| John Carroll University | University Heights | B430 Education | $171.1M | 16 |
| Christian Aid Ministries | Berlin | X200 Religion-Related | $168.7M | 20 |
| ALLIANCE PHYSICIANS INC | Miamisburg | E300 Health Care | $168.5M | 20 |
| The College of Wooster | Wooster | B420 Education | $165.8M | 17 |
| FISHER-TITUS MEDICAL CENTER | Norwalk | E22 Health Care | $165.5M | 10 |
| GOSPEL LIGHT MENNONITE CHURCH MEDICAL AID PLAN INC | Canton | X20 Religion-Related | $163.4M | 12 |
| BALDWIN WALLACE UNIVERSITY | Berea | B430 Education | $162.9M | 14 |
| JACK JOSEPH AND MORTON MANDEL SUPPORTING FOUNDATION | Cleveland | T110 Philanthropy, Voluntarism & Grantmaking Foundations | $161.6M | 7 |
| OHIO CARPENTERS' HEALTH FUND | Columbus | Y43 Mutual & Membership Benefit | $158.4M | 7 |
| EMMA L BIXBY MEDICAL CENTER | Toledo | E220 Health Care | $155.8M | 15 |
| FRANCISCAN UNIVERSITY OF STEUBENVILLE | Steubenville | B430 Education | $155.4M | 15 |
| I AM BOUNDLESS INC | Worthington | P82 Human Services | $154.3M | 10 |
| Ohio Northern University | Ada | B420 Education | $154.2M | 10 |
| THE PROCTER & GAMBLE RETIREE BENEFIT TRUST | Cincinnati | — | $153.3M | 0 |
| 37 KEMBA FINANCIAL CREDIT UNION | Gahanna | — | $152.9M | 20 |
| The University of Findlay | Findlay | B420 Education | $152.5M | 11 |
| ONEOHIO RECOVERY FOUNDATION INC | Westerville | F20 Mental Health & Crisis Intervention | $148.3M | 2 |
| WOOD COUNTY HOSPITAL ASSOCIATION | Bowling Green | E220 Health Care | $146.1M | 9 |
| BATTELLE VOLUNTARY EMPLOYEES' BENEFICIARY ASSOCIATION | Columbus | — | $143.6M | 4 |
| FULTON COUNTY HEALTH CENTER | Wauseon | E220 Health Care | $141.8M | 11 |
| OHIO VIRTUAL ACADEMY | Maumee | B200 Education | $140.2M | 7 |
| MARY RUTAN HOSPITAL | Bellefontaine | E220 Health Care | $139.6M | 12 |
| OHIO LIVING COMMUNITIES | Westerville | P750 Human Services | $139.4M | 12 |
| JEWISH FEDERATION OF CLEVELAND | Cleveland | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $138.7M | 15 |
| BAY PARK COMMUNITY HOSPITAL | Toledo | E220 Health Care | $135.4M | 13 |
| Mid-Ohio Foodbank | Grove City | — | $133.6M | 8 |
| ASHLAND UNIVERSITY | Ashland | B430 Education | $132.3M | 11 |
| Honda Health and Welfare Benefits Trust | Marysville | — | $132.3M | 0 |
| HOLZER HEALTH SYSTEM | Gallipolis | E210 Health Care | $131.1M | 31 |
| Cleveland Museum of Art | Cleveland | A510 Arts, Culture & Humanities | $129.1M | 15 |
| Council on Aging of Southwestern Ohio | Blue Ash | P81Z Human Services | $129M | 11 |
| THE SHELBY COUNTY MEMORIAL HOSPITAL ASSOCIATION | Sidney | E220 Health Care | $128.7M | 10 |
| UNITED FOOD & COMMERCIAL WORKERS UNION LOCAL 880 - EMPLOYER | Broadview Heights | — | $127.8M | 0 |
| WESTERN RESERVE AREA AGENCY ON AGING | Cleveland | P810 Human Services | $127.3M | 19 |
| COMPUNET CLINICAL LABORATORIES LLC | Dayton | — | $125.5M | 12 |
| THE WEXNER FAMILY CHARITABLE FUND | New Albany | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $122.8M | 0 |
| CAPITAL UNIVERSITY | Columbus | B430 Education | $122.6M | 12 |
| OHIO WESLEYAN UNIVERSITY | Delaware | B420 Education | $122.6M | 12 |
| SALEM COMMUNITY HOSPITAL | Salem | E220 Health Care | $116.7M | 13 |
| GREATER CLEVELAND FOOD BANK INC | Cleveland | K31Z Food, Agriculture & Nutrition | $116.5M | 13 |
| NATIONAL CHURCH RESIDENCES | Columbus | L210 Housing & Shelter | $116.1M | 17 |
| UNIVERSITY OF TOLEDO PHYSICIANS CLINICAL FACULTY INC | Toledo | — | $115.5M | 42 |
| First Catholic Slovak Union of the United States of America and Canada | Independence | — | $114.4M | 20 |
| THE ELIZABETH GAMBLE DEACONESS HOME ASSOCIATION | Cincinnati | E12 Health Care | $110M | 2 |
| UNITED CHURCH HOMES INC | Marion | P750 Human Services | $109.2M | 11 |
| SIGNATURE HEALTH INC | Kirtland | F30 Mental Health & Crisis Intervention | $109M | 12 |
| GOODWILL EASTER SEALS MIAMI VALLEY | Dayton | J32Z Employment | $108.4M | 10 |
| JOINT TOWNSHIP DISTRICT MEMORIAL HOSPITAL | St Marys | E220 Health Care | $107.7M | 14 |
| THE PROCTER & GAMBLE BENEFIT PLAN TRUST | Cincinnati | — | $106.4M | 0 |
| DEFIANCE HOSPITAL INC | Toledo | E220 Health Care | $103.9M | 14 |
