| Otterbein University | Westerville | B430 Education | $103.7M | 12 |
| OTTERBEIN HOMES | Lebanon | P750 Human Services | $102.2M | 8 |
| SEVEN SEVENTEEN CREDIT UNION INC | Warren | — | $99.5M | 23 |
| HOSPICE OF DAYTON INC | Dayton | P740 Human Services | $98M | 10 |
| University of Mount Union | Alliance | B420 Education | $97.4M | 13 |
| AREA AGENCY ON AGING DISTRICT 7 INC | Jackson | — | $97.1M | 4 |
| AVITA HEALTH SYSTEM | Galion | E21 Health Care | $94.8M | 8 |
| FRANKLIN UNIVERSITY | Columbus | — | $94.7M | 14 |
| FREESTORE FOODBANK INC | Cincinnati | K31Z Food, Agriculture & Nutrition | $94.2M | 9 |
| MEMORIAL HOSPITAL | Toledo | E220 Health Care | $93.9M | 16 |
| MIDDLETOWN WORKS HOURLY & SALARIED UNION RETIREES HEALTH CARE FUND | Middletown | Y43 Mutual & Membership Benefit | $92.2M | 5 |
| OhioGuidestone | Berea | F33Z Mental Health & Crisis Intervention | $91.4M | 10 |
| SELBY GENERAL HOSPITAL | Marietta | E220 Health Care | $89.9M | 10 |
| Hospital Properties Inc | Columbus | — | $89.1M | 6 |
| COLUMBUS ZOOLOGICAL PARK ASSOCIATION | Powell | D500 Animal-Related | $88.8M | 10 |
| Wayne Hospital Company | Greenville | E220 Health Care | $88.8M | 9 |
| THE MILLIRON FOUNDATION | Mansfield | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $88.5M | 0 |
| AMERICAN FINANCIAL GROUP VOLUNTARY EMPLOYEES BENEFICIARY TRUST | Cincinnati | — | $88.1M | 0 |
| UNITED CHURCH INSURANCE ASSOCIATION | Cleveland | Y200 Mutual & Membership Benefit | $87.8M | 5 |
| Hospice of The Western Reserve Inc | Cleveland | P740 Human Services | $87.8M | 8 |
| PROMEDICA CONTINUUM SERVICES | Toledo | E21 Health Care | $87.6M | 7 |
| Bon Secours Mercy Health Foundation | Blue Ash | X12 Religion-Related | $87M | 9 |
| MENORAH PARK CENTER FOR SENIOR LIVING | Beachwood | P750 Human Services | $86.4M | 8 |
| COALITION FOR THE COMMON GOOD | Yellow Springs | B420 Education | $85.8M | 15 |
| AULTMAN HEALTH FOUNDATION | Canton | E21 Health Care | $85M | 29 |
| CITY HOSPITAL ASSOCIATION | East Liverpool | E220 Health Care | $84.8M | 12 |
| 64 TELHIO CREDIT UNION INC | Columbus | — | $84.7M | 13 |
| TALBERT HOUSE | Cincinnati | P200 Human Services | $84.4M | 15 |
| PIONEER RURAL ELECTRIC COOPERATIVE INC | Piqua | — | $83.5M | 18 |
| Playhouse Square Foundation | Cleveland | A610 Arts, Culture & Humanities | $83.1M | 8 |
| Superior Credit Union Inc | Lima | A30 Arts, Culture & Humanities | $83.1M | 13 |
| 42 KEMBA CREDIT UNION INC | West Chester | — | $82.3M | 10 |
| GATEWAY ECONOMIC DEVELOPMENT CORPORATION OF GREATER CLEVELAND | Cleveland | S310 Community Improvement & Capacity Building | $82.3M | 2 |
| PARAMOUNT ADVANTAGE | Toledo | — | $82.1M | 7 |
| BREAD FINANCIAL PAYMENTS INC EMPLOYEE BENEFITS TRUST | Columbus | — | $81.6M | 1 |
| THE BOARD OF DIRECTORS OF WITTENBERG COLLEGE | Springfield | B420 Education | $81.3M | 7 |
| Cleveland Bakers and Teamsters Health and Welfare Fund | Valley View | — | $80.7M | 2 |
| COMMUNITY HEALTH CENTER OF BRANCH COUNTY | Toledo | E21 Health Care | $80.5M | 14 |
| AREA AGENCY ON AGING REGION 9 INC | Cambridge | P81Z Human Services | $80.5M | 9 |
| DIRECTION HOME AKRON CANTON AREA AGENCY ON AGING | Uniontown | P81Z Human Services | $80M | 9 |
| The Musical Arts Association | Cleveland | A690 Arts, Culture & Humanities | $79.4M | 8 |
| HATTIE LARLHAM CENTER FOR CHILDREN WITH DISABILITIES | Mantua | P82Z Human Services | $79.2M | 9 |
| IRONWORKERS DISTRICT COUNCIL OF SOUTHERN OHIO AND VICINITY BENEFIT TRUST | Vandalia | — | $78.7M | 1 |
| Equitas Health Inc | Columbus | G81Z Diseases, Disorders & Medical Disciplines | $78.6M | 12 |
| MUSIC AND EVENT MANAGEMENT INC | Cincinnati | A200 Arts, Culture & Humanities | $76.7M | 10 |
| HEALTH PARTNERS OF WESTERN OHIO | Lima | E32 Health Care | $76.7M | 12 |
| HOPEWELL HEALTH CENTERS INC | Chillicothe | — | $76.6M | 11 |
| OHIO HARNESS HORSEMEN'S ASSOCIATION AS AGENT FOR BENEFICIARIES | Grove City | — | $75.6M | 1 |
| ORIANA HOUSE INC | Akron | I440 Crime & Legal-Related | $74.7M | 15 |
| Tiffin University | Tiffin | B430 Education | $74.7M | 14 |
