| SOUTHEAST HOSPITAL | Cape Girardeau | E220 Health Care | $387.9M | 22 |
| ASCENSION SE WISCONSIN HOSPITAL INC | St Louis | E220 Health Care | $385.9M | 23 |
| ST ALEXIUS MEDICAL CENTER | St Louis | E220 Health Care | $383.4M | 11 |
| ASCENSION GENESYS HOSPITAL | St Louis | E220 Health Care | $378.8M | 16 |
| ASCENSION NE WISCONSIN INC | St Louis | E220 Health Care | $377.9M | 13 |
| Saint Lukes East Hospital | Lees Summit | E21 Health Care | $376.2M | 13 |
| MISSOURI VALLEY LINE CONSTRUCTORS | Kansas City | — | $350.7M | 1 |
| Stifel Charitable Inc | St Louis | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $340.5M | 1 |
| SSM HEALTH CARE GROUP | St Louis | E19 Health Care | $334.9M | 13 |
| COXHEALTH | Springfield | E20 Health Care | $323.1M | 26 |
| ASCENSION PROVIDENCE | St Louis | E220 Health Care | $320.1M | 14 |
| CARPENTERS HEALTH & WELFARE TRUST FUND | St Louis | — | $315.6M | 0 |
| ASCENSION ALL SAINTS HOSPITAL INC | St Louis | E220 Health Care | $314.5M | 21 |
| SOUTHWESTERN LINE CONSTRUCTORS CHAPTER | Kansas City | — | $306.7M | 1 |
| St Vincent Medical Group Inc | St Louis | — | $303.3M | 19 |
| HANNIBAL REGIONAL HEALTHCARE SYSTEM INC | Hannibal | E220 Health Care | $301.9M | 17 |
| SKAGGS COMMUNITY HOSPITAL ASSOCIATION | Branson | E220 Health Care | $299.9M | 23 |
| Mercy Clinic Springfield Communities | Springfield | E320 Health Care | $292.1M | 32 |
| Mercy Hospital Joplin | Joplin | E20 Health Care | $286.7M | 11 |
| CONCORDIA HEALTH PLAN | St Louis | — | $285.8M | 0 |
| Saint Lukes Health System Inc | Kansas City | E21 Health Care | $283.2M | 45 |
| ASCENSION ST MARY'S HOSPITAL | St Louis | E20 Health Care | $282.9M | 19 |
| SSM HEALTH BUSINESSES | St Louis | E920 Health Care | $279.1M | 15 |
| St Vincent Carmel Hospital Inc | St Louis | — | $274.7M | 24 |
| Central Electric Power Cooperative | Jefferson City | — | $269.6M | 23 |
| Saint Thomas Medical Partners | St Louis | E32Z Health Care | $263.4M | 19 |
| OZARKS MEDICAL CENTER | West Plains | E220 Health Care | $261.4M | 15 |
| FELLOWSHIP OF CHRISTIAN ATHLETES | Kansas City | O550 Youth Development | $260M | 10 |
| Lake Regional Health System | Osage Beach | E220 Health Care | $257.5M | 18 |
| Providence Hospital | St Louis | E220 Health Care | $256.3M | 14 |
| ASCENSION PROVIDENCE ROCHESTER HOSPITAL | St Louis | E220 Health Care | $256.1M | 15 |
| GOOD SAMARITAN REGIONAL HEALTH CENTER | St Louis | E220 Health Care | $253M | 23 |
| MERSMISSOURI GOODWILL INDUSTRIES | St Louis | P20 Human Services | $251.7M | 8 |
| MERCY HEALTH EAST COMMUNITIES | Chesterfield | X00Z Religion-Related | $248.9M | 13 |
| St Luke's-St Vincent's Healthcare Inc | St Louis | E22 Health Care | $244.1M | 20 |
| CAPITAL REGION MEDICAL CENTER | Jefferson City | E220 Health Care | $242.9M | 12 |
| LUTHERAN SENIOR SERVICES | St Louis | P75 Human Services | $238.8M | 27 |
| Saint Lukes North Hospital | Kansas City | E21 Health Care | $233.4M | 13 |
| ASCENSION MEDICAL GROUP-SOUTHEAST WISCONSIN INC | St Louis | E22Z Health Care | $229.4M | 18 |
| Ascension Health | St Louis | E210 Health Care | $228.5M | 12 |
| BOILERMAKERS NATIONAL HEALTH & WELFARE | Kansas City | — | $224.6M | 7 |
| Ascension Health Alliance Professional & General Liability Self-Insurance T | St Louis | Y200 Mutual & Membership Benefit | $223.9M | 0 |
| A T STILL UNIVERSITY OF HEALTH SCIENCES | Kirksville | B500 Education | $220.6M | 17 |
| Mercy Hospital Jefferson | Crystal City | E220 Health Care | $218.8M | 15 |
| Ascension Welfare Benefits Trust | St Louis | Y43 Mutual & Membership Benefit | $215.9M | 1 |
| MISSOURI CHAMBER FEDERATION BENEFIT | Jefferson City | Y43 Mutual & Membership Benefit | $215.3M | 0 |
| THE PATTERSON FAMILY FOUNDATION | Kansas City | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $211.2M | 2 |
| BURRELL INC | Springfield | — | $211.1M | 13 |
| Mercy Health Springfield Communities | Springfield | E210 Health Care | $207.8M | 18 |
| ALEXIAN BROTHERS HEALTH SYSTEM | St Louis | E210 Health Care | $201.2M | 15 |
