| GOODWILL OF CENTRAL AND COASTAL VIRGINIA INC | Richmond | J32 Employment | $67.2M | 8 |
| VCU Health Tappahannock Hospital | Tappahannock | E20 Health Care | $66.2M | 10 |
| THE COMMON APPLICATION INC | Arlington | B90 Education | $65.8M | 13 |
| RESOLVE TO SAVE LIVES INC | Alexandria | E70 Health Care | $65.6M | 11 |
| VIRGINIA UNION UNIVERSITY | Richmond | B430 Education | $65.6M | 8 |
| CULP FAMILY FOUNDATION | McLean | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $65.5M | 0 |
| The Concord Fund | Vienna | — | $65.1M | 3 |
| THE NATIONAL CENTER FOR MISSING AND EXPLOITED CHILDREN | Alexandria | I24Z Crime & Legal-Related | $64.5M | 10 |
| COMMUNITIES IN SCHOOLS | Arlington | B110 Education | $64.5M | 14 |
| THE COLLEGIATE SCHOOL | Richmond | B200 Education | $64.1M | 8 |
| VERSABILITY RESOURCES INC | Hampton | — | $64.1M | 12 |
| GRADUATE MANAGEMENT ADMISSION COUNCIL | Reston | B90 Education | $63.7M | 14 |
| NATIONAL APARTMENT ASSOCIATION | Arlington | — | $63.7M | 15 |
| AMERICAN INSTITUTE IN TAIWAN | Arlington | Q99Z International, Foreign Affairs & National Security | $63.6M | 10 |
| OLD DOMINION UNIVERSITY RESEARCH FOUNDATION | Norfolk | B110 Education | $63.3M | 10 |
| WOLF TRAP FOUNDATION FOR THE PERFORMING ARTS | Vienna | A600 Arts, Culture & Humanities | $62.5M | 7 |
| NATIONAL ASSOCIATION OF CONVENIENCE STORES | Alexandria | — | $61.8M | 15 |
| BAY AGING | Urbanna | P81Z Human Services | $61.6M | 6 |
| SUNNYSIDE PRESBYTERIAN HOME | Harrisonburg | P750 Human Services | $61M | 9 |
| VIRGINIA BANKERS ASSOCIATION MEDICAL AND | Glen Allen | — | $60.9M | 1 |
| CONNECTED GIVING FOUNDATION | Reston | U41 Science & Technology | $59.7M | 3 |
| Blumont Global Development Inc | Arlington | Q33 International, Foreign Affairs & National Security | $59.3M | 10 |
| ANDECO INSTITUTE | McLean | W20 Public & Societal Benefit | $59.1M | 10 |
| SOUTHERN ENVIRONMENTAL LAW CENTER | Charlottesville | — | $58.2M | 19 |
| National Milk Producers Federation | Arlington | — | $58.1M | 10 |
| The Railroad Employees National Early Retirement Major Medical Benefit Plan | Arlington | — | $57.7M | 2 |
| Young Men's Christian Association of South Hampton Roads | Chesapeake | P270 Human Services | $57.6M | 13 |
| Young Men's Christian Association of Greater Richmond (6769) | Richmond | P270 Human Services | $57.4M | 13 |
| NBA PLAYERS' HEALTH AND WELFARE BENEFIT PLAN | North Chesterfield | — | $57.3M | 0 |
| Graham Holdings Company EBAT | Arlington | — | $57.2M | 1 |
| MARY BALDWIN UNIVERSITY | Staunton | B42Z Education | $56.7M | 10 |
| LUTHERAN FAMILY SERVICES OF VIRGINIA INC DBA ENCIRCLE | Roanoke | P400 Human Services | $56.4M | 11 |
| AMERICAN ASSOCIATION OF MOTOR VEHICLE ADMINISTRATORS | Arlington | W20C Public & Societal Benefit | $56.1M | 13 |
| FOODBANK OF SOUTHEASTERN VIRGINIA | Norfolk | K310 Food, Agriculture & Nutrition | $56.1M | 4 |
| UNIVERSITY OF VIRGINIA ALUMNI ASSOCIATION | Charlottesville | B840 Education | $55.8M | 12 |
| MODZELEWSKI CHARITABLE TR US 100196 C/O STERLING FOUNDATION MANAGEMENT LLC | Reston | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $55.5M | 0 |
| WORLWIDE ASSURANCE FOR EMPLOYEES OF PUBLIC AGENCIES INC | Falls Church | Y44 Mutual & Membership Benefit | $55.4M | 9 |
| INSTITUTE FOR JUSTICE | Arlington | R600 Civil Rights, Social Action & Advocacy | $55.3M | 11 |
| THE COUNTRY CLUB OF VIRGINIA INC | Richmond | — | $54.7M | 13 |
| PRINCE GEORGE ELECTRIC COOPERATIVE | Waverly | — | $54.4M | 17 |
| EASTERN MENNONITE UNIVERSITY | Harrisonburg | — | $54.3M | 7 |
| AVERETT UNIVERSITY | Danville | B430 Education | $54.3M | 7 |
| Riverside Healthcare Association Inc | Newport News | E21 Health Care | $54.1M | 10 |
| Flint Hill School | Oakton | B200 Education | $54M | 9 |
| PBS FOUNDATION | Arlington | A113 Arts, Culture & Humanities | $53.7M | 11 |
| THE PROTESTANT EPISCOPAL HIGH SCHOOL IN VIRGINIA | Alexandria | B250 Education | $53.3M | 8 |
| BEDFORD MEMORIAL HOSPITAL | Bedford | E220 Health Care | $53.3M | 10 |
| Medical College of VA Foundation | Richmond | B11 Education | $53.2M | 7 |
| AMERICAN SOCIETY OF CIVIL ENGINEERS INC | Reston | U030 Science & Technology | $53M | 10 |
| Article IV | Arlington | W05 Public & Societal Benefit | $52.5M | 3 |
