| GOAL Academy | Pueblo | B29 Education | $69.1M | 9 |
| WATERSTONE SUPPORT FOUNDATION INC | Colorado Springs | T50Z Philanthropy, Voluntarism & Grantmaking Foundations | $69M | 5 |
| The New Horizons Foundation Inc | Colorado Springs | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $68.7M | 4 |
| DONOR ALLIANCE INC | Denver | E650 Health Care | $68.5M | 10 |
| CLINICA CAMPESINA FAMILY HEALTH SERVICES | Lafayette | E320 Health Care | $68.4M | 9 |
| COLORADO SCHOOL OF MINES FOUNDATION | Golden | B110 Education | $66.8M | 10 |
| DENVER CENTER FOR THE PERFORMING ARTS | Denver | A610 Arts, Culture & Humanities | $66.8M | 11 |
| AURORA COMPREHENSIVE COMMUNITY MENTAL | Aurora | F32Z Mental Health & Crisis Intervention | $66.5M | 11 |
| UNITED STATES OLYMPIC AND PARALYMPIC | Colorado Springs | N71 Recreation & Sports | $66M | 5 |
| WISCONSIN ILLINOIS SENIOR HOUSING INC | Lakewood | E91 Health Care | $65.9M | 5 |
| SUNRISE COMMUNITY HEALTH | Evans | E32Z Health Care | $65.9M | 7 |
| Care and Share Inc | Colorado Springs | K310 Food, Agriculture & Nutrition | $65.6M | 4 |
| TRU COMMUNITY CARE | Lafayette | P74Z Human Services | $65.1M | 0 |
| CREDIT UNION OF DENVER | Lakewood | — | $64.5M | 12 |
| PARKVIEW HEALTH SYSTEM INC | Pueblo | E21Z Health Care | $63.7M | 8 |
| METRO COMMUNITY PROVIDER NETWORK INC | Denver | E320 Health Care | $63.7M | 9 |
| EMPIRE ELECTRIC ASSOCIATION INC | Cortez | — | $63M | 16 |
| HIGHLINE ELECTRIC ASSOCIATION | Holyoke | — | $61.2M | 18 |
| San Isabel Electric Association Inc | Pueblo | — | $61M | 14 |
| DIVERSUS HEALTH INC GROUP RETURN | Colorado Springs | E210 Health Care | $60.7M | 11 |
| Valley-Wide Health Systems Inc | Alamosa | E80Z Health Care | $60.6M | 7 |
| COLORADO HEALTH BENEFIT EXCHANGE | Denver | E80 Health Care | $60.3M | 8 |
| DENVER ZOOLOGICAL FOUNDATION | Denver | D115 Animal-Related | $60.1M | 15 |
| ALLIANCE FOR CHOICE IN EDUCATION | Greenwood Village | B82 Education | $59.5M | 9 |
| HopeWest | Grand Junction | M99Z Public Safety, Disaster Preparedness & Relief | $59.1M | 12 |
| USA HOCKEY INC | Colorado Springs | N80 Recreation & Sports | $58.9M | 9 |
| THE ANSCHUTZ FOUNDATION | Denver | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $58M | 8 |
| JUNIOR ACHIEVEMENT USA | Colorado Springs | O53 Youth Development | $57.6M | 16 |
| CONSERVATION LEGACY | Durango | O51 Youth Development | $57.4M | 8 |
| Colorado Museum of Natural History | Denver | A560 Arts, Culture & Humanities | $57.2M | 10 |
| Bethesda Foundation | Colorado Springs | P750 Human Services | $57.2M | 16 |
| ROCKY MOUNTAIN PLANNED PARENTHOOD INC | Denver | E420 Health Care | $56.4M | 12 |
| COLORADO CATTLEMEN'S AGRICULTURAL | Lakewood | K250 Food, Agriculture & Nutrition | $56.3M | 1 |
| NATIONAL CATTLEMEN'S BEEF ASSOCIATION | Centennial | S41 Community Improvement & Capacity Building | $55.9M | 10 |
| AMERICAN INDIAN COLLEGE FUND | Denver | B110 Education | $55.5M | 8 |
| Ahimsa Foundation | Niwot | D20 Animal-Related | $55.1M | 0 |
| COLORADO ACADEMY | Denver | B24Z Education | $54.3M | 7 |
| FRASIER MEADOWS MANOR INC | Boulder | P750 Human Services | $52.7M | 11 |
| CARING FOR DENVER FOUNDATION | Denver | F20 Mental Health & Crisis Intervention | $52.7M | 10 |
| Denver Rescue Mission | Denver | P280 Human Services | $51.8M | 11 |
| REISHER FAMILY FOUNDATION | Lakewood | B82 Education | $51.4M | 4 |
| White River Electric Association Inc | Meeker | — | $51.2M | 14 |
| UNITED STATES MEAT EXPORT | Denver | — | $50.6M | 6 |
| PUEBLO COMMUNITY HEALTH CENTER INC | Pueblo | — | $50.6M | 11 |
| SOUTHWEST COLORADO MENTAL HEALTH CENTER | Durango | F32Z Mental Health & Crisis Intervention | $49.9M | 10 |
| MANDARICH FAMILY FOUNDATION | Denver | Z99 Unknown | $49.2M | 0 |
| Front Range Health Partners LLC | Wheat Ridge | E99 Health Care | $49M | 11 |
| USA Basketball | Colorado Springs | N620 Recreation & Sports | $48.1M | 10 |
| TRINIDAD AREA HEALTH ASSOCIATION | Trinidad | E20Z Health Care | $48.1M | 10 |
| DENVER METRO CONVENTION & VISITORS | Denver | — | $47.1M | 8 |
