| PHILA AREA INDPDNT SCHOOL BUSINESS | Wayne | Y20 Mutual & Membership Benefit | $128.3M | 2 |
| DTCare | Moon Township | J20 Employment | $127.4M | 0 |
| CONCORDIA CARE NETWORK | Cabot | G02 Diseases, Disorders & Medical Disciplines | $125.5M | 14 |
| UNITED STATES STEEL CORPORATION RETIREE INSURANCE BENEFITS TRUST | Pittsburgh | Y43 Mutual & Membership Benefit | $124.8M | 1 |
| URSINUS COLLEGE | Collegeville | B430 Education | $124.4M | 16 |
| LABORERS' DISTRICT COUNCIL OF WESTERN | Pittsburgh | — | $123.3M | 0 |
| WELLSPAN SURGERY & REHABILITATION | York | E92 Health Care | $122.5M | 15 |
| LONGWOOD GARDENS INC | Kennett Square | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $121M | 2 |
| Eastern University | St Davids | B430 Education | $120.9M | 10 |
| WAYNE MEMORIAL HOSPITAL | Honesdale | E220 Health Care | $120.9M | 9 |
| BUCKS AND MONTGOMERY COUNTY SCHOOLS | Doylestown | Y43 Mutual & Membership Benefit | $120.1M | 0 |
| HFO FOUNDATION | Philadelphia | — | $119.6M | 1 |
| LA SALLE UNIVERSITY | Philadelphia | B430 Education | $118.8M | 21 |
| HIGH FOUNDATION | Lancaster | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $118.8M | 9 |
| CHATHAM UNIVERSITY | Pittsburgh | B420 Education | $117.4M | 14 |
| AGORA CYBER CHARTER SCHOOL | King Of Prussia | B29 Education | $116.3M | 2 |
| BUSINESS LEADERSHIP ORGANIZED FOR | King Of Prussia | B82 Education | $115M | 6 |
| MOUNT NITTANY MEDICAL CENTER HEALTH | State College | E21 Health Care | $114.9M | 12 |
| PHILADELPHIA MUSEUM OF ART | Philadelphia | A510 Arts, Culture & Humanities | $114.4M | 12 |
| CARBON-SCHUYLKILL COMMUNITY HOSPITAL INC | Allentown | E21Z Health Care | $114.3M | 3 |
| PRIMARY HEALTH NETWORK | Sharon | E22Z Health Care | $114.2M | 11 |
| Marywood University | Scranton | B430 Education | $113.9M | 14 |
| PHILADELPHIA POST-ACUTE PARTNERS LLC | Philadelphia | E50 Health Care | $113M | 10 |
| NATIONAL CENTER FOR DEFENSE MANUFACTURING AND MACHINING | Johnstown | U40 Science & Technology | $112.2M | 21 |
| LEBANON VALLEY COLLEGE | Annville | B42 Education | $111.5M | 16 |
| LAW SCHOOL ADMISSION COUNCIL | Newtown | B900 Education | $110.9M | 7 |
| ALVERNIA UNIVERSITY | Reading | — | $110.6M | 18 |
| PENN STATE HEALTH HAMPDEN MEDICAL CENTER | Hershey | E22 Health Care | $110.5M | 18 |
| GAUDENZIA INC | Norristown | F200 Mental Health & Crisis Intervention | $109.7M | 13 |
| PHEAA STUDENT LOAN FOUNDATION INC | Harrisburg | W60 Public & Societal Benefit | $108.9M | 3 |
| THE WILLIAM PENN FOUNDATION | Philadelphia | — | $108.9M | 8 |
| NORTHWEST BEHAVIORAL HEALTH PARTNERSHIP | New Castle | E80 Health Care | $107.7M | 1 |
| WASHINGTON & JEFFERSON COLLEGE | Washington | B420 Education | $107.3M | 9 |
| MERCY LIFE CENTER CORPORATION | Pittsburgh | E210 Health Care | $107.2M | 10 |
| THE BANK OF NEW YORK MELLON CORPORATION | Pittsburgh | Y24 Mutual & Membership Benefit | $107.1M | 0 |
| AMERICAN SOCIETY FOR TESTING | West Conshohocken | U400 Science & Technology | $106.9M | 27 |
| ELIZABETHTOWN COLLEGE | Elizabethtown | B42Z Education | $106.3M | 14 |
| KEYSTONE RURAL HEALTH CENTER | Chambersburg | E39Z Health Care | $106M | 10 |
| SAINT FRANCIS UNIVERSITY | Loretto | B430 Education | $105.9M | 8 |
| Children's Anesthesiology Associates Ltd | Philadelphia | G91Z Diseases, Disorders & Medical Disciplines | $105.5M | 18 |
| BERKS COUNTY SCHOOL DISTRICTS HEALTH | Reading | Y20 Mutual & Membership Benefit | $104.9M | 1 |
| Greater Philadelphia Young Men's Christian Association | Conshohocken | P270 Human Services | $104.4M | 10 |
| YOUTH ADVOCATE PROGRAMS INC | Harrisburg | P300 Human Services | $103.7M | 11 |
| DR ALFRED A KUEHN INNOVATION INSTITUTE | Tarentum | W05 Public & Societal Benefit | $103.2M | 2 |
| JUNIATA COLLEGE | Huntingdon | B420 Education | $103.2M | 16 |
| CONCURRENT TECHNOLOGIES CORPORATION | Johnstown | U400 Science & Technology | $103M | 21 |
| SETON HILL UNIVERSITY | Greensburg | B420 Education | $102.6M | 10 |
| COMMUNITY CONNECTIONS FOR CHILDREN INC | York | P33B Human Services | $102.5M | 2 |
| LANSDALE HOSPITAL CORPORATION | Philadelphia | E20 Health Care | $102.1M | 8 |
| SERVICE ACCESS & MANAGEMENT INC | Reading | F300 Mental Health & Crisis Intervention | $101.5M | 8 |
