| SOOPER CREDIT UNION DBA CLIMB CREDIT UNION | Arvada | — | $50.6M | 25 |
| White River Electric Association Inc | Meeker | — | $49.4M | 15 |
| MANDARICH FAMILY FOUNDATION | Denver | Z99 Unknown | $49.2M | 0 |
| Front Range Health Partners LLC | Wheat Ridge | E99 Health Care | $49M | 11 |
| NORTH RANGE BEHAVIORAL HEALTH | Greeley | F31Z Mental Health & Crisis Intervention | $49M | 12 |
| PIPE INDUSTRY HEALTH AND WELFARE FUND OF COLORADO | Denver | — | $48.2M | 2 |
| USA Basketball | Colorado Springs | N620 Recreation & Sports | $48.1M | 10 |
| USA Swimming Inc | Colorado Springs | N67 Recreation & Sports | $47.6M | 13 |
| Towle Family Foundation | Greenwood Village | T90Z Philanthropy, Voluntarism & Grantmaking Foundations | $46.6M | 0 |
| DENVER METRO CONVENTION & VISITORS BUREAU | Denver | — | $46.5M | 10 |
| USA VOLLEYBALL | Colorado Springs | N60 Recreation & Sports | $46.5M | 17 |
| COMMUNITY DEVELOPMENT INSTITUTE HEAD START | Denver | B21 Education | $46.2M | 7 |
| KIPP Colorado Schools | Denver | B29 Education | $45.7M | 9 |
| Air Force Academy Foundation | Usaf Academy | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $45M | 5 |
| HEALTH SOLUTIONS | Pueblo | F32Z Mental Health & Crisis Intervention | $45M | 11 |
| THE WESTERN STOCK SHOW ASSOCIATION | Denver | N520 Recreation & Sports | $44.5M | 6 |
| SAN LUIS VALLEY RURAL ELECTRIC COOPERATIVE INC | Monte Vista | — | $44.3M | 19 |
| MOUNTAIN PARKS ELECTRIC INC | Granby | — | $43.7M | 21 |
| MILE HIGH UNITED WAY INC | Denver | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $43.4M | 7 |
| INTERNATIONAL DEVELOPMENT ENTERPRISES | Denver | Q023 International, Foreign Affairs & National Security | $43.4M | 10 |
| CHEYENNE MOUNTAIN ZOOLOGICAL SOCIETY | Colorado Springs | D500 Animal-Related | $42.8M | 9 |
| NURSE-FAMILY PARTNERSHIP | Denver | E80 Health Care | $42.5M | 11 |
| INSTITUTE FOR SHIPBOARD EDUCATION | Fort Collins | B400 Education | $42.5M | 7 |
| Rose Community Foundation | Denver | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $42.2M | 7 |
| HILLTOP HEALTH SERVICE CORPORATION | Grand Junction | E21Z Health Care | $42.2M | 9 |
| CLASSICAL ACADEMY | Colorado Springs | B99Z Education | $42M | 10 |
| DENVER ART MUSEUM | Denver | A510 Arts, Culture & Humanities | $42M | 8 |
| DEVELOPMENTAL DISABILITIES RESOURCE CENTER | Lakewood | P82 Human Services | $41.7M | 6 |
| DENVER BOTANIC GARDENS INC | Denver | C410 Environment | $41.6M | 7 |
| UNIVERSITY OF NORTHERN COLORADO FOUNDATION | Greeley | B110 Education | $41.6M | 2 |
| Ascent Classical Academy Charter Schools Inc | Golden | B29 Education | $41.5M | 0 |
| CATHOLIC CHARITIES & COMMUNITY SERV OF THE ARCHDIOCESE OF DENVER | Denver | P200 Human Services | $41.3M | 6 |
| MERCY HOUSING CALIFORNIA | Denver | L20 Housing & Shelter | $41.1M | 14 |
| ADOLPH COORS FOUNDATION | Denver | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $40.8M | 1 |
| P3 FOUNDATION INC | Denver | P20 Human Services | $39.6M | 3 |
| THE RESOURCE EXCHANGE INC | Colorado Springs | P200 Human Services | $39.3M | 16 |
| THE COLORADO PREVENTION CENTER | Aurora | H050 Medical Research | $39.2M | 7 |
| Arkansas Valley Regional Medical Center | La Junta | E220 Health Care | $39.2M | 8 |
| KENT DENVER COUNTRY DAY SCHOOL | Englewood | B250 Education | $39.2M | 7 |
| TEMPLE HOYNE BUELL FOUNDATION | Denver | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $39.1M | 10 |
| SOUTHEAST COLORADO POWER ASSOCIATION | La Junta | — | $39.1M | 14 |
| MUSIC ASSOCIATES OF ASPEN INC | Aspen | A6E0 Arts, Culture & Humanities | $38.6M | 22 |
| DEVELOPMENTAL DISABILITIES CENTER | Lafayette | P82Z Human Services | $38.5M | 2 |
| AMERICAN WATER WORKS ASSOCIATION | Denver | W038 Public & Societal Benefit | $38.4M | 11 |
| Grand Valley Rural Power Lines Inc | Grand Junction | — | $38.4M | 16 |
| MGMA-ACMPE | Englewood | S41 Community Improvement & Capacity Building | $38.3M | 11 |
| HOSPICE OF METRO DENVER INC | Denver | P74Z Human Services | $38.2M | 11 |
| GLOBAL GREENGRANTS FUND | Boulder | C12 Environment | $38.1M | 8 |
| Meridian Institute | Dillon | T20I Philanthropy, Voluntarism & Grantmaking Foundations | $38.1M | 13 |
| CHERRY HILLS COUNTRY CLUB | Englewood | — | $37.3M | 8 |
