| COLORADO STATE UNIVERSITY RESEARCH FOUNDATION | Fort Collins | K26E Food, Agriculture & Nutrition | $23.3M | 11 |
| BOYS AND GIRLS CLUBS OF METRO DENVER INC | Denver | — | $23.3M | 8 |
| TRAILHEAD INSTITUTE | Denver | E60Z Health Care | $23.2M | 10 |
| COMMUNITY DEVELOPMENT INSTITUTE KANSAS | Denver | — | $23.2M | 7 |
| PHOENIX MULTISPORT INC | Denver | F21 Mental Health & Crisis Intervention | $23.1M | 10 |
| COMPASSION & CHOICES | Littleton | R67 Civil Rights, Social Action & Advocacy | $22.8M | 6 |
| COMMUNITY HOUSING CONCEPTS INC | Denver | L21 Housing & Shelter | $22.8M | 0 |
| JEWISHColorado | Denver | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $22.8M | 7 |
| LA PUENTE HOME INC | Alamosa | — | $22.7M | 1 |
| Stargate Charter School | Thornton | B29 Education | $22.7M | 10 |
| REISHER FAMILY FOUNDATION | Lakewood | B82 Education | $22.6M | 4 |
| DENVER COMMUNITY CREDIT UNION DBA ZING CREDIT UNION | Denver | — | $22.6M | 10 |
| MERCY HOUSING MANAGEMENT GROUP | Denver | P85B Human Services | $22.6M | 11 |
| MERCY HOUSING NORTHWEST | Denver | — | $22.6M | 8 |
| THE CATHOLIC FOUNDATION NO COLORADO | Greenwood Village | X120 Religion-Related | $22.4M | 4 |
| THIRD WAY CENTER INC | Denver | F60Z Mental Health & Crisis Intervention | $22M | 9 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF BOULDER VALLEY | Lafayette | — | $21.9M | 7 |
| COLORADO SPRINGS HEALTH FOUNDATION | Colorado Springs | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $21.8M | 2 |
| JEWISH FAMILY SERVICE OF COLORADO INC | Denver | P40Z Human Services | $21.8M | 11 |
| Operating Engineers HW Fund for Colorado Trust Fund for Colorado | Arvada | — | $21.8M | 0 |
| Charis Bible College Inc | Woodland Park | X11 Religion-Related | $21.7M | 4 |
| THURSTON FOUNDATION | Littleton | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $21.7M | 0 |
| HUMANE SOCIETY OF THE PIKES PEAK REGION | Colorado Springs | D200 Animal-Related | $21.5M | 10 |
| FOOTHILLS GATEWAY INC | Fort Collins | P820 Human Services | $21.5M | 7 |
| Banning Lewis Ranch Academy | Colorado Springs | B29 Education | $21.4M | 0 |
| FLYING CROWN LAND GROUP | Steamboat Springs | W99 Public & Societal Benefit | $21.4M | 5 |
| CROSSROADS' TURNING POINTS INC | Pueblo | F220 Mental Health & Crisis Intervention | $21.3M | 3 |
| THE COLORADO NONPROFIT DEVELOPMENT CENTER | Denver | S022 Community Improvement & Capacity Building | $21.2M | 7 |
| MOUNTAIN STATES EMPLOYERS COUNCIL INC | Denver | — | $21.1M | 10 |
| MARILLAC CLINIC INC | Grand Junction | E32 Health Care | $21M | 10 |
| KOSON Schools dba STEM School Highlands Ranch | Highlands Ranch | B29 Education | $21M | 0 |
| Liberty Common School | Fort Collins | B29 Education | $20.9M | 9 |
| GEORGE W CLAYTON TRUST | Denver | P300 Human Services | $20.9M | 8 |
| ASPEN COMMUNITY FOUNDATION | Basalt | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $20.9M | 4 |
| SCRUM ALLIANCE INC | Westminster | S41 Community Improvement & Capacity Building | $20.9M | 18 |
| SHALOM PARK | Aurora | P750 Human Services | $20.8M | 7 |
| ILLUMINATIVE INC | Boulder | A12 Arts, Culture & Humanities | $20.8M | 10 |
| WESTERN INTERSTATE COMMISSION FOR HIGHER EDUCATION | Boulder | — | $20.7M | 8 |
| CHEF ANN FOUNDATION | Boulder | B990 Education | $20.5M | 1 |
| WESTERN COLORADO COMMUNITY FOUNDATION INC | Grand Junction | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $20.5M | 2 |
| Cheyenne Mountain Charter Academy | Colorado Springs | B20Z Education | $20.3M | 2 |
| Colorado Lawyer Trust Account Foundation | Denver | I42I Crime & Legal-Related | $20.2M | 1 |
| NATIONAL ENVIRONMENTAL HEALTH ASSOCIATION | Denver | — | $20M | 7 |
| SUMMIT COMMUNITY CARE CLINIC INC | Frisco | E32 Health Care | $20M | 12 |
| HIGHLINE ACADEMY | Denver | B24 Education | $19.9M | 5 |
| THE CONSOLIDATED MUTUAL WATER COMPANY | Lakewood | — | $19.9M | 15 |
| SUNNY VISTA LIVING CENTER | Colorado Springs | E91Z Health Care | $19.9M | 6 |
| Professional Rodeo Cowboys Association | Colorado Springs | — | $19.8M | 10 |
| RED ROCKS CREDIT UNION | Littleton | — | $19.7M | 8 |
| COLORADO SHEET METAL WORKERS' LOCAL 9 HEALTH FUND | Denver | E80 Health Care | $19.7M | 4 |
