| AMERICAN MEDICAL ASSOCIATION | Chicago | — | $468.4M | 40 |
| METHODIST MEDICAL CENTER OF ILLINOIS | Urbana | E220 Health Care | $462.9M | 25 |
| Riverside Medical Center | Kankakee | E220 Health Care | $457.5M | 19 |
| University of Illinois Foundation | Champaign | B110 Education | $450.3M | 19 |
| Rehabilitation Institute of Chicago | Chicago | E240 Health Care | $446.7M | 10 |
| Illinois Institute of Technology | Chicago | B430 Education | $444M | 28 |
| ALZHEIMER'S DISEASE & RELATED DISORDERS ASSOCIATION INC | Chicago | G830 Diseases, Disorders & Medical Disciplines | $443.9M | 13 |
| BAXTER CREDIT UNION | Vernon Hills | W61 Public & Societal Benefit | $443.9M | 0 |
| UNITED AIRLINES EMPLOYEE SICK LEAVE TRUST | Chicago | Y43 Mutual & Membership Benefit | $443.7M | 0 |
| MOUNT SINAI HOSPITAL MEDICAL CENTER | Chicago | E220 Health Care | $426.4M | 15 |
| THE ROTARY FOUNDATION OF ROTARY INTERNATIONAL | Evanston | Q113 International, Foreign Affairs & National Security | $418.1M | 17 |
| CHRISTIAN BROTHERS EMPLOYEE BENEFIT TRUST | Romeoville | — | $414.4M | 0 |
| Javon Bea Hospital | Rockford | E220 Health Care | $399.2M | 11 |
| Copley Memorial Hospital Inc | Aurora | E220 Health Care | $399.2M | 17 |
| NATIONAL OPINION RESEARCH CENTER | Chicago | V200 Social Science | $397.2M | 23 |
| HOWARD G BUFFETT FOUNDATION | Decatur | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $377.6M | 2 |
| CITIZENS EQUITY FIRST CREDIT UNION | Peoria | W61 Public & Societal Benefit | $367.7M | 20 |
| ELECTRICAL INSURANCE TRUSTEES | Alsip | — | $361.1M | 10 |
| The Art Institute of Chicago | Chicago | B400 Education | $359.5M | 22 |
| National Association of Realtors | Chicago | — | $358.2M | 20 |
| Swedish Covenant Health | Chicago | — | $352.3M | 15 |
| AHS MIDWEST MANAGEMENT INC | La Grange | — | $349.3M | 13 |
| Hospital Sisters Health System | Springfield | E210 Health Care | $339.3M | 10 |
| EXELON CORPORATION EMPLOYEES' BENEFIT TRUST C/O ACCOUNTING OPERATIONS | Chicago | — | $336.6M | 1 |
| The Ingalls Memorial Hospital | Harvey | E220 Health Care | $326.1M | 18 |
| MID-AMERICA CARPENTERS REGIONAL COUNCIL HEALTH FUND | Chicago | — | $324.1M | 14 |
| Carle Health Care Incorporated | Urbana | E21Z Health Care | $323.6M | 23 |
| PERT Foundation | Chicago | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $311.7M | 3 |
| ILLINOIS NETWORK OF CHILD CARE RESOURCE AND REFERRAL AGENCIES | Bloomington | P99 Human Services | $300M | 6 |
| College of American Pathologists | Northfield | — | $299.5M | 36 |
| St Elizabeth's Hospital of the Hospital Sisters of the Third Order of St Fr ancis | Ofallon | E220 Health Care | $297.2M | 9 |
| GREATER CHICAGO FOOD DEPOSITORY | Chicago | K31Z Food, Agriculture & Nutrition | $275.9M | 11 |
| INTERNATIONAL FELLOWSHIP OF CHRISTIANS & JEWS INC | Chicago | — | $271.5M | 10 |
| Ottawa Regional Hospital & Healthcare Center | Peoria | E220 Health Care | $269.6M | 47 |
| Pediatric Faculty Foundation Inc | Chicago | E20 Health Care | $267.5M | 11 |
| CHICAGO AND VICINITY LABORERS' DISTRICT COUNCIL HEALTH & WELFARE PLAN | Westchester | — | $263.9M | 8 |
| ADVENTIST MIDWEST HEALTH | Hinsdale | E220 Health Care | $262.8M | 0 |
| GOTTLIEB COMMUNITY HEALTH SERVICES CORPORATION | Melrose Park | E22 Health Care | $257.8M | 19 |
| CONSUMERS CREDIT UNION | Lake Forest | — | $255.3M | 12 |
| Morris Hospital | Morris | E220 Health Care | $251.1M | 15 |
| Columbia College Chicago | Chicago | B42 Education | $250.7M | 13 |
| Carle BroMenn Medical Center | Urbana | E20 Health Care | $250.6M | 22 |
| ONE ACRE FUND | Highland Park | Q30 International, Foreign Affairs & National Security | $248.2M | 11 |
| NOBLE NETWORK OF CHARTER SCHOOLS | Chicago | B24 Education | $240.9M | 10 |
| American Dental Association | Chicago | — | $237.1M | 54 |
| HUNTER FAMILY FOUNDATION C/O FINANCIAL INVESTMENT CORP | Chicago | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $234.1M | 0 |
| ANDERSON HOSPITAL | Maryville | E200 Health Care | $229.2M | 10 |
| MEMORIAL HEALTH SYSTEM | Springfield | E21 Health Care | $226.8M | 16 |
| BRADLEY UNIVERSITY | Peoria | B430 Education | $226.4M | 15 |
| COMMUNITY AND ECONOMIC DEVELOPMENT ASSOCIATION OF COOK COUNTY INC | Chicago | S20 Community Improvement & Capacity Building | $215.2M | 8 |
