| National Board of Medical Examiners | Philadelphia | E030 Health Care | $174.2M | 30 |
| UPMC Carlisle | Harrisburg | E220 Health Care | $174.1M | 15 |
| LINCOLN BENEFIT TRUST | New Oxford | — | $174.1M | 1 |
| MERAKEY ALLEGHENY VALLEY SCHOOL | Lafayette Hill | P730 Human Services | $173.4M | 10 |
| PHILADELPHIA COLLEGE OF OSTEOPATHIC | Philadelphia | B500 Education | $173.2M | 15 |
| Children's Surgical Associates Ltd | Philadelphia | E31Z Health Care | $173.2M | 11 |
| SUMMIT PHYSICIAN SERVICES | York | — | $172.6M | 21 |
| MERAKEY PENNSYLVANIA | Lafayette Hill | F20 Mental Health & Crisis Intervention | $170.6M | 12 |
| BROTHER'S BROTHER FOUNDATION | Pittsburgh | Q300 International, Foreign Affairs & National Security | $168.5M | 4 |
| ROBERT MORRIS UNIVERSITY | Moon Township | — | $168.4M | 18 |
| ARIA HEALTH PHYSICIAN SERVICES | Philadelphia | E32Z Health Care | $167.6M | 5 |
| GANNON UNIVERSITY | Erie | B430 Education | $166M | 14 |
| ARCADIA UNIVERSITY | Glenside | B430 Education | $164.4M | 17 |
| LANCASTER GENERAL MEDICAL GROUP | East Petersburg | E31Z Health Care | $163.6M | 19 |
| PHILADELPHIA UNIVERSITY | Philadelphia | B430 Education | $163.5M | 7 |
| NAZARETH HOSPITAL | Newtown Square | E220 Health Care | $161.7M | 14 |
| LEHIGH VALLEY HOSPITAL-HAZLETON | Allentown | E220 Health Care | $160M | 13 |
| MELMARK INC | Berwyn | P700 Human Services | $156.8M | 14 |
| SUSQUEHANNA UNIVERSITY | Selinsgrove | B420 Education | $155.8M | 12 |
| WILKES UNIVERSITY | Wilkes Barre | B110 Education | $151.9M | 15 |
| WOODS SERVICES INC | Langhorne | P820 Human Services | $151.7M | 13 |
| ST LUKE'S QUAKERTOWN HOSPITAL | Allentown | E22Z Health Care | $150.3M | 5 |
| MUHLENBERG COLLEGE | Allentown | B420 Education | $150.2M | 12 |
| RETIREES OF THE GOODYEAR TIRE & RUBBER | Pittsburgh | Y43 Mutual & Membership Benefit | $150.1M | 9 |
| WILLOW VALLEY COMMUNITIES | Lancaster | P750 Human Services | $149M | 12 |
| LEHIGH VALLEY HOSPITAL - SCHUYLKILL | Allentown | E220 Health Care | $147.5M | 10 |
| MERCYHURST UNIVERSITY | Erie | B420 Education | $146.9M | 12 |
| Duke Energy Corp Welfare Benefits Trust | Pittsburgh | — | $146.1M | 1 |
| LAW ENFORCEMENT HEALTH BENEFITSINC | Philadelphia | — | $146M | 14 |
| MESSIAH UNIVERSITY | Mechanicsburg | B420 Education | $144.4M | 11 |
| THE GUTHRIE CLINIC | Sayre | E21 Health Care | $143.8M | 19 |
| ALLEGHENY COLLEGE | Meadville | B420 Education | $141.2M | 11 |
| YORK COLLEGE OF PENNSYLVANIA | York | B430 Education | $140.6M | 13 |
| GREATER PENNSYLVANIA CARPENTERS | Pittsburgh | — | $139.7M | 0 |
| KENCREST SERVICES | Blue Bell | P820 Human Services | $138.3M | 11 |
| BUTLER MEDICAL PROVIDERS | Butler | E31Z Health Care | $137.7M | 9 |
| American Association for Cancer Research Inc | Philadelphia | H300 Medical Research | $137.4M | 9 |
| EASTERN ATLANTIC STATES REGIONAL | Pittsburgh | — | $137.2M | 48 |
| REACH CYBER CHARTER SCHOOL | Harrisburg | B28 Education | $135.5M | 8 |
| ARMSTRONG COUNTY MEMORIAL HOSPITAL | Kittanning | E220 Health Care | $135.2M | 11 |
| GREATER DELAWARE VALLEY SOCIETY OF | Philadelphia | H9B Medical Research | $134.5M | 9 |
| KENNEDY MEDICAL GROUP PRACTICE PC | Philadelphia | E20 Health Care | $133.7M | 6 |
| CENTRAL PENNSYLVANIA FOOD BANK | Harrisburg | K31Z Food, Agriculture & Nutrition | $132.6M | 5 |
| MORAVIAN UNIVERSITY | Bethlehem | — | $132.1M | 15 |
| EMPLOYEE BENEFIT TR OF EASTERN PA | Easton | — | $131.8M | 0 |
| THE ANNENBERG FOUNDATION | Conshohocken | B82 Education | $131.6M | 5 |
| CENTRAL PENNSYLVANIA TEAMSTERS HEALTH | Wyomissing | — | $131.6M | 11 |
| POINT PARK UNIVERSITY | Pittsburgh | — | $131.3M | 18 |
| UPMC Lititz | Harrisburg | E220 Health Care | $130.3M | 15 |
| DESALES UNIVERSITY | Center Valley | B430 Education | $129.5M | 16 |
