| MASSACHUSETTS SOCIETY FOR THE PREVENTION | Boston | D200 Animal-Related | $144.8M | 12 |
| CENTER FOR HUMAN DEVELOPMENT INC | Springfield | P46 Human Services | $142.1M | 7 |
| TRUMP VANCE INAUGURAL COMMITTEE INC | Beverly | — | $141.8M | 0 |
| BAYSTATE FRANKLIN MEDICAL CENTER | Greenfield | E220 Health Care | $141.1M | 17 |
| FACULTY PRACTICE FOUNDATION | Boston | — | $138.3M | 0 |
| MASSACHUSETTS MEDICAL SOCIETY | Waltham | — | $138M | 17 |
| Dana-FarberMass General Brigham Cancer Care INC | Boston | H300 Medical Research | $137.6M | 12 |
| PRESERVATION OF AFFORDABLE HOUSING INC | Boston | L250 Housing & Shelter | $136.7M | 18 |
| FOUNDATION TO COMBAT ANTI-SEMITISM INC | Foxborough | R12 Civil Rights, Social Action & Advocacy | $135.4M | 2 |
| PEOPLE ACTING IN COMMUNITY ENDEAVORS INC | New Bedford | — | $135.2M | 2 |
| AGESPAN INC | Lawrence | P81Z Human Services | $133.8M | 7 |
| THE NEW ENGLAND CENTER FOR CHILDREN INC | Southborough | — | $129.2M | 9 |
| Harvard Management Company Inc | Boston | B020 Education | $128.7M | 12 |
| ASSUMPTION UNIVERSITY | Worcester | B420 Education | $127.1M | 15 |
| GPU COMPANIES HEALTH CARE PLAN FOR NON- | Heritage Dr N Qu | Y43 Mutual & Membership Benefit | $126.2M | 0 |
| SOUTH SHORE HEALTH SYSTEM INC | South Weymouth | E110 Health Care | $124.4M | 6 |
| SPECTRUM HEALTH SYSTEMS INC | Westborough | F30 Mental Health & Crisis Intervention | $123.6M | 14 |
| RIVERVIEW SCHOOL INC | East Sandwich | B280 Education | $122M | 5 |
| EMMANUEL COLLEGE | Boston | B430 Education | $120.9M | 8 |
| MASSACHUSETTS LIFE & HEALTH INSURANCE | Springfield | Y20Z Mutual & Membership Benefit | $120.7M | 1 |
| DANA-FARBER INC | Boston | Z99 Unknown | $120.5M | 3 |
| WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH | Cambridge | H92 Medical Research | $120.2M | 12 |
| SPRINGWELL INC | Waltham | P810 Human Services | $119.5M | 9 |
| OPEN SKY COMMUNITY SERVICES INC | Worcester | P80Z Human Services | $119.3M | 8 |
| JUDGE ROTENBERG EDUCATIONAL CENTER INC | Canton | B280 Education | $118.6M | 7 |
| MILFORD REGIONAL PHYSICIAN GROUP INC | Milford | E110 Health Care | $117.8M | 7 |
| LESLEY UNIVERSITY | Cambridge | — | $117.7M | 16 |
| BOSTON SYMPHONY ORCHESTRA INC | Boston | A690 Arts, Culture & Humanities | $117.1M | 11 |
| National Collegiate Investors & | Hadley | B12 Education | $116.6M | 8 |
| MEDICAL CARE OF BOSTON MANAGEMENT CORP | Charlestown | E21 Health Care | $116.6M | 19 |
| HARBOR HEALTH SERVICES INC | Mattapan | E320 Health Care | $115.6M | 12 |
| NATIONAL FIRE PROTECTION ASSOCIATION | Quincy | M030 Public Safety, Disaster Preparedness & Relief | $115.2M | 10 |
| UNIVERSITY OF MASSACHUSETTS FOUNDATION | Newton | B110 Education | $113.4M | 10 |
| Massachusetts Mutual Employee Health Benefit Trust | Springfield | — | $113.4M | 0 |
| WAY FINDERS INC | Springfield | L210 Housing & Shelter | $113.1M | 8 |
| MASSACHUSETTS HIGHER EDUCATION | Boston | — | $113.1M | 29 |
| FENWAY COMMUNITY HEALTH CENTER INC | Boston | E300 Health Care | $112.6M | 9 |
| RIVERSIDE COMMUNITY CARE INC | Dedham | E20Z Health Care | $111.9M | 11 |
| BROCKTON AREA MULTI-SERVICES INC | Brockton | P73Z Human Services | $111.6M | 11 |
| CURRY COLLEGE | Milton | B420 Education | $110.2M | 10 |
| ELEMENT CARE INC | Lynn | P81Z Human Services | $110M | 11 |
| COMMUNITY CARE COOPERATIVE INC | Boston | E31 Health Care | $109.7M | 7 |
| CORE PHYSICIANS LLC | Charlestown | E30 Health Care | $109.3M | 24 |
| NORTHFIELD MOUNT HERMON SCHOOL | Mount Hermon | B250 Education | $107.3M | 11 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION | Boston | P270 Human Services | $106.5M | 9 |
| JEANNE D'ARC CREDIT UNION | Lowell | — | $106.2M | 19 |
| Physicians Organization at Children's | Boston | E83Z Health Care | $104.9M | 31 |
| LYNN COMMUNITY HEALTH INC | Lynn | — | $104.3M | 12 |
| HARVARD PRIVATE CAPITAL REALTY INC | Boston | B110 Education | $103.8M | 3 |
| BROOKSBY VILLAGE INC | Peabody | L22 Housing & Shelter | $103.2M | 8 |
