| COMMUNITY CARE OF NORTH CAROLINA INC | Garner | E21 Health Care | $233M | 14 |
| NORTH CAROLINA JOINT UNDERWRITING ASSOCIATION | Raleigh | — | $232.8M | 11 |
| CAMPBELL UNIVERSITY | Buies Creek | B430 Education | $231.3M | 15 |
| DUKE UNIVERSITY AFFILIATED PHYSICIANS INC | Durham | E300 Health Care | $230.5M | 16 |
| AMERICAN INSTITUTE OF CERTIFIED PUBLIC ACCOUNTANTS | Durham | — | $227.8M | 3 |
| ASSOCIATION OF INTERNATIONAL CERTIFIED PROFESSIONAL ACCOUNTANTS | Durham | S41 Community Improvement & Capacity Building | $223M | 12 |
| The Bank of America Charitable Foundation Inc | Charlotte | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $218.8M | 0 |
| AH GEORGIA INC | Charlotte | E60 Health Care | $213.6M | 3 |
| Caldwell Memorial Hospital Inc | Lenoir | E220 Health Care | $207.5M | 18 |
| UNION ELECTRIC MEMBERSHIP CORP | Monroe | — | $204.9M | 17 |
| Second Harvest Food Bank of Metrolina Inc | Charlotte | K31 Food, Agriculture & Nutrition | $170.3M | 5 |
| LUMBEE RIVER ELEC MEMBERSHIP CORP | Pembroke | — | $169.6M | 20 |
| RUTHERFORD ELECTRIC MEMBERSHIP CORPORATION | Forest City | — | $167.7M | 16 |
| CAROMONT MEDICAL GROUP INC | Gastonia | E11 Health Care | $165.7M | 11 |
| Lenoir Memorial Hospital Inc | Kinston | E220 Health Care | $165.7M | 11 |
| NORTH CAROLINA STATE UNIVERSITY FOUNDATION INC | Raleigh | B110 Education | $164.9M | 8 |
| Wingate University | Wingate | B430 Education | $164.2M | 11 |
| WAKE FOREST UNIVERSITY BAPTIST MEDICAL CENTER | Winstonsalem | E120 Health Care | $163.4M | 18 |
| BLUE RIDGE ELECTRIC MEMBERSHIP CORP | Lenoir | — | $162M | 27 |
| Columbus Regional Healthcare System | Whiteville | E22 Health Care | $161.4M | 9 |
| JONES-ONSLOW ELECTRIC MEMBERSHIP CORPORATION | Jacksonville | — | $159.9M | 25 |
| MANA NUTRITIVE AID PRODUCTS INCORPORATED | Matthews | Q33 International, Foreign Affairs & National Security | $159.1M | 5 |
| LEXINGTON MEDICAL CENTER | Lexington | E220 Health Care | $153.3M | 10 |
| DELTA DENTAL OF NORTH CAROLINA | Raleigh | — | $152.3M | 18 |
| ATRIUM HEALTH FOUNDATION | Charlotte | E12 Health Care | $150.1M | 9 |
| The Outer Banks Hospital Inc | Greenville | E200 Health Care | $146.6M | 11 |
| WRMC HOSPITAL OPERATING CORPORATION | North Wilkesboro | E20 Health Care | $143.8M | 14 |
| THE DUKE ENDOWMENT | Charlotte | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $142M | 19 |
| DAVIE MEDICAL CENTER | Bermuda Run | E20 Health Care | $141.3M | 12 |
| HUGH CHATHAM MEMORIAL HOSPITAL INC | Elkin | E220 Health Care | $139.5M | 11 |
| LEGACY HEALTHCARE SERVICES INC | Raleigh | E60 Health Care | $139.3M | 14 |
| The Young Men's Christian Association of the Triangle Area Inc (4598) | Raleigh | P270 Human Services | $138.9M | 12 |
| CENTER FOR CREATIVE LEADERSHIP | Greensboro | B60 Education | $136.9M | 39 |
| GASTON FAMILY HEALTH SERVICES INC | Gastonia | — | $135.1M | 16 |
| MOUNTAIN AREA HEALTH EDUCATION CENTER INC | Asheville | B54Z Education | $129.2M | 8 |
| NORTH CAROLINA PARTNERSHIP FOR CHILDREN INC | Raleigh | B012 Education | $123.9M | 5 |
| CATAWBA COLLEGE | Salisbury | B420 Education | $119.8M | 12 |
| WAKE FOREST EMERGENCY PROVIDERS | Winstonsalem | E31Z Health Care | $118.8M | 7 |
| WAKE ELECTRIC MEMBERSHIP CORPORATION | Wake Forest | — | $116.7M | 15 |
| MONARCH | Albemarle | P40 Human Services | $115.2M | 12 |
| FOUR COUNTY ELECTRIC MEMBERSHIP CORP | Burgaw | — | $115M | 18 |
| Hendrick Automotive Group Voluntary Employees' Beneficiary Association Trus t | Charlotte | — | $114.7M | 0 |
| SOUTH RIVER ELECTRIC MEMBERSHIP CORPORATION | Dunn | — | $113.2M | 15 |
| C D SPANGLER FOUNDATION INC | Charlotte | T22Z Philanthropy, Voluntarism & Grantmaking Foundations | $112.3M | 0 |
| New Hanover Community Endowment Inc | Wilmington | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $111.7M | 8 |
| ALAMANCE REGIONAL MEDICAL CENTER INC | Greensboro | E220 Health Care | $107.5M | 21 |
| WAKE FOREST UNIVERSITY HEALTH SCIENCES GROUP RETURN | Winstonsalem | B50 Education | $106.7M | 9 |
| CITY OF OAKS FOUNDATION INC | Raleigh | C40 Environment | $105.2M | 1 |
| EASTER SEALS UCP NORTH CAROLINA & VIRGINIA INC | Raleigh | E125 Health Care | $104.2M | 18 |
| THE GIVENS ESTATES INC | Asheville | P750 Human Services | $103.4M | 10 |
