| Self-Help Credit Union | Durham | — | $102.2M | 9 |
| HOSPICE & PALLIATIVE CARE CHARLOTTE REGION | Charlotte | P740 Human Services | $101.9M | 11 |
| GARDNER-WEBB UNIVERSITY | Boiling Springs | B43 Education | $101.9M | 6 |
| THE UNITED METHODIST RETIREMENT HOMES INC | Durham | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $99.2M | 9 |
| MANNA FOOD BANK INC | Mills River | K31Z Food, Agriculture & Nutrition | $98.8M | 6 |
| DAYMARK RECOVERY SERVICES INC | Salisbury | E32 Health Care | $97.8M | 10 |
| PIEDMONT HEALTH SERVICES INC | Chapel Hill | E320 Health Care | $94.9M | 10 |
| RISE AGAINST HUNGER INC | Raleigh | Q330 International, Foreign Affairs & National Security | $94.5M | 11 |
| Goodwill Industries of Northwest North Carolina Inc | Winstonsalem | J32Z Employment | $94.1M | 9 |
| GOODWILL INDUSTRIES OF THE SOUTHERN PIEDMONT INC | Charlotte | J32Z Employment | $93.5M | 11 |
| CAROLINA HEALTHCARE ASSOCIATES INC | Winston Salem | E21 Health Care | $93.3M | 14 |
| NORTH CAROLINA LIFE AND HEALTH INSURANCE GUARANTY ASSOCIATION | Raleigh | — | $91.9M | 0 |
| Queens University of Charlotte | Charlotte | B430 Education | $91.8M | 14 |
| MOSES CONE AFFILIATED PHYSICIANS INC | Greensboro | E21 Health Care | $89.3M | 11 |
| COTTON INCORPORATED | Cary | — | $89.1M | 14 |
| FRENCH BROAD ELECTRIC MEMBERSHIP CORPORATION | Marshall | — | $87.8M | 21 |
| BISSELL BALLANTYNE LEGACY FOUNDATION | Charlotte | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $86.2M | 1 |
| SECOND HARVEST FOOD BANK OF NORTHWEST NC INC | Winston Salem | K31Z Food, Agriculture & Nutrition | $85.8M | 6 |
| HORIZON DEVELOPMENT PROPERTIES INC | Charlotte | L21 Housing & Shelter | $84.4M | 14 |
| Lenoir-Rhyne University | Hickory | B420 Education | $83.7M | 12 |
| GUILFORD COLLEGE | Greensboro | B420 Education | $81.9M | 16 |
| BLUE RIDGE COMMUNITY HEALTH SERVICES INC | Hendersonville | — | $81.8M | 13 |
| MEREDITH COLLEGE | Raleigh | B430 Education | $81.8M | 11 |
| METHODIST UNIVERSITY INC | Fayetteville | B03 Education | $81.6M | 15 |
| RANDOLPH ELECTRIC MEMBERSHIP CORPORATION | Asheboro | — | $80.7M | 16 |
| Self-Help Services Corporation | Durham | — | $80.4M | 9 |
| PIEDMONT ELECTRIC MEMBERSHIP CORPORATION | Hillsborough | — | $79.6M | 16 |
| EAST COAST MIGRANT HEAD START PROJECT | Raleigh | — | $79.5M | 15 |
| The Golden LEAF Inc | Rocky Mount | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $79.1M | 21 |
| BELMONT ABBEY COLLEGE INC | Belmont | B430 Education | $78.9M | 11 |
| THE EDUCATIONAL FOUNDATION INC | Chapel Hill | — | $76.5M | 10 |
| IPAS | Chapel Hill | Q300 International, Foreign Affairs & National Security | $75.7M | 9 |
| CARTERET-CRAVEN ELECTRIC COOPERATIVE | Newport | — | $75.2M | 16 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF GREATER CHARLOTTE | Charlotte | P270 Human Services | $74.1M | 18 |
| PRESBYTERIAN HOMES INC | Greensboro | P750 Human Services | $73.5M | 8 |
| LATINO COMMUNITY CREDIT UNION | Durham | — | $73.4M | 12 |
| The Winston-Salem Foundation | Winstonsalem | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $73M | 9 |
| Coastal Horizons Center Inc | Wilmington | F210 Mental Health & Crisis Intervention | $71.3M | 8 |
| THALES ACADEMY | Raleigh | B24 Education | $71.2M | 1 |
| TELAMON CORPORATION | Raleigh | P200 Human Services | $70.8M | 7 |
| BURROUGHS WELLCOME FUND | Durham | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $70.5M | 18 |
| MURPHY MEDICAL CENTER INC DBA ERLANGER WESTERN CAROLINA HOSPITAL | Murphy | E22 Health Care | $70.2M | 12 |
| PROVIDENCE DAY SCHOOL INC | Charlotte | B200 Education | $70.2M | 13 |
| ASSOCIATED HEALTH SERVICES INC | Durham | E210 Health Care | $69M | 9 |
| MCNC | Research Triangle Park | U400 Science & Technology | $68.5M | 17 |
| Self-Help Ventures Fund | Durham | S310 Community Improvement & Capacity Building | $67.7M | 8 |
| CHARLOTTE COUNTRY DAY SCHOOL | Charlotte | B200 Education | $67.4M | 7 |
| UNC Rockingham Health Care Inc | Eden | E20 Health Care | $66.5M | 14 |
| MedAssist of Mecklenburg DBA NC Medassist | Charlotte | P81 Human Services | $66.3M | 3 |
| GOSHEN MEDICAL CENTER INC | Faison | E39Z Health Care | $66M | 6 |
