| GARDNER-WEBB UNIVERSITY | Boiling Springs | B43 Education | $97.3M | 8 |
| RISE AGAINST HUNGER INC | Raleigh | Q330 International, Foreign Affairs & National Security | $94.5M | 11 |
| GOODWILL INDUSTRIES OF THE SOUTHERN | Charlotte | J32Z Employment | $93.5M | 11 |
| Queens University of Charlotte | Charlotte | B430 Education | $91.8M | 14 |
| THE CROSLAND FOUNDATION | Charlotte | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $90M | 2 |
| GOODWILL INDUSTRIES OF NORTHWEST NORTH CAROLINA INC | Winstonsalem | J32Z Employment | $90M | 9 |
| MOSES CONE PHYSICIAN SERVICES INC | Greensboro | E30 Health Care | $89.4M | 11 |
| MOSES CONE AFFILIATED PHYSICIANS INC | Greensboro | E21 Health Care | $89.3M | 11 |
| COTTON INCORPORATED | Cary | — | $89.1M | 14 |
| FRENCH BROAD ELECTRIC MEMBERSHIP | Marshall | — | $87.8M | 21 |
| HOSPICE & PALLIATIVE CARE CHARLOTTE | Charlotte | P740 Human Services | $87.7M | 11 |
| BISSELL BALLANTYNE LEGACY FOUNDATION | Charlotte | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $86.2M | 1 |
| MEREDITH COLLEGE | Raleigh | B430 Education | $85.9M | 11 |
| SECOND HARVEST FOOD BANK | Winston Salem | K31Z Food, Agriculture & Nutrition | $85.8M | 6 |
| Lenoir-Rhyne University | Hickory | B420 Education | $83.7M | 12 |
| PIEDMONT HEALTH SERVICES INC | Chapel Hill | E320 Health Care | $82.1M | 8 |
| GUILFORD COLLEGE | Greensboro | B420 Education | $81.9M | 16 |
| THE EDUCATIONAL FOUNDATION INC | Chapel Hill | — | $81.8M | 10 |
| METHODIST UNIVERSITY INC | Fayetteville | B03 Education | $81.6M | 15 |
| Self-Help Services Corporation | Durham | — | $80.4M | 9 |
| CAROLINA HEALTHCARE ASSOCIATES INC | Winston Salem | E21 Health Care | $79.9M | 15 |
| PIEDMONT ELECTRIC MEMBERSHIP | Hillsborough | — | $79.6M | 16 |
| EAST COAST MIGRANT HEAD START PROJECT | Raleigh | — | $79.5M | 15 |
| The Golden LEAF Inc | Rocky Mount | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $79.1M | 21 |
| BELMONT ABBEY COLLEGE INC | Belmont | B430 Education | $78.9M | 11 |
| HORIZON DEVELOPMENT PROPERTIES INC | Charlotte | L21 Housing & Shelter | $77.5M | 15 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF GREATER CHARLOTTE | Charlotte | P270 Human Services | $74.1M | 18 |
| PRESBYTERIAN HOMES INC | Greensboro | P750 Human Services | $73.5M | 8 |
| LATINO COMMUNITY CREDIT UNION | Durham | — | $73.4M | 12 |
| The Winston-Salem Foundation | Winstonsalem | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $73M | 9 |
| New Hanover Community Endowment Inc | Wilmington | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $72.9M | 3 |
| CARTERET-CRAVEN ELECTRIC COOPERATIVE | Newport | — | $72.4M | 16 |
| RANDOLPH ELECTRIC MEMBERSHIP | Asheboro | — | $72.3M | 15 |
| TELAMON CORPORATION | Raleigh | P200 Human Services | $70.8M | 7 |
| BURROUGHS WELLCOME FUND | Durham | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $70.5M | 18 |
| MURPHY MEDICAL CENTER INC | Murphy | E22 Health Care | $69.1M | 10 |
| Self-Help Ventures Fund | Durham | S310 Community Improvement & Capacity Building | $67.7M | 8 |
| UNC Rockingham Health Care Inc | Eden | E20 Health Care | $67.5M | 15 |
| CHARLOTTE COUNTRY DAY SCHOOL | Charlotte | B200 Education | $67.4M | 7 |
| ATRIUM HEALTH FOUNDATION | Charlotte | E12 Health Care | $66.4M | 9 |
| MedAssist of Mecklenburg | Charlotte | P81 Human Services | $66.3M | 3 |
| GOSHEN MEDICAL CENTER INC | Faison | E39Z Health Care | $66M | 6 |
| LATINO COMMUNITY DEVELOPMENT CENTER | Durham | P02 Human Services | $65.3M | 0 |
| NORTH CAROLINA STATE UNIVERSITY | Raleigh | B110 Education | $64.3M | 7 |
| MCNC | Research Triangle Park | U400 Science & Technology | $64.3M | 14 |
| Reynolds American Inc Trust | Winstonsalem | — | $63.9M | 1 |
| NORTH CAROLINA BAR ASSOCIATION | Cary | Y43 Mutual & Membership Benefit | $63.7M | 5 |
| SURRY-YADKIN ELECTRIC MEMBERSHIP | Dobson | — | $63.2M | 17 |
| UNC HEALTH FOUNDATION INC | Chapel Hill | B110 Education | $62.9M | 7 |
| BLUE RIDGE COMMUNITY HEALTH SERVICES INC | Hendersonville | — | $62.7M | 11 |
