| UNIVERSITY OF VIRGINIA LAW SCHOOL FOUNDATION | Charlottesville | B110 Education | $52.1M | 9 |
| Leadership Institute | Arlington | W700 Public & Societal Benefit | $52.1M | 13 |
| AMERICAN SOCIETY FOR TRAINING AND DEVELOPMENT | Alexandria | J030 Employment | $51.5M | 7 |
| NATIONAL RURAL LETTER CARRIERS' ASSOCIATION | Alexandria | — | $51.4M | 17 |
| AMERICAN PHYSICAL THERAPY ASSOCIATION | Alexandria | E03 Health Care | $51.3M | 29 |
| FS-ISAC INC | Reston | S41 Community Improvement & Capacity Building | $50.9M | 15 |
| ARMY NAVY COUNTRY CLUB | Arlington | — | $50.7M | 15 |
| 1693 Partners Fund | Williamsburg | B11 Education | $50.3M | 2 |
| VIRGINIA STUDENT AID FOUNDATION INC | Charlottesville | B820 Education | $50.3M | 8 |
| INTERNATIONAL WATER MANAGEMENT INSTITUTE | Alexandria | K22Z Food, Agriculture & Nutrition | $50M | 22 |
| YOUTH FOR TOMORROW-NEW LIFE CENTER INC | Bristow | O55Z Youth Development | $49.7M | 10 |
| UNITED FAITH MINISTRIES INC | Norfolk | X20 Religion-Related | $49.3M | 7 |
| JAMES MADISON UNIVERSITY FOUNDATION INC | Harrisonburg | B820 Education | $48.7M | 3 |
| THE STUDENT CONSERVATION ASSOCIATION INC | Arlington | — | $48.4M | 11 |
| WESTMINSTER-CANTERBURY OF THE BLUE RIDGE | Charlottesville | P750 Human Services | $48.3M | 8 |
| NORTHERN NECK ELECTRIC COOPERATIVE | Warsaw | — | $48.2M | 14 |
| INTERNET SOCIETY | Fredericksburg | Q300 International, Foreign Affairs & National Security | $48M | 9 |
| VIRGINIA INNOVATION PARTNERSHIP CORPORATION | Herndon | U41Z Science & Technology | $47.4M | 10 |
| AVIXA INC | Fairfax | — | $47.1M | 11 |
| NATIONAL ASSOCIATION OF CORPORATE DIRECTORS | Arlington | W03 Public & Societal Benefit | $47M | 12 |
| LAKEWOOD MANOR BAPTIST RETIREMENT COMMUNITY INC | Glen Allen | P750 Human Services | $46.9M | 12 |
| THE COLLEGE FOUNDATION OF THE UNIVERSITY OF VIRGINIA | Charlottesville | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $46.9M | 8 |
| WILLIAMSBURG LANDING INC | Williamsburg | P750 Human Services | $46.7M | 7 |
| PIEDMONT ACCESS TO HEALTH SERVICES INC | Danville | E30 Health Care | $46.6M | 11 |
| CHRISTIAN RELIEF SERVICES INC | Alexandria | Q330 International, Foreign Affairs & National Security | $46.1M | 5 |
| The Catholic Charities USA Employee Welfare Benefit Trust | Alexandria | Y20 Mutual & Membership Benefit | $46M | 0 |
| ROBIN MERGER CORPORATION INC | Arlington | B030 Education | $46M | 13 |
| EASTERN SHORE RURAL HEALTH SYSTEM INC | Onancock | E39Z Health Care | $45.8M | 14 |
| OLD DOMINION UNIVERSITY EDUCATIONAL FOUNDATION | Norfolk | B110 Education | $45.6M | 0 |
| ASSOCIATION OF THE UNITED STATES ARMY | Arlington | W030 Public & Societal Benefit | $45.5M | 11 |
| Children's Hospital and Healthcare Services Foundation | Richmond | E11 Health Care | $45.5M | 7 |
| AIR-CONDITIONING HEATING AND REFRIGERATION INSTITUTE | Arlington | S41 Community Improvement & Capacity Building | $45.1M | 13 |
| VIRGINIA PRIVATE COLLEGES BENEFITS CONSORTIUM INC | Bedford | Y43 Mutual & Membership Benefit | $45M | 2 |
| FOOD MARKETPLACE INC | Arlington | — | $44.9M | 14 |
| NEIGHBORHOOD HEALTH | Alexandria | E32 Health Care | $44.3M | 6 |
| VOANS PRESERVATION AND DEVELOPMENT HOLDING CORPORATION | Alexandria | — | $44.2M | 10 |
| DISTRIBUTIVE EDUCATION CLUBS OF AMERICA INC GROUP RETURN | Reston | B00Z Education | $44.2M | 53 |
| RANDOLPH COLLEGE INC | Lynchburg | B420 Education | $43.9M | 8 |
| THE GOOD FOOD INSTITUTE INC | Arlington | K03 Food, Agriculture & Nutrition | $43.6M | 8 |
| AMERICA'S CHARITIES | Chantilly | — | $43.6M | 6 |
| Sweet Briar Institute | Sweet Briar | B420 Education | $43.6M | 9 |
| FERRUM COLLEGE | Ferrum | B420 Education | $43.2M | 7 |
| INTERNATIONAL ASSOCIATION OF CHIEFS OF POLICE | Alexandria | — | $42.7M | 13 |
| NRA FOUNDATION INC | Fairfax | I22Z Crime & Legal-Related | $42.5M | 7 |
| Carilion Clinic | Roanoke | E192 Health Care | $42.5M | 28 |
| ASHOKA | Arlington | Q300 International, Foreign Affairs & National Security | $42.1M | 9 |
| FEEDING SOUTHWEST VIRGINIA | Salem | K31 Food, Agriculture & Nutrition | $42.1M | 6 |
| Community Residences INC | Chantilly | P73Z Human Services | $42.1M | 6 |
| MILITARY BENEFIT ASSOCIATION | Chantilly | — | $42M | 7 |
| MATTRESS RECYCLING COUNCIL INC | Alexandria | C27 Environment | $41.8M | 3 |
