| PALMER COLLEGE FOUNDATION | Davenport | B500 Education | $93.6M | 18 |
| IMPACTLIFE | Davenport | — | $91.4M | 8 |
| DORDT UNIVERSITY | Sioux Center | B420 Education | $90.2M | 7 |
| COLLINS COMMUNITY CREDIT UNION | Cedar Rapids | — | $89.1M | 11 |
| CORNELL COLLEGE | Mount Vernon | B420 Education | $87.9M | 7 |
| UNITYPOINT HEALTH-MARSHALLTOWN | Marshalltown | E22 Health Care | $86.7M | 13 |
| COE COLLEGE | Cedar Rapids | B42 Education | $80M | 12 |
| Sioux Center Health | Sioux Center | — | $77M | 7 |
| Principal Trust-Health&Disability Ben for Act & Non-Medicare Elig Rets | Des Moines | — | $76.4M | 0 |
| IOWA LAKES ELECTRIC COOPERATIVE | Estherville | W80 Public & Societal Benefit | $74.7M | 24 |
| SIOUXLAND REGIONAL CANCER CENTER | Sioux City | G30Z Diseases, Disorders & Medical Disciplines | $73.2M | 7 |
| JOHN AND MARY PAPPAJOHN FAMILY | Des Moines | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $72.2M | 2 |
| COOPERATIVE WELFARE BEN FD (IA & NE) | West Des Moines | — | $71.2M | 0 |
| LINN COUNTY RURAL ELECTRIC COOPERATIVE | Marion | — | $69.4M | 15 |
| COMMUNITY 1ST CREDIT UNION | Ottumwa | — | $68.6M | 10 |
| EASTERN IOWA LIGHT & POWER COOPERATIVE | Wilton | — | $68.3M | 24 |
| UNITY HEALTHCARE | Muscatine | E220 Health Care | $68M | 15 |
| MORNINGSIDE UNIVERSITY | Sioux City | B420 Education | $67.6M | 9 |
| NORTHWESTERN COLLEGE | Orange City | B420 Education | $67.2M | 7 |
| Simpson College | Indianola | B420 Education | $66.7M | 12 |
| HENRY COUNTY HEALTH CENTER INC | Mt Pleasant | E20 Health Care | $65.5M | 4 |
| GRINNELL REGIONAL MEDICAL CENTER | Grinnell | E220 Health Care | $64.4M | 11 |
| LORAS COLLEGE | Dubuque | — | $63.3M | 10 |
| GRAND VIEW UNIVERSITY | Des Moines | B420 Education | $63.1M | 15 |
| PRIMARY HEALTH CARE INC | Des Moines | E32Z Health Care | $62.4M | 13 |
| NORTH WEST RURAL ELECTRIC COOPERATIVE | Orange City | — | $61.5M | 18 |
| Buena Vista University | Storm Lake | B420 Education | $60.3M | 13 |
| MIDLAND POWER COOPERATIVE | Boone | — | $60.1M | 21 |
| SHENANDOAH MEDICAL CENTER | Shenandoah | E22Z Health Care | $59.7M | 8 |
| ST LUKE'SJONES REGIONAL MEDICAL CENTER | Anamosa | B20 Education | $56.7M | 11 |
| HAWKEYE AREA COMMUNITY ACTION | Hiawatha | P200 Human Services | $56.6M | 4 |
| Imagine the Possibilities Inc | Maquoketa | — | $56.2M | 7 |
| SIOUX VALLEY MEMORIAL HOSPITAL | Cherokee | E22Z Health Care | $55.6M | 7 |
| MAHARISHI INTERNATIONAL UNIVERSITY | Fairfield | B430 Education | $55.3M | 14 |
| UNIVERSITY OF NORTHERN IOWA | Cedar Falls | B110 Education | $54.3M | 8 |
| IOWA SOYBEAN ASSOCIATION | Ankeny | — | $53.8M | 22 |
| DUTRAC COMMUNITY CREDIT UNION | Dubuque | W61 Public & Societal Benefit | $53.1M | 19 |
| MOUNT MERCY UNIVERSITY | Cedar Rapids | B420 Education | $53.1M | 9 |
| Wells Fargo & Co Employee Benefit Trust for Retiree | Des Moines | — | $52.6M | 0 |
| Lifeserve Blood Center | Johnston | E61 Health Care | $52.4M | 8 |
| CENTRAL COLLEGE | Pella | B420 Education | $51.7M | 8 |
| WILLIAM PENN UNIVERSITY | Oskaloosa | B430 Education | $51.7M | 5 |
| Community Health Care Inc | Davenport | — | $51.2M | 9 |
| Clarke University of Dubuque Iowa | Dubuque | B420 Education | $50.4M | 7 |
| PALMER LUTHERAN HEALTH CENTER INC | West Union | E220 Health Care | $50.3M | 13 |
| COMMUNITY CHOICE CREDIT UNION | Johnston | — | $50.3M | 16 |
| IOWA FARM BUREAU FEDERATION | West Des Moines | — | $49.5M | 25 |
| KNOXVILLE COMMUNITY HOSPITAL INC | Knoxville | E22Z Health Care | $48.8M | 6 |
| HOPE HAVEN INC | Rock Valley | — | $48.7M | 8 |
| WHEATON FRANCISCAN HEALTHCARE-IOWA INC | Waterloo | E210 Health Care | $48.5M | 8 |
