HEALTH | Gray | E32 Health Care | $83.4M | 6 |
