| ASCENSION BORGESS HOSPITAL | St Louis | E20 Health Care | $402.3M | 11 |
| ASCENSION SE WISCONSIN HOSPITAL INC | St Louis | E220 Health Care | $388.4M | 22 |
| ASCENSION GENESYS HOSPITAL | St Louis | E22 Health Care | $377.9M | 13 |
| ST ALEXIUS MEDICAL CENTER | St Louis | E220 Health Care | $377.4M | 9 |
| Saint Lukes East Hospital | Lees Summit | E21 Health Care | $376.2M | 13 |
| ASCENSION NE WISCONSIN INC | St Louis | E220 Health Care | $363.3M | 13 |
| MISSOURI VALLEY LINE CONSTRUCTORS NATIONAL ELECTRICAL CONTRACTORS ASSN IN | Kansas City | — | $350.7M | 1 |
| Stifel Charitable Inc | St Louis | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $340.5M | 1 |
| Ascension Health Alliance Professional & General Liability Self-Insurance T rust | St Louis | Y200 Mutual & Membership Benefit | $335.9M | 0 |
| SSM HEALTH CARE GROUP | St Louis | E19 Health Care | $334.9M | 13 |
| COXHEALTH | Springfield | E20 Health Care | $323.1M | 26 |
| St Vincent Medical Group Inc | St Louis | — | $315M | 19 |
| Saint Thomas Medical Partners | St Louis | E32Z Health Care | $310M | 18 |
| MERCY HOSPITAL JOPLIN | Joplin | E20 Health Care | $308.7M | 13 |
| ASCENSION ALL SAINTS HOSPITAL INC | St Louis | E220 Health Care | $308.2M | 19 |
| SOUTHWESTERN LINE CONSTRUCTORS CHAPTER NATIONAL ELECTRICAL CONTRACTORS ASSN IN | Kansas City | — | $306.7M | 1 |
| CARPENTERS HEALTH & WELFARE TRUST FUND OF ST LOUIS | St Louis | — | $302.8M | 0 |
| HANNIBAL REGIONAL HEALTHCARE SYSTEM INC | Hannibal | E220 Health Care | $301.9M | 17 |
| ASCENSION PROVIDENCE | St Louis | E220 Health Care | $301.7M | 14 |
| SKAGGS COMMUNITY HOSPITAL ASSOCIATION | Branson | E220 Health Care | $299.9M | 23 |
| CONCORDIA HEALTH PLAN | St Louis | — | $285.8M | 0 |
| Saint Lukes Health System Inc | Kansas City | E21 Health Care | $283.2M | 45 |
| SSM HEALTH BUSINESSES | St Louis | E920 Health Care | $279.1M | 15 |
| St Vincent Carmel Hospital Inc | St Louis | — | $277.3M | 19 |
| Central Electric Power Cooperative | Jefferson City | — | $269.6M | 23 |
| GOOD SAMARITAN REGIONAL HEALTH CENTER | St Louis | E220 Health Care | $264.5M | 24 |
| St Luke's-St Vincent's Healthcare Inc | St Louis | E22 Health Care | $262.1M | 14 |
| OZARKS MEDICAL CENTER | West Plains | E220 Health Care | $261.4M | 15 |
| Seton Family of Doctors | St Louis | E30 Health Care | $260.9M | 10 |
| FELLOWSHIP OF CHRISTIAN ATHLETES | Kansas City | O550 Youth Development | $260M | 10 |
| Lake Regional Health System | Osage Beach | E220 Health Care | $257.5M | 18 |
| ASCENSION MEDICAL GROUP-SOUTHEAST WISCONSIN INC | St Louis | E22Z Health Care | $254.7M | 17 |
| MERCY HOSPITAL JEFFERSON | Crystal City | E220 Health Care | $252.9M | 16 |
| MERSMISSOURI GOODWILL INDUSTRIES | St Louis | P20 Human Services | $251.7M | 8 |
| ASCENSION PROVIDENCE ROCHESTER HOSPITAL | St Louis | E220 Health Care | $250.4M | 13 |
| BURRELL INC | Springfield | — | $242.6M | 13 |
| LUTHERAN SENIOR SERVICES | St Louis | P75 Human Services | $238.8M | 27 |
| Saint Lukes North Hospital | Kansas City | E21 Health Care | $233.4M | 13 |
| Ascension Health | St Louis | E210 Health Care | $233M | 11 |
| MISSOURI CHAMBER FEDERATION BENEFIT PLAN TRUST | Jefferson City | Y43 Mutual & Membership Benefit | $228.5M | 0 |
| BOILERMAKERS NATIONAL HEALTH & WELFARE FUND | Kansas City | — | $224.6M | 7 |
| A T STILL UNIVERSITY OF HEALTH SCIENCES | Kirksville | B500 Education | $220.6M | 17 |
| COX HEALTH SYSTEMS INSURANCE COMPANY | Springfield | E31 Health Care | $211.8M | 7 |
| SOUTHEAST HOSPITAL | Cape Girardeau | E220 Health Care | $206.8M | 22 |
| THE PATTERSON FAMILY FOUNDATION | Kansas City | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $206.3M | 2 |
| ST ANTHONY SHAWNEE HOSPITAL INC | St Louis | E22 Health Care | $205.5M | 14 |
| UNIVERSITY HEALTH PHYSICIANS | Kansas City | E200 Health Care | $202.3M | 17 |
| SAINT FRANCIS HEALTHCARE SYSTEM | Cape Girardeau | E20Z Health Care | $200.8M | 9 |
| First Community Credit Union | Chesterfield | — | $200.6M | 16 |
| EWING MARION KAUFFMAN FOUNDATION | Kansas City | B82 Education | $196.9M | 13 |
