| MID-SOUTH FOOD BANK | Memphis | K31Z Food, Agriculture & Nutrition | $81.8M | 4 |
| GARY SINISE FOUNDATION | Nashville | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $81.4M | 17 |
| VOLUNTEER BEHAVIORAL HEALTH CARE SYSTEM | Chattanooga | F30Z Mental Health & Crisis Intervention | $81M | 27 |
| Ballad Health | Johnson City | E19 Health Care | $81M | 17 |
| UT Medical Group Inc | Memphis | E300 Health Care | $80.8M | 21 |
| COUNTRY MUSIC ASSOCIATION INC | Nashville | A68Z Arts, Culture & Humanities | $78.2M | 10 |
| CUMBERLAND UNIVERSITY | Lebanon | — | $75.7M | 11 |
| Sweetwater Hospital Association | Sweetwater | E22Z Health Care | $75.4M | 19 |
| POWELL VALLEY ELECTRIC COOPERATIVE | New Tazewell | — | $74.4M | 16 |
| TWIN LAKES TELEPHONE COOPERATIVE CORP | Gainesboro | — | $74.3M | 18 |
| FIRST HORIZON CORPORATION EMPLOYEES' HEALTHCARE AND WELFARE TRUST | Memphis | — | $73.3M | 0 |
| DAVIDSON TRANSIT ORGANIZATION | Madison | — | $73.1M | 15 |
| Wellmont Cardiology Services | Johnson City | E30 Health Care | $72.5M | 27 |
| SHEET METAL WORKERS NATIONAL HEALTH FUND | Goodletsville | — | $72.5M | 1 |
| MOUNTAIN ELECTRIC COOPERATIVE INC | Mountain City | — | $71.6M | 10 |
| ALLIANCE HEALTHCARE SERVICES | Memphis | — | $71.6M | 9 |
| TENNESSEE LIFE & HEALTH INSURANCE GUARANTY ASSOCIATION | Nashville | — | $71.1M | 0 |
| THE ENSWORTH SCHOOL | Nashville | B240 Education | $71M | 7 |
| KIPP NASHVILLE | Whites Creek | B29 Education | $70.8M | 10 |
| FREED-HARDEMAN UNIVERSITY | Henderson | B420 Education | $69.2M | 10 |
| CHRISTIAN BROTHERS UNIVERSITY | Memphis | B430 Education | $68.8M | 7 |
| MEDICAL EDUCATION ASSISTANCE CORP | Mountain Home | — | $68M | 30 |
| BAYLOR SCHOOL | Chattanooga | B250 Education | $67.4M | 13 |
| SOUTHEAST COMMUNITY CAPITAL CORPORATION D/B/A PATHWAY LENDING | Nashville | J20 Employment | $65.1M | 14 |
| OPEN ARMS CARE CORPORATION | Brentwood | P730 Human Services | $64.6M | 6 |
| MARYVILLE COLLEGE | Maryville | B420 Education | $64.3M | 11 |
| THE MCCALLIE SCHOOL | Chattanooga | B200 Education | $64.2M | 11 |
| Ducks Unlimited Inc Group Return | Memphis | C300 Environment | $63.2M | 13 |
| NASHVILLE CARES | Nashville | G81Z Diseases, Disorders & Medical Disciplines | $63.2M | 3 |
| CHRISTIAN ADVOCATES SERVING EVANGELISM INC | Brentwood | R600 Civil Rights, Social Action & Advocacy | $62.7M | 11 |
| YMCA OF MEMPHIS & THE MID-SOUTH | Cordova | P27Z Human Services | $62.2M | 6 |
| First Horizon Foundation | Memphis | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $62.2M | 1 |
| Christ Community Health Services Inc | Memphis | E39Z Health Care | $61.6M | 12 |
| FISK UNIVERSITY | Nashville | B43 Education | $61.4M | 21 |
| THE STERN CARDIOVASCULAR FOUNDATION INC | Germantown | E32 Health Care | $61.3M | 20 |
| UNITED FURNITURE WORKERS INSURANCE FUND | Nashville | — | $60.2M | 1 |
| MUSIC CITY PREP CLINIC | Nashville | E32 Health Care | $59.5M | 2 |
| CARSON-NEWMAN UNIVERSITY | Jefferson City | B420 Education | $58.7M | 13 |
| MILLIGAN UNIVERSITY | Milligan | B42Z Education | $58.3M | 11 |
| Vine International Inc | Knoxville | E60Z Health Care | $58.2M | 3 |
| COUNTRY MUSIC FOUNDATION INC | Nashville | A500 Arts, Culture & Humanities | $57.1M | 9 |
| LEAD PUBLIC SCHOOLS INC | Brentwood | B29 Education | $56.4M | 8 |
| YOUTH VILLAGES FOUNDATION INC | Memphis | P11 Human Services | $56.3M | 8 |
| UNIVERSITY OF CHATTANOOGA FOUNDATION INC | Chattanooga | B11 Education | $56M | 0 |
| SISKIN HOSPITAL FOR PHYSICAL REHABILITATION INC | Chattanooga | E220 Health Care | $55M | 13 |
| THE HASLAM 3 FOUNDATION INC | Knoxville | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $54.9M | 0 |
| BAPTIST MEMORIAL HOSPITAL-UNION CITY INC | Union City | E220 Health Care | $54.8M | 13 |
| SOUTHERN OPERATORS HEALTH FUND | Goodlettsville | Y43 Mutual & Membership Benefit | $54.6M | 0 |
| TENNESSEE PERFORMING ARTS CENTER (TPAC) MANAGEMENT CORPORATION | Nashville | A610 Arts, Culture & Humanities | $54.2M | 11 |
| UNITY MEDICAL CENTER INC | Manchester | E22 Health Care | $54.1M | 14 |
